Posted by admin on Feb 28, 2019 in |
The aim of the present research work is to validate the apixaban content in bulk and pharmaceutical dosage formulation and validate it as per ICH guidelines. A simple, rapid, precise and highly selective spectrophotometric method was developed for estimation of apixaban in tablet dosage form by the area under curve method. The area under curve method involves the measurement of absorbances of apixaban at the wavelength of 269 nm-289 nm. Methanol was used as a solvent. Linearity was observed in the concentration range of 5-25 µg/ml for apixaban. The accuracy of the method was confirmed by recovery studies of tablet dosage forms and was found to be 100% for Apixaban. The method showed good reproducibility and recovery with % RSD less than 0.988%. The LOD of apixaban was found to be 0.335 µg/ml, and LOQ of apixaban was found to be 1.015 µg/ml. Thus the proposed method was found to be rapid, specific, precise, accurate and cost-effective quality control tool for the routine analysis of Apixaban in bulk and...
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Posted by admin on Feb 28, 2019 in |
The current study involves evaluation of antioxidant potential of selected nutraceuticals like barley grass juice (Hordeum vulgare L.), ethanolic extracts of cashew apple (Anacardium occidentale L.) and sea lettuce (Ulva reticulata F.) in in-vitro experimental model using goat liver slices at different concentrations (100, 200, 400, 800 and 1600 mg/kg, p.o). The oxidative stress was induced using hydrogen peroxide, and the levels of lipid peroxidation (LPO), superoxide dismutase (SOD), catalase, and reduced glutathione (GSH) were measured. Rutin was used as a standard. Barley grass juice, ethanolic extracts of cashew apple and sea lettuce have dose-dependently ameliorated the LPO, an indicator of membrane damage caused by oxidative stress. The selected nutraceuticals have significantly improved the levels of antioxidant enzymes like SOD, catalase, and GSH. Although the present study reported the antioxidant potential of the selected nutraceuticals, it still needs to be explored in different test systems employing different...
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Posted by admin on Feb 28, 2019 in |
Background: Chronic periodontitis (ChP) is the most common form of periodontal diseases. The progression rate may be influenced by local, systemic conditions and environmental factors that can alter the normal host response to bacterial plaque and affect the susceptibility to disease. Several studies have reported a role for vitamin D receptor (VDR) gene polymorphisms in numerous immunological activities. The present study purpose was to investigate whether FokI polymorphisms in the VDR gene were associated with the incidence of ChP in a Syrian population. Methods: The study sample comprised eighty Syrian subjects who were allocated into two groups. 50 patients were included in the group (ChP), whereas 30 matched individuals with no periodontal disease were included in the group (R). DNA was isolated from peripheral blood cells. Subsequently, genotyping of the (VDR) polymorphism was performed by a polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP) analysis using FokI enzyme. Statistical analysis was carried out to assess the differences between study groups. Results: Alleles T and C were present at...
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Posted by admin on Feb 28, 2019 in |
Diabetes is a group of metabolic disorders characterized by a chronic hyperglycemic condition resulting from defects in insulin secretion, insulin action or both. The chronic hyperglycemia in diabetes is associated with long term damage, dysfunction, failure of kidney, nerves and blood vessel. Nowadays, herbal treatments available for diabetics are eroding the share of allopathic formulations. Currently, the spray drying technique has been widely used for improving processability, biopharmaceutical performance as well as modifying the physical form of various phyto-extracts. Extract of mulberry being sticky may find difficulty in its formulation to tablet dosage form. Across the world, mulberry has been used for its medicinal importance. Looking at its availability and medicinal prospects spray dried mulberry extract was prepared and which is finally assembled into a tablet dosage form. Inert excipients are used to deal with processability, amorphous form and bioavailability issues. Spray drying of the mulberry extract was carried out by using carrier maltodextrin. Results obtained shows excellent/good flowability, compressibility, and compatibility. The dissolution profile of spray dried extract...
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Posted by admin on Feb 28, 2019 in |
This study reports the potential of a marine mollusk, Chicoreus sp., as a producer of peroxisome proliferator activated receptor-gamma (PPAR-γ) ligand that activate the scavenger receptor class B type 1 (SR-B1) for anti-atherosclerotic activity. The objective of this study is to determine the cytotoxicity of the obtained compound C35, with a molecular weight of 270.1 and the level of SR-B1 expression of a hepatocellular carcinoma cell line (HepG2) after treatment with the compound. Various concentrations of compound C35 were treated against HepG2 to determine its cytotoxicity level (MTS assay) and against transfected HepG2 with SR-B1 promoter (luciferase assay). A non-toxic result obtained in MTS assay where the IC50 is exceeding 100 µg/mL. For luciferase activities, the compound increases the transcriptional regulations of SR-B1 promoter at specific effective concentrations of 12.5 µg/ml with 1.8-fold higher as compared to the positive control. The compound increased the transcriptional regulations of SR-B1 promoter activity and subsequently increased the luciferase activity of the assay system which also reflects the ability of it as a...
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