Posted by admin on Oct 31, 2018 in |
Aim: To standardize Amritaprasha ghrita. Materials and methods: Physico‐chemical studies like refractive index, specific gravity, acid value, saponification value, iodine value, determination of unsaponifiable matter, peroxide, viscosity, rancidity test and HPTLC were carried out as per the WHO guidelines, Indian Pharmacopoeia and Ayurvedic Pharmacopoeia. Conclusion: Standardization tests done on Amritaprasha ghrita helped in authenticating and ensuring the quality of the...
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Posted by admin on Oct 31, 2018 in |
Proteases are one of the most important enzymes in many industrial applications, particularly as additives in laundry detergent industry. Hence, the present study investigates the isolation and purification of an extracellular protease from the Bacillus subtilis ASASBT isolated from termite soil sample. Among the five protease producers, one of the isolates was selected for further studies. It was related to Bacillus subtilis on the basis of 16S rRNA gene sequencing and biochemical properties. The maximum protease activity recorded at 48 h of incubation at 37°C was 154.13 U/ml. Based on the maximum activity of the enzyme, further optimization studies were done with Bacillus subtilis ASASBT. Its optima pH and temperature of enzyme production were pH 7.0 and 40 °C, respectively. Starch and gelatin were the best carbon and nitrogen sources for the production of protease. The isolated protease was purified in four steps involving ammonium sulphate precipitation, dialysis, DEAE-Cellulose and sephadex G-100 chromatography had a 4.01 fold increase in specific activity (86.51 U/mg) and 23.73 % increase in recovery...
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Posted by admin on Oct 31, 2018 in |
Background: The incidence of Diabetes Mellitus has increased dramatically in recent decades. Di Peptidyl Peptidase 4 inhibitors (DPP-4) have their role in glycemic control. An impaired ‘incretin effect’, occurs in patients with type 2 Diabetes Mellitus in response to glucose intake. Objectives: The main objective of the study is to compare therapeutic outcomes and adverse drug reactions among commonly prescribed anti diabetic drug combinations in adults with type 2 diabetes mellitus. Methodology: A retrospective and prospective study experimental study was carried out for a period of one year at care diabetes centre. Results: FBS and PLBS were found to be significantly lower in DPP group when compared with SU and TZ. Adverse events such as itching, abdominal pain, constipation and weight loss are more in DPP when compared with other groups. Data from this study indicated that DPP-4 inhibitors are superior to sulfonylureas (SU) and thiazolidinediones (TZ) when used in combination with metformin (B) in glycemic control. Conclusion: DPP inhibitors provide an effective therapeutic option for individuals with Type...
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Posted by admin on Oct 31, 2018 in |
Background: A Schiff base, a pharmacologically privileged scaffold and a nitrogen analog of an aldehyde or ketone in which the C=O group is replaced by C=N-R group. Mutual prodrug is a Carrier linked prodrug consist of two pharmacologically active substances together in a single moiety. Objective: The goal of the study is to synthesize combined form of the various Schiff’s bases with Naproxen for enhanced biological and pharmacological activity. Methods: Various conventional as well as green methods are incorporated for synthesis of Schiff’s bases whereas; simple shaking method is adapted for mutual prodrug synthesis. Biological evaluation was done according to literature. Results and Conclusion: Besides Naproxen’s important pharmacological activities, it possesses severe side effects due to certain functional groups present in it. Thus, derivatization method was incorporated to shun the side effects of the drug by synthesizing its Mutual prodrugs with Schiff bases having additional antibacterial...
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Posted by admin on Oct 31, 2018 in |
To investigate the effect of ethanolic extracts of leaf and fruit of Trichosanthe dioica (T. dioica) and leaf of Clitoria ternatea (C. ternatea) on the biochemical parameters in streptozotocin (STZ) induced diabetic Wistar albino rats. Healthy male adult Albino rats of Wistar strain were randomly divided into eleven groups of six rats each were assigned into normal and diabetic groups (Group I to XI). Diabetes was induced in albino rats by administration of STZ (60 mg/kg i.p). Group I and II were kept as normal control and diabetic control. The other diabetic groups from group III to group X were treated with either individual and combined ethanolic extracts of T. dioica and C. ternatea at a dose of 200 and 400 mg/kg of body weight (bw) were administrated at a single dose per day and group XI was treated with glibenclamide at a dose of 600 μg/kg body weight for 28 consecutive days. The effect of ethanolic extracts of leaf and fruit of T. dioica and leaf of C....
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