Posted by admin on Dec 31, 2018 in |
This study developed a stability-indicating reversed-phase high-performance liquid chromatography method for simultaneous estimation of sofosbuvir and ledipasvir of bulk drug and formulation by using a QbD approach. Literature survey reveals that there were very few analytical methods available for the simultaneous estimation of sofosbuvir and ledipasvir. An attempt was made to develop and validate stability indicating assay method using RP-HPLC through QbD approach. In a QbD approach, Box-Behnken screening based on critical method parameters, i.e. (Buffer pH, organic phase-% methanol and flow rate). The interaction effect of these parameters on the response variables (Retention Time, NTP and tailing factor) were evaluated through 3D response graphs. The plots revealed the final chromatographic conditions of the method. The chromatographic separation was achieved using C18 inertsil ODS-2, 250 mm × 4.6 mm × 5µ column, buffer ammonium acetate and PDA detector at 254 nm. The developed method was successfully applied for the determination of the two drugs from its pharmaceutical formulation as well as successfully applied for forced degradation studies. Force degradation...
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Posted by admin on Dec 31, 2018 in |
Osbeckia L. of Melastomataceae are unique flowering plants showing diversity in their habit and morphological features adapted to their habitats. Limited studies were recorded with the anatomy and morphology of high elevation species. The micromorphology and anatomy of vegetative and reproductive structures of seven species and three varieties of O. aspera, representing diverse habitats such as altitude, availability of water and intensity of sunlight out of the twenty species of Osbeckia reported from India were narrated in the present study. An apparent type of herkogamy noticed in Osbeckia species was also recorded. Herkogamy in this family is an important feature of phylogenetic importance. This aspect of the flowers of Osbeckia appears to represent a special type of herkogamy. The most evident type of herkogamy present in Melastomataceae was represented by flowers that have styles exerted beyond the stamens. Styles and stamens that were about the same length but bent opposite from each other were noticed in some species. Based on the comparison of herkogamy in flowers of many species,...
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Posted by admin on Dec 31, 2018 in |
The objective of the study was to evaluate the antidiabetic activity of methanolic extract of Simarouba glauca in STZ induced diabetes in rats for type I diabetic mellitus. The leaves of Simarouba glauca were dried under shade and then powdered, and extracted with 90% methanol by reflux. Preliminary phytochemical studies and acute toxicity studies were also carried out on a methanolic extract of Simarouba glauca. After overnight fasting, for type I diabetes was induced in rats by i.p. Injection of STZ, at a dose of 65 mg/kg/b.w. Group A served as normal control while group B was considered as diabetic control. Group C was standard receiving insulin 4 U/kg, and Group D diabetic animals were treated with Simarouba glauca (250 mg/kg and 500 mg/kg). During the study, body weight and fasting blood glucose level were taken at 0 and 30th day. At the end of the study, animals in all groups were sacrificed; blood sample, pancreas, and liver were collected – biochemical parameters such as lipid profiles, SGOT, SGPT,...
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Posted by admin on Dec 31, 2018 in |
The purpose of the present study describes the preparation of pH based in-situ gel formulations were applied for the harmful bacterial infections by using carbopol 934P and HPMC derivatives. The simple procedure was adopted for preparing these formulations. To eliminate or reduced adverse systemic effects produce from bacterial conjunctivitis, topical route of administration, such as eye drops was preferred for this type of infections and it should be installed into the eye’s cornea. These eye drops are more effective to provide therapeutic index and also to achieve good bioavailability in the eye. Forthcoming, the developed in-situ gel formulations were characterized by gelling capacity, pH, clarity, appearance, viscosity determination, surface tension, drug content, in-vitro drug release, HET-CAM study, permeation study, and in-vivo ocular irritation study. The optimized formulation (F2) was set up to be non-irritant and well tolerable from the results both in-vitro HET-CAM study and ocular irritation study. The developments in-situ gel formulation forms good gelling capacity, clear/translucent in appearance, after administration in the eye. The developed in-situ gel...
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Posted by admin on Dec 31, 2018 in |
Neuroprotection is utmost necessary due to the devastating neuronal injuries, including neurodegenerative disorders. N. sativa L. is called the Miracle herb due to its wide usage in traditional systems of medicine, especially Arabic. This plant has been studied for its therapeutic potential and found to possess a wide spectrum of activities, including anticancer and antioxidant properties. The present work is designed to investigate the acetylcholinesterase inhibitory and cytotoxic potential of various grades of black cumin oil for its neuroprotective activity. Thymoquinone is the most pharmacologically active ingredient found in black cumin oil. The various grades of black cumin containing 5%, 2% and 0.6% thymoquinone at different dose levels is used throughout this work. Acetylcholinesterase inhibitory is performed by using Ellman’s method, and the cytotoxic potential is evaluated by using Sulforhodamine B assay employing IMR-32, U373-MG, and SK-N-SH cell lines. Black cumin oil containing 5% thymoquinone showed a dose-dependent increase in the acetylcholinesterase inhibition (64.47% inhibition at the dose of 20 µg/ml) which is similar to that of the standard...
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