Posted by admin on Oct 31, 2018 in |
Like most other plants Citrus paradisi contain various secondary metabolites with great potentials. The aim of this paper is to evaluate the phytochemicals by using quantitative and qualitative analysis of ethyl acetate, ethanol, n-hexane and aqueous extracts with the help of standard techniques. The findings from quantification and phytochemical screening showed the presence of alkaloids, flavonoids, reducing sugars, Phenols, proteins, amino acids, saponins, tannins, terpenoids, and glycosides. Further, the study findings revealed that ethanolic extract of fruit extract was found to have more constituents when compared with other extracts by quantitative method. Elemental analysis showed the presence of selenium (1.56 mg); manganese (4.95 mg); iron (5.12 mg); magnesium (34.5 mg) and zinc (9.85 mg). Chromatogram of flavonoid standards such as rutin, quercetin, gallic acid, hesperidin and ethanolic extract of Citrus paradisi showed the high amount of naringin. Hence the ethanolic extract of Citrus paradisi shows many compounds and may have been used in traditional medicine for prevention of several...
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Posted by admin on Oct 31, 2018 in |
Berberine hydrochloride is an isoquinoline alkaloid isolated in the form of Berberine hydrochloride from the roots and stems of Berberis vulgaris, B. aristata, Coptidis rhizoma and other plant species. Berberine is well known for its antiproliferative and anti inflammatory activity since long time. The present study is carried out to study mechanism of inducing apoptosis by berberine hydrochloride on HEPG2 liver cancer cell line. MTT assay used to study cytotoxicity against HEPG2 cells and COX2, PGE2 and western blot were used to study its apoptotic pathway. PGE2 has been implicated in COX-2-mediated effects; we determined the levels of PGE2 in the berberine-treated cells. Our results revealed that treatment with berberine hydrochloride for 24 h resulted in significant inhibition of PGE2 production in a dose-dependent manner 21-75%, P, 0.01-0.001, suggesting that berberine induced reduction in PGE2 production is associated with an inhibitory effect of the berberine on COX-2 in these cells. Berberine has shown PPAR Gamma activation and COX2 inhibition via PGE2 regulation and caused apoptosis by down regulating anti-apoptotic...
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Posted by admin on Oct 31, 2018 in |
Aim of the Study: The aim of this study was to investigate the antilithiatic activity of ethanolic leaves extract of Dalbergia sissoo Linn. ethylene glycol induced in rats. Materials and Methods: All the chemicals were analytical grade. Cystone was obtained from from Himalaya Health Care Ltd. The ethanolic extract of plant Dalbergia sissoo Linn. leaves were given with tween 80 as vehicle administered orally. All chemicals were used of analytical grade available; ethylene glycol was obtained from Merck Ltd., Mumbai, India. Male Albino rats of wistar strain weighing between 150-200 g were selected for the antiurolithiatic activity. Animals were housed in polypropylene cages with the filter tops and maintained at 25 ± 2 ºC, relative humidity 55 ± 10% under controlled conditions of 12-h light: 12-h dark cycle. Results: In the present in-vivo study DSEE (at 100 and 200 mg/kg) exhibited a dose dependent significant anti-lithiatic activity on treatment. The extract dose of 100 mg/kg also caused reduction of calcium, oxalates, phosphorus and creatinine in blood serum level the...
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Posted by admin on Oct 31, 2018 in |
The approach of site specific topical delivery of Rizatriptan Benzoate (RBZ), used for migraine using the elastic carrier at posterior cervical region for sustained action was attempted in the present study. RBZ-entrapped elastic niosomes were prepared using the modified mechanical stirring method. The formulation was optimized using 23 full factorial designs. The type of edge activator (EA), Tween: cholesterol: edge activator molar ratio (T:C:EA) and Total surfactant concentration (S) as independent and the percent entrapment efficiency (%EE) as dependent variables with visual stability of dispersion for two week as constrain factor for selection. The study revealed that interaction between EA and S was influencing factor for %EE. The optimized formulation EN3 having edge activator type, total surfactant concentration and molar ratio Tween 20: cholesterol: EA as Sodium cholate, 2% and 2:1:2 respectively, having high percent entrapment efficiency (92.22%), showed unilamellar smooth surface in SEM study. Zeta potential was found to be – 17.2 mV. In-vitro permeation from the elastic niosomal gel followed zero order model over a period of...
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Posted by admin on Oct 31, 2018 in |
In the current study, synthesis of silver nanoparticles (AgNPs) using swertipunicoside was tested against human breast cancer cell line (MCF-7). The swertipunicoside was tested against the normal cell line such as vero cells to find out the toxicity of the compound. The synthesized AgNPs were characterized using UV-visible spectroscopy, X-ray powder diffraction (XRD), fourier-transform infrared spectroscopy (FTIR) and field-emission Scanning Electron Microscope (FESESM) with energy dispersive spectroscopy (EDAX). The synthesized silver nanoparticles were checked with the colour variation and it was confirmed by UV-vis spectral analysis. The morphology of the synthesized nanoparticles were analysed using FESEM along with EDAX. The XRD was done to find out the crystalline structure of the compound. FTIR measurements are carried out to identify the possible biomolecules responsible for capping and efficient stabilization of the silver NPs synthesized using swertipunicoside. The synthesized AgNPs and swertipunicoside was tested against the MCF-7 cell line to find out the cell viability. Our results showed that the compound and synthesized silver nanoparticles inhibited the proliferation of human breast...
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