Posted by admin on Jan 31, 2019 in |
The several spectrophotometric and HPLC methods have been reported for determination of Nebivolol and Valsartan individually or in combination with other drugs in pharmaceutical dosage forms. Hence, in the present study, a new, sensitive, suitable and cost-effective Reversed-Phase High-Performance Liquid Chromatography method was developed and validated for the determination of Nebivolol and Valsartan in bulk and tablet formulation. In RP-HPLC method, the analyte was resolved by using a gradient system, methanol and water OPA 0.1% (70:30 v/v) pH 3.5 was used as mobile phase, at a flow rate of 0.7 ml/min, on HPLC system containing UV- detector with Workstation Software and Greece C18 column (4.6 × 250 mm; 5μm). The detection was carried out at 274 nm. The retention time was found to be 5.0500 min and 9.7167 min for Nebivolol hydrochloride and Valsartan respectively. The method gave the good resolution and suitable retention time. The results of the analysis in the method were validated regarding accuracy, precision, specificity, linearity, limit of detection, limit of quantification and robustness. A...
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Posted by admin on Jan 31, 2019 in |
Chytranthus macrobotrys (Sapindaceae) is one of the notable African medicinal plants with traditional history but lack scientific data. This report was aimed to evaluate C. macrobotrys seed (CMS) methanol extract for its phytochemical composition, antimicrobial activity, α-glucosidase, and α-amylase inhibitory activities. Methanolic extract of C. macrobotrys seed was investigated for its chemical composition, antimicrobial activity, α-glucosidase, and α-amylase inhibitory activities. The results revealed the presence of flavonoid (18.61 ± 1.88 mg/100g), phenol (28.71 ± 2.95 mg/100g), tannin (17.29 ± 1.84 mg/100g), terpene (587.07 ± 3.63 mg/100g), sterol (111.54 ± 3.85 mg/100g) and saponin (761.78 ± 1.35 mg/100g). The extract using agar diffusion method showed antimicrobial activity against Staphylococcus aureus, Proteus vulgaris, Pseudomonas aureuginosa, Streptococcus pneumonia, Bacillus cereus, and Micrococcus luteus with a diameter of inhibition zones from 10-20 mm. A weak antimicrobial activity was observed against Escherichia coli and Shigella sp. while no activity was observed against Klebsiella pneumonia and Salmonella typhi. The CMS showed α-glucosidase and α-amylase inhibitory activity at the concentration range of 10-100 µg/ml. The highest...
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Posted by admin on Jan 31, 2019 in |
Background: This study was carried out to investigate qualitative and quantitative phytochemical analysis and free radical scavenging activity of Boerhavia diffusa (root) and Crateva nurvala (bark) along with certain computational ADME (Absorption, Distribution, Metabolism, Excretion) parameters of compounds analyzed from Boerhavia diffusa (root) and Crateva nurvala (bark). Methods: The dried powder of plants were extracted with increasing polarity of solvent namely ethyl acetate, acetone, ethanol and distilled water by a three-step sequential extraction procedure. The chemical compositions of extracts were investigated using UV-Vis, FTIR and PerkinElmer Gas Chromatography-Mass Spectrometry. Ethanolic extract of Boerhavia diffusa and Crateva nurvala was evaluated in vitro for its ability to inhibit the major enzyme activities of GOX and LDH through spectrophotometrically and mode of inhibition were evaluated using Lineweaver-Burk plots while ADME parameters were derived from ADMET server admet SAR. Results: Among all extracts, ethanolic extracts showed the highest phenol, flavonoid, proanthocyanidins, and coumarin content. High DPPH free radical quenching activity was observed in ethanolic, ethyl acetate and acetone extracts of the plant which...
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Posted by admin on Jan 31, 2019 in |
The aim of the present study was to prepare solid lipid nanoparticles (SLNs) of azathioprine to increase its permeability and to develop azathioprine loaded solid lipid nanoparticles (AZA SLNs) based transdermal film for topical administration with the aim of reducing systemic and gastro-intestinal side effects of the drug and achieve passive targeting of drug to the joint due to enhanced permeability and retention (EPR) effect. Azathioprine SLNs were prepared by hot homogenization technique and optimized by 32 factorial design to evaluate the impact of the formulation variables on the dependent variables. Optimized formulation was evaluated for various in-vitro attributes and then incorporated into a transdermal film by a solvent casting method, which was evaluated for various technological properties and ex-vivo permeability study. Size of the optimized SLNs was found to be 113.3 ± 5.09 nm with 0.308 ± 0.008, polydispersity index (PDI) and encapsulation efficiency of 80.28 ± 1.29%. The in-vitro release studies of optimized formulation showed sustained release up to 24 h possessing biphasic pattern with a burst...
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Posted by admin on Jan 31, 2019 in |
Lup-20(29)-en-3-ol, known as Lupeol is one of the most extensively studied triterpene compounds for their diverse biological properties. Natural triterpenoids gain pre-eminence in cancer research owing to their effects in regulating transcription factors and pathways associated with cell proliferation. The objective of the present study was to evaluate the anticancer activity of Lupeol on human adenocarcinomic human cell lines A549, and expression of anti-apoptotic genes. A549 cell line used in the present study as an in-vitro model of non-small cell lung cancer provides an insight into the apoptotic and anti-metastatic potential of Lupeol. Antiproliferative effects and apoptotic potential was measured using 3-(4, 5-dimethyl thiazolyl-2)-2, 5-diphenyltetrazolium bromide (MTT) assay and Acridine orange/Ethidium bromide staining method respectively. Invasion assay was used to mimic an experimental system to determine metastatic activity and effect of Lupeol on apoptosis-related genes was validated with RT-PCR. The results revealed that Lupeol decreased the cell viability in a dose-dependent manner with an LD50 value of 62.53 mg.mL-1. Presence of apoptotic bodies with membrane blebbing suggests the apoptotic...
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