Posted by admin on Nov 30, 2018 in |
Ledipasvir is an inhibitor of the HCV NS5A protein, which is required for viral replication used in the treatment of hepatitis C. Ledipasvir is practically insoluble (less than 0.1 mg/mL) across the pH range of 3.0-7.5 Due to low solubility, it results into poor bioavailability after oral administration. Therefore, solid dispersions (SDs) of Ledipasvir were prepared by two methods, i.e. spray drying technique and hot-melt extrusion by using various carriers like polyvinyl caprolactam-polyvinyl acetate-polyethylene glycol graft copolymer (soluplus), hypromellose 5 cPs and copovidone (kollidonVA64) to increase its aqueous solubility. Faster and high drug release was found in the SDs which was prepared by spray drying technique (SDT) with co-povidone in the ratio of 1:2 as compared with hot melt extrusion (HME) using drug substance and soluplus in the ratio of 1:2. There are 5 folds increases in the solubility of Ledipasvir prepared by SDT and HME compared with plain drug substance. FSD3 and FHM9 are finalized as optimized formulations prepared by SDT and HME based on their solubility, drug...
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Posted by admin on Nov 30, 2018 in |
Free radicals are toxic by products of natural cell metabolism and are responsible for causing a wide number of health problems. Okra is a one of the traditional plant scientifically known as Abelmoschus esculentus Linn. belong to the family Mallow, having rich nutritional value and proved to have many therapeutic uses, various parts of this plant is used in different types of treatment, preparation of pharmaceutical products and also used in preparation of fibers. Scientifically leaf extract of Abelmoschus esculentus proved to have antipyretic, antispasmodic, anti-cancer, immuno modulatory activity. In this study petroleum ether extract, chloroform, Ethanolic extracts of Abelmoschus esculentus Linn. leaves were evaluated in-vitro by experimental parameters such as DPPH scavenging activity, Nitric oxide radical, Hydroxyl radical scavenging, superoxide dismutase scavenging capacity. Abelmoschus esculentus Linn. is used as an antioxidant, antidiabetics, hepatoprotective, cytotoxic activity, genetoxicity, antitumour activity, antilice agent. It is related to contain alkaloids, carbohydrates, fixed oils, tannins and...
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Posted by admin on Nov 30, 2018 in |
Potassium bromate (KBrO3–) is a chemical compound commonly used as a oxidizing agent that has been used as a food additive for baking of white bread, bun, pav and pizza bread. Potassium bromate (KBrO3–) is mainly known to generate free radicals, which cause oxidative damage to essential cellular macro molecules, leading to cause nephrotoxicity in experimental animals. In this part of experiment biochemical profile i.e. alkaline phosphatase, acid phosphatase and protein content along with ovarian histopathological changes were observed. The animals were treated with KBrO3–, 0.7 mg/g b.wt. in 0.125 ml distilled water orally through canulla upto 15 and 30 days. It has been observed significantly lowered ALP, protein and ACP level elevated. However ovarian histophathological changes were not observed after 15 days KBrO3– treatment. Along with the ovary treated with potassium bromate upto 30 days show degenerative cells in the thecal layer and less amount of cytoplasmic was noticed. Our result conclude that in a very low amount, potassium bromate if is available in the body may pose...
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Posted by admin on Nov 30, 2018 in |
In the present work, a rapid, specific, accurate and precise Reversed phase high performance liquid chromatographic (RP-HPLC) method has been developed and validated for simultaneous determination of Dapagliflozin and Saxagliptin in bulk and pharmaceutical dosage form. Successful chromatographic separation of Dapagliflozin and Saxagliptin was carried out with Inertsil-ODS, C18 column (250 × 4.6 mm; 5 μm) with mobile phase consisted of a mixture of Methanol and Potassium dihyrogen phosphate buffer in the ratio of 45:55 v/v delivered at a flow rate of 1.0 ml/min. The eluents are monitored by PDA detector and peaks values were measured at 210 nm. The retention times for Dapagliflozin and Saxagliptin were 4.707 min and 6.684 min respectively. The present analytical method was validated according to ICH guidelines (ICH, Q2 R1). The linearity study of Dapagliflozin and Saxagliptin was found in the concentration range of 20-70 μg/ml and 20-70 μg/ml respectively and coefficient of variance was 0.999 for both drugs. % recovery was found to be 100.37% and 100.16% for Dapagliflozin and Saxagliptin respectively....
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Posted by admin on Nov 30, 2018 in |
The whole plant extracts of N. crociformis and N. infundibulifolia was analyzed by priliminary phytochemical, total phenolics, flavonoid, carbohydrate and alkaloid content by standard methods. In-vitro antioxidant DPPH assay and Phosphomolybdenum assay developed by Braca et al., Prieto et al., respectively. In-vitro antibacterial (agar well diffusion) activity by gram negative Pseudomonas aeroginosa (ATCC 27853), gram positive (Streptococcus mutans MTCC 890 & Staphylococcus aureus ATCC 25923, Esteritia coli ATCC 25922) and antifungal activity by Aspergillus niger (ATCC 16404). The study shows that the petroleum ether, chloroform, ethanol and aqueous extract of the plants contains a good number of phytochemicals such as flavonoid, cardiac glycoside, saponin, alkaloid etc. as well as antioxidant with respect to the scavenging activity against free radicals DPPH which were roughly comparable to that of BHT. The plant extracts also exhibited potent antimicrobial activity against S. aureus, P. aeruginosa, S. mutans, E. coli and A. niger indicating its bactericidal and fungicidal properties. It may be concluded that the plant Nervilia crociformis is chemically more active than Nervilia...
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