Posted by admin on Nov 30, 2018 in |
The aim of the present study is to find the effectiveness of two series of indenoisoquinolines compounds against cancer. Topoisomerase I (Top 1) an essential enzyme that participates in the processes associated with separation of DNA strands and manages the super helical tensions through the transient breakage of one strand of duplex DNA, monitored by the unwinding of supercoiled DNA. The Top 1-DNA complex an effective target in the treatment of cancer was taken as a receptor for study. The two series of indenoisoquinolines compounds were taken as ligand molecules and the 3D structure of human Top1-DNA was retrieved from PDB database. The structures of ligand were drawn using Chemsketch. Docking studies were performed using GOLD which predicts the binding affinity and hydrogen bond interactions between the ligand and receptor. From the results, it was observed that the selected series of both nitrated indenoisoquinolines and 2, 3 dimethoxy-substituted indenoisoquinolines had better Gold score with the active site residue ARG364. Docking studies conclude both the series may act as potent...
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Posted by admin on Nov 30, 2018 in |
The objective of the study was to investigate the effect of microemulsion on in–vitro skin permeation, percutaneous absorption of Irbesartan and to postulate probable mechanism of skin permeation. Microemulsion was prepared by aqueous phase titration method. Pseudoternary phase diagrams were constructed for the microemulsions. Thermodynamic stability study was carried out on selected microemulsions. Characterization of selected Irbesartan-loaded microemulsions was done by droplet size and polydispersity index determination. Physicochemical properties of Irbesartan-loaded microemulsions were determined using reported procedures. In-vitro skin permeation was determined using Franz cell. Skin permeation mechanism was assessed by Fourier Transform Infra – Red spectra analysis, Differential Scanning Calorimetry and activation energy measurement. A non-compartmental pharmacokinetic study was carried out to estimate the percutaneous absorption through the rat skin. The particle size and polydispersity index of the spherically shaped microemulsions were in the range of 83 – 86 nm and 0.270 – 0.290 respectively. In-vitro skin permeation study gave the permeability coefficient and steady state flux of Irbesartan to be 3.52 (×10-3) cm/h and 17.59 ± 0.38...
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Posted by admin on Nov 30, 2018 in |
A new Polyherbal Mouth Rinse was formulated comprising of polyherbal extracts of grapes peel, neem, clove, fennel and ginger based on their traditional applications. The extracts used in the formulation and polyherbal mouth rinse both were subjected for anti-bacterial activity separately. Phytochemical tests and spectral studies of the extracts have indicated the presence of polyphenolic compounds- mainly trans-resveratrol and the UV-Vis spectrum also shown the presence of trans-resveratrol at 260 nm. This compound is reported to have wide applications. Both the extracts and polyherbal mouth rinse have shown good sensitivity towards microorganisms like S. aureus, S. mutans and E. fecalis. These study results shows that the presence of compounds like glycosides, terpenoids, flavanoids and saponins which may be responsible for the antibacterial efficacy of the extracts and polyherbal mouth rinse against the these microorganisms which are mostly found responsible for bacterial infections in oral cavity. However further study is needed to confirm...
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Posted by admin on Nov 30, 2018 in |
The present study was aimed to evaluate the effects of repeated administration of methanolic leaf extract of Pterospermum acerifolium Linn. Willd. (MEPA) on the reproductive system of male and female Wistar albino rats and its developmental effects on subsequent generation. MEPA was administered to Wistar albino rats by gavage for 28 days repeatedly at doses of 250, 500 and 1000 mg/kg body weight, in accordance to OECD guidelines 414 (developmental study) and 416 (reproductive study). Male fertility was estimated by sperm count. Biochemical evaluations included estimation of glycogen content, superoxide dismutase activity and ascorbic acid. Body weight and food intake behavior was also monitored regularly. MEPA treated male and female rats were allowed to mate. After mating females were observed for signs of parturition and allowed to give birth to the F1 generation. The pups were clinically observed. At the dose levels tested MEPA does not significantly (p<0.01) alter sperm count of male rats. Female reproductive system tolerates MEPA well. Normal gestational period was observed. Normal birth rates of...
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Posted by admin on Nov 30, 2018 in |
Introduction: Oral submucous fibrosis (OSMF) is a potentially malignant disorder carrying a high risk of malignant transformation. A wide range of treatment modalities have been proposed for OSMF but none have proved curative or reduced the morbidity significantly. In-vitro and in-vivo studies suggested curcumin as an anticancer, antioxidant and anti-inflammatory agent. Thus based on this literature survey the study was undertaken. Aim: To evaluate the efficacy of curcumin for the treatment of clinical stage 2 OSMF patients and to compare it with patients receiving placebo drugs. Study Design and Sample Size: A randomized single blinded placebo controlled clinical trial was conducted in 100 clinical stage 2 OSMF patients with clinically and histopathologically confirmed diagnosis. Materials and Methods: 100 clinical stage 2 patients selected for the study were divided into 2 groups with 50 patients each. Group 1 patients were given placebo capsules. Group 2 patients were given curcumin capsules of 500 mg. The primary outcome measures were to note the subjective symptoms and objective parameters. These parameters were analyzed...
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