Posted by admin on Oct 31, 2018 in |
A new reversed phase simple, economic and specific validated high performance liquid chromatography method for the estimation of empagliflozin in human plasma is presented. A chromatographic separation was carried out isocratically by mobile phase comprising of methanol and acetonitrile, 50:50% v/v at a flow rate of 1.0 ml/min on agilent long C18 column (250 mm × 4.6 mm, 5 µm) with photodiode arrays (PDA) detection at 270 nm. The bioanalytical procedure involves deproteination of plasma with 30% ethyl acetate and liquid-liquid extraction process. Chromatogram showed a peak of empagliflozin at retention time of 8.898 min. The correlation coefficient (r2) was found to be 0.999 in the concentration range of 50-150 μg/ml. No interference peak was observed in blank plasma samples at the retention time of empagliflozin. The percentage of relative recovery and coefficient of variation (CV %) values of precision and accuracy were within the acceptable limits. The method proved simple, cost effective and sensitive for estimation of empagliflozin in human plasma....
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Posted by admin on Oct 31, 2018 in |
The ancient medicine acupuncture was used to treat the diabetes and its complications. Our study was aimed to find out the hypoglycemic effect of Acu-TENS electrical stimulation at bilateral Zusanli (ST36) acupoint in streptozotocin (45 mg/kg/body weight/IP) induced diabetic rats. Diabetes was induced in male albino wistar rats with the administration of single dose of streptozotocin(45mg/kg/body weight/IP). The fasting blood glucose level, body weight, food and water intake were assessed periodically in the experimental animals and its effect was compared with conventional anti-diabetic drug (pioglitazone7.5 mg/kg/body weight). Zusanli acupoints was stimulated with 15HZ of 15MA high intensity and 5 sec pulse width for 5 minutes daily without anaesthesia for 60 days. Acupoint ST36 shows significant reduction (p≤0.01) in blood glucose level and also restored the body weight, food and water intake in treated rats and compared with anti-diabetic drug. The hypoglycemic effect of ST36 acupoint may be due stimulation of energy flow Qi to balance Yin and Yang and utilization of energy due to mild muscle twitching during stimulation....
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Posted by admin on Oct 31, 2018 in |
Over the counter (OTC) drugs are drugs easily available without the prescription of a registered medical practitioner (RMP) and self-mediation practices of these drugs are very common in medical students especially in diarrhea which mostly requires dehydration therapy only. But very few reports are available regarding the use of OTC antidiarrhoeal drugs. The aim of the study was designed to assess the practice of self-medication for diarrhea among second year medical students in RIMS, Ranchi, Jharkhand. This was a cross sectional study conducted among medical students in March-April 2017. Data was collected through self-administered questionnaire and result was expressed as percentage frequency. Questionnaires were filled completely by 224 students out of 269. 81.48% males and 85.07% females were practicing self-medication. Books were the major source of information about drugs and mild nature of illness was the main cause of self-medication practice. The most common drug group used for diarrhea was oral rehydration salt (ORS) and about 41.07% have used self-medication once in past 6 months. In 75% cases diarrhea...
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Posted by admin on Oct 31, 2018 in |
The present study is focused on the electrochemical behavior of glycine 4-bromo acetophenonimine (GBAPI) and its Ni(II) complex. In buffer solutions (Britton-Robinson universal buffer and phosphate buffer) of pH 3 – 9 range, using cyclic voltammetric technique. GBAPI is synthesized by the condensation of 4-bromo acetophenone and glycine amino acid (1:1 molar ratio). The complex is generated by the reaction of nickel (II) acetate and GBAPI in 1: 2 molar ratio in reflexing alcohol. The process is irreversible and diffusion controlled for both GBAPI and Ni(II) complex. The effects of change in pH, solvent and sweep rate are evaluated. Kinetic parameters (charge transfer coefficient (αn), diffusion coefficient (D01/2), rate constant (k°f,h) are calculated from cyclic voltammetric measurements. These compounds are screened for their antibacterial and antifungal activities and the results are compared with those of a standard antibacterial and antifungal...
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Posted by admin on Oct 31, 2018 in |
The leaves of crepe ginger (Costus speciosus (J. Koeng.) Sm. are utilized by the Subanen tribe of Lapuyan, Zamboanga del Norte, Philippines to treat diabetes. However, the hypoglycemic potential of this plant is not yet well-established. Thus, the present study investigated the in-vitro alpha-amylase inhibitory and antioxidant activities of the leaves of C. speciosus. The evaluation of the alpha-amylase inhibitory activity was conducted in-vitro using soluble starch. The 2, 2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity was employed in the evaluation of antioxidant activity. Total phenolics content was investigated using Folin-Ciocalteu reagent. The presence of phytoactive compounds that might contribute to its activities was determined through phytochemical screening and Gas chromatography – Mass spectrometric analysis. The results revealed that the leaf extracts exhibited alpha-amylase inhibitory potential and antioxidant activity which might be due to the presence of bioactive compounds such as eicosane, nonadecane, squalene, phenol, 2, 4-bis (1, 1-dimethylethyl), d-limonene, and hexadecanoic acid, ethyl ester. Negative and low correlation between DPPH scavenging activity and total phenolics content were obtained from both...
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