Posted by admin on Feb 28, 2019 in |
Piperine has been widely used as a bioenhancer. Secnidazole is a long-acting 5-nitroimidazole analog. It is used in the treatment of amoebiasis, giardiasis, trichomoniasis and anaerobic bacterial infection. In this study derivative of piperine was synthesized and studied for its bio enhancing the effect and this effect was compared with the bioenhancing effect of piperine. The pharmacokinetic profile of Secnidazole alone and in combination with piperine and piperine derivative was investigated by validated bioanalytical HPLC method as per USFDA guidelines. It was observed that the synthesized derivative of piperine significantly improved the bioavailability of Secnidazole compared to piperine alone in Wistar rats. The N,N-diallyl-5-(benz)(1,3)dioxol-5-yl)penta-2,4-dienamide (DA) derivative showed 1.49 fold increase in the bioavailability of Secnidazole. After oral administration of Secnidazole alone and in combination with piperine and piperine derivative (0.035 mmol, 10 mg/kg) there was a significant increase in AUC and Cmax of...
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Posted by admin on Feb 28, 2019 in |
Objectives: Sickle cell disease (SCD) is a common hereditary blood disease with a progressive clinical course and multiple end organs damage. Due to its intercalated pathophysiology, several trials have been conducted targeting its pathophysiology in an attempt to modify the clinical course and minimize complications. Metformin and rosuvastatin are known to have pleiotropic effects such as antioxidant, anti-inflammatory and vasculoprotective properties making them a candidate for testing in SCD. Aim: To test possible effects of metformin and rosuvastatin on oxidative stress, erythrocytes deformability, %hemolysis in blood samples of patients with SCD. Materials and Methods: Blood samples were obtained from 36 SCD patients at stable conditions. The samples were incubated with metformin (1 mg/ml) or rosuvastatin (0.25 mg/ml). Oxidative stress was induced by incubating the samples with H2O2 for 30 min. Reduced glutathione (GSH), lipid peroxidation products (thiobarbituric acid reactive species TBARS), % hemolysis was determined by spectrophotometer and erythrocytes deformability by filtration method. Result: Treatment of blood with metformin or rosuvastatin resulted in a significant reduction in TBARS level...
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Posted by admin on Feb 28, 2019 in |
Present work has been conducted to evaluate antibacterial and antiviral activity of silver nanoparticles using marine Actinomycete, Nocardiopsis alba, isolated from mangrove soil. Formation, size, and shapes of silver nanoparticles (AgNPs) were characterized by UV-visible spectroscopy, X-Ray Diffraction (XRD), FTIR and Transmission electron microscope (TEM). From the UV-visible spectroscopy, the absorption peak was found at 420 nm. The SEM images confirmed that the sample contains spherical silver nanoparticles. The XRD analysis confirmed that the silver nanoparticles are crystalline, which was confirmed by the FT-IR peak at 564 cm-1 corresponding to the silver nanoparticles vibration present in the crystalline structure. The silver nanoparticles have significant antibacterial activity against Pseudomonas aeruginosa, Klebsiella pneumonia, Streptococcus aureus, and E. coli. and antiviral activity against new castle viral disease (NDV) in cattle The findings of the present study suggest that the silver nanoparticles possess a good antibacterial a d antiviral activity and could have great importance as a therapeutic agent in current...
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Posted by admin on Feb 28, 2019 in |
Proteins play a significant role in living beings. In animals, where proteins are involved in the body built, the formation of tissues, organs, etc., in plants, proteins regulate the respiration, photosynthesis, growth, and reproduction. There are some proteins which are specific to the plant or a particular species of plant. Proteomics is a multidisciplinary branch of science that deals with the study of proteins. The paper discusses some important aspect related to estimation and identification of proteins. Protein was estimated in two plants of forensic importance, i.e., Ricinus communis and Cannabis sativa seeds by Ultra Violet (UV) spectrophotometry followed by isolation and characterization of proteins by Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS PAGE) method. A UV spectrum was taken at 520 nanometers (nm), and the protein was quantified by the Folin-Ciocalteu method. SDS-PAGE was performed according to Laemmli et al. High concentration of protein was found by UV spectrophotometry at 520 nm. SDS-PAGE separated the proteins according to their molecular weight ranging in size from 45 kiloDalton (kDa) to...
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Posted by admin on Feb 28, 2019 in |
Objective: This study aimed at ensuring the different compositions of Valli panchmoola using authentic herbs and establishing pharmacognostical, physicochemical, and phytochemical standards for the compositions. Methods: Compositions of Vallli panchmoola were prepared as per the formula and procedure mentioned in traditional texts and Ayurvedic Formulary of India. The prepared compositions were evaluated for pharmacognostical, physicochemical, and phytochemical parameters using guidelines of the World Health Organization and Pharmacopoeial Laboratory for Indian Medicines for quality control of herbal drugs. Results: Microscopic studies revealed the presence of collenchyma cells, rosette crystals, compound starch grains, parenchyma cells, cluster crystal, etc. Loss on drying 0.17 ± 0.01%, total ash 1.87 ± 0.02%, etc. were observed in the classical group. Whereas loss on drying 0.10 ± 0.04%, total ash 1.77 ± 0.02%, etc. were observed in group-1. Preliminary phytochemical screening confirms the presence of alkaloids, flavonoids, glycosides, fatty acids, terpenoids, sterols, tannins, proteins, and phenolic compounds. Conclusion: The various pharmacognostical, physicochemical, and phytochemical standards will help in quality control/quality assurance and maintaining batch to batch...
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