Posted by admin on Nov 30, 2018 in |
Polycystic ovary syndrome (PCOS) is one of the most common disorders that occur in women at any age due to the endocrine hormone imbalance. The cause for this disorder is still not identified, but on recent research provided that disorder may be caused by some genetic variation. Predicting and understanding the effects of genetic variation that occurred in the gene are becoming more important for single nucleotide polymorphism to understand the molecular basis of genetic disease. From the literature survey, the candidate gene which is responsible for causing genetic PCOS was identified. In this work using computational methods, this candidate gene was analyzed completely to find out the genetic variation which in charge for altering the expression and the functional of the gene. On analyzing the gene, it was predicted that the protein which was translated from the gene played a key role for causing the major alteration in the gene. Using SNP analysis tool, further investigation were carried out to the disease causing protein and were predicted that...
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Posted by admin on Oct 31, 2018 in |
Involvements of oxidative damage, deficiency of acetylcholine and cognitive impairment have been reported in the pathophysiology of epilepsy. It has been demonstrated that cholinesterase is degranulated at the site of a lesion it cause brain disorder leading to epilepsy. Cholinesterase inhibitor (Galantamine) inhibits the release of cholinesterase thus it benefits in epilepsy. So the aim of the study was to evaluate both effect cholinesterase inhibitor (Galantamine) and cognitive behaviour in epilepsy induced by increasing current electroshock seizures test of Wistar rats. Epilepsy was evaluated by behavioural tests such as spontaneous alternation behaviour and Rota rod test. Measurement of oxidative stress was done by various biochemical estimations namely lipid peroxides (in brain), protein estimation using Folin’s reagent and brain reduced glutathione estimation. All the results of galantamine (0.5 mg/kg & 1 mg/kg) were compared to the standard drug sodium valporate (100 mg/kg & 200...
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Posted by admin on Oct 31, 2018 in |
Wounds may harbour diverse microorganisms, especially bacteria that are resistant to many conventional antibiotics. The aim of this study is therefore to evaluate the antibiotic activities of Aloe vera gel and honey against bacteria isolates from wounds. A cross sectional study of wound aspirates from a health care center was carried out to evaluate the antibacterial activities of Aloe vera and honey against bacteria isolates from wounds following standard microbiological procedures. The antimicrobial susceptibility of the isolates to standard antibiotic discs, Aloe vera and honey were done using the agar-diffusion method. The minimum inhibitory concentration of the Aloe vera gel and honey (alone and in combination) were also evaluated using the agar-diffusion method. The bacterial isolated were: Escherichia coli, Staphylococcus aureus, Proteus vulgaris, Staphylococcus epidermidis, and Streptococcus pyogenes. The study showed that honey had a higher antibacterial activity than Aloe vera gel with an inhibition zone diameter (IZD) measuring mm. It also showed that Staphylococcus aureus was the most predominant pathogen found in wounds while Proteus vulgaris and Staphylococcus...
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Posted by admin on Oct 31, 2018 in |
This study planned to screen preliminary phytoconstituents, total phenolic- flavonoids content and in-vitro, in-vivo antioxidant activities of aqueous crude extract of Camellia sinensis leaves. We assessed phytochemical constituents in extract using previously reported methods for tannins, phenols, flavonoids, glycosides, alkaloids and saponins. Quantitative estimation of flavonoids and phenolic compounds was carried out by aluminium chloride and Folin Ciocalteu method. ABTS, DPPH and FRAP methods are used for in-vitro assessment of antioxidant potential; meanwhile extract was tested for antioxidant capacity in Guinea pig plasma using ORAC, MDA and GSH assay in dose dependent manner. Total phenolic and flavonoid content of the extract was found to be 251.77 ± 6.59 µg/mg and 246.56 ± 2.58 µg/mg respectively. Extract shown 93.76 ± 12% antioxidant activity with ABTS and 95.13 ± 17.89% when tested with DPPH hydrogen reacting radicals. While, it was calculated 92.82 ± 8.72% for FRAP assay. The extract shows dose dependent decrease in MDA level (P<0.05, ANOVA) in plasma. Although, the extract produced dose depended increase in GSH value 20.97...
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Posted by admin on Oct 31, 2018 in |
A new reversed phase simple, economic and specific validated high performance liquid chromatography method for the estimation of empagliflozin in human plasma is presented. A chromatographic separation was carried out isocratically by mobile phase comprising of methanol and acetonitrile, 50:50% v/v at a flow rate of 1.0 ml/min on agilent long C18 column (250 mm × 4.6 mm, 5 µm) with photodiode arrays (PDA) detection at 270 nm. The bioanalytical procedure involves deproteination of plasma with 30% ethyl acetate and liquid-liquid extraction process. Chromatogram showed a peak of empagliflozin at retention time of 8.898 min. The correlation coefficient (r2) was found to be 0.999 in the concentration range of 50-150 μg/ml. No interference peak was observed in blank plasma samples at the retention time of empagliflozin. The percentage of relative recovery and coefficient of variation (CV %) values of precision and accuracy were within the acceptable limits. The method proved simple, cost effective and sensitive for estimation of empagliflozin in human plasma....
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