Posted by admin on Oct 31, 2018 in |
To avoid problems of conventional therapy of drug delivery and reduced dose, sustained release matrix tablet of Bosentan was prepared using lipid base material as matrices. Primary screening of polymer was done by selecting different lipid base materials like Compritol 888 ATO, Precirol ATO 5, Eudragit RSPO, Glycerly monostearate (GMS) and cetosteryl alcohol. All the batches were prepared by direct compression method. Theoretical drug release profile was carried out for dose calculation up to 24 h. All the batches were evaluated for hardness, weight variation, thickness and friability (physicochemical parameters). In-vitro drug release and FTIR study was carried out along with experimental design. From the drug release profile it was observed that Compritol 888 ATO (F1) shows batter retardant effect and Precirol ATO 5 (F2) shows effective burst release. But remaining formulations (F3-F5) were not able to release the drug as per theoretical drug release profile. After selecting lipid matrices it was optimized by 32 full factorial design by applying analysis of variance (ANOVA). Concentration of Compritol 888 ATO...
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Posted by admin on Oct 31, 2018 in |
Biopolymers are gaining more importance in our day-to-day life, due to the hazardous effect of synthetic plastics in our environment. Curdlan is one of the biopolymers, which has a broad field of applications in the food, pharmaceutical and medical industries. It is a polymer of glucose, produced by the family members of Rhizobiaceae. Curdlan [(1→3)-β-D-glucan] production takes place under the nitrogen starvation period during the cell growth. Fifty Agrobacterium sp. were isolated from root nodules collected from different localities of Coimbatore and Palakkad and screened by Aniline Blue method for the production of curdlan. The species was confirmed as Agro-bacterium fabrum by 16S rRNA sequencing. The accession number given by GenBank is MF521602. Curdlan was produced using different carbon and nitrogen sources. The extraction was done by Sodium hydroxide: Acetic acid precipitation. 0.12 gm of crude curdlan was obtained from 1.2 gm/100 ml of biomass. The product was stored for further...
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Posted by admin on Oct 31, 2018 in |
Mosquitoes are becoming a serious threat to the humans causing infectious diseases like dengue, malaria, chikungunya, yellow fever etc. They multiply in large numbers in a polluted environment and stagnant water bodies. The control measures include the use of pesticides and chemical compounds which further causes harmful effects. Thus, an alternative solution is to use a biologically active compound which has larvicidal property and is eco-friendly. Biosurfactants are such compounds. In this present study, 75 isolates obtained from petroleum contaminated soil samples were screened and one potent isolate was selected and identified. The biosurfactant was produced from Bacillus subtilis B50. The crude compound was characterized as lipopeptide. Its larvicidal activity was tested using the similar stage of mosquito larvae at different concentration of crude biosurfactant (1 – 10 mg %). The LC50 and LC100 were calculated after observing the larvae for 72 h. The obtained results showed that as the time and concentration increases the mortality also increases. The maximum number of larvae was killed at a concentration of...
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Posted by admin on Oct 31, 2018 in |
A simple, selective, rapid, precise and economical reverse phase high performance liquid chromatographic method has been developed for the simultaneous estimation of hydrocortisone and ketoconazole from pharmaceutical formulation. The method was carried out on a C18 (25 cm × 4.6 mm i.d., 5 μ) column with a mobile phase consisting of methanol: water (adjusted to pH 3.0 using triethylamine) in the ratio of 70:30 v/v. The retention time of hydricortusone and ketoconazole was 3.50 min and 6.00 min respectively with the flow rate of 1mL/ min. Eluents were detected at 221 nm. The linear regression analysis data for the linearity plot showed good linear relationship with correlation coefficient value for hydrocortisone and ketoconazole were R2 = 0.9995 and R2 = 0.9996 in the concentration range of 10 – 40µg. mL-1, 20 – 80 µg. mL-1 respectively. The relative standard deviation for intra-day precision was lower than 2.0 %. The method was validated according to the ICH guidelines. The method was also found to be robust. The developed method was...
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Posted by admin on Oct 31, 2018 in |
Condom is usually made up of thin rubber (latex) designed or is made of polyurethane to cover the penis sheath at closed one end that is inserted intravaginally during sexual intercourse for contraceptive purpose or as a means of preventive sexually transmitted diseases. Condoms should be safe, effective, user-controlled, non-prescription, easy to use and relatively cheap. The quality of the four different brand of condoms available in the local market was evaluated using standards given by international standards organization (ISO), such as; Bursting volume and pressure (inflation), width, length, thickness, electrical hole test, package integrity, lubricant quantity were done for each brand of condoms. The result showed that all sample of condoms have fulfilled the standards requirement set by ISO 407 and that all four brands of condom which is marketed in Addis Ababa Ethiopia are of safe, effective for the...
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