Posted by admin on Oct 31, 2018 in |
The objective of the method was to develop a simple, rapid, sensitive, precise, accurate and validated Ultra Performance Liquid Chromatographic (UPLC) method for the simultaneous estimation of Sofosbuvir and Velpatasvir in tablet dosage form. Chromatographic separation was achieved on an acquity UPLC HSS C18, 2.1 × 100 mm, 1.8µ column with a mobile phase composed of orthophosphoric acid buffer and acetonitrile in the ratio of 45:55 at a flow rate of 0.2 ml/min and 1 μl injection volume. The effluents were detected at a wavelength of 250 nm using TUV detector. The retention times of Sofosbuvir and Velpatasvir were found to be 1.425 and 1.767 min respectively. The method was validated with respect to specificity, accuracy, linearity, precision, robustness. The correlation coefficient for Sofosbuvir and Velpatasvir were found to be 0.9992 and 0.9995 respectively. Recovery of Sofosbuvir and Velpatasvir in formulation was found to be 99.48% and 99.6% respectively. Due to simplicity, high precision and rapidness the method can be successfully applied for simultaneous estimation of Sofosbuvir and Velpatasvir...
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Posted by admin on Oct 31, 2018 in |
Sulphur water is commonly used as the remedial source for treating skin infections and eradicating pathogenic microbes causing infection. The sulphur water is the source of sulphur oxidizing bacteria which is the source of different secondary metabolites which has potential killing activity against different skin borne infections. In the present investigation, the study was performed to isolate and screen the sulphur oxidizing bacteria (both autotrophs and heterotrophs) from the sulphur water. Sulphur is primarily used to ease red, itchy skin. It also helps protect your body against toxins in the environment. In addition, people with arthritis may find pain relief from taking a soothing bath in hot sulphur springs. Sulphur aids in certain digestive disorders, especially acid reflux, indigestion made worse by milk, chronic diarrhea and vomiting in the morning. It also helps gynecological problems such as premenstrual syndrome and menopausal discomforts. The total of twenty six isolates were obtained from sulphur as follows: UKAK1, UKAK2, UKAK3, UKAK4, UKAK5, UKAK6, UKAK7, UKAK8, UKAK9, UKAK10, UKAK11, UKAK12, UKAK13, UKAK14, UKAK15,...
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Posted by admin on Oct 31, 2018 in |
A sensitive, accurate, and precise stability-indicating HPTLC method has been developed for the simultaneous estimation of lamivudine (LMV) and dolutegravir sodium (DOL) in bulk and pharmaceutical dosage formulation. The method employed chloroform: methanol: toluene formic acid (8:2:2:0.2 v/v/v/v) as a mobile phase and silica gel G 60 F254 TLC plates as stationary phase. Chromatographic detection was performed at 271 nm. The Rf Value of LMV and DOL was found to be 0.38 ± 0.02 and 0.62 ± 0.02 respectively. The method was validated in compliance with ICH Guideline for linearity, limit of detection (LOD), limit of quantification (LOQ), precision, specificity, accuracy and robustness. The linear regression analysis shown good linear relationship over the concentration range of 120 – 720 ng/spot for LMV (R2 = 0.9994) and 20 – 120 ng /spot for DOL sodium (r2 = 0.999). The LOD of LMV and DOL was found to be 8.86 ng/spot and 2.92 ng/spot respectively. The LOQ of LMV and DOL was found to be 26.5 ng/spot and 8.74 ng/spot respectively....
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Posted by admin on Oct 31, 2018 in |
Randia spinosa (Rubiaceae family), commonly known as ‘Mainphal’ is a large shrub or small tree. The plant grows in Brazil, Ceylon, China, East tropical Africa, India, Malaya and Sumatra. The leaves of Randia spinosa is used in traditional medicine to treat diarrhea and dysentery, snake bite, wound healing, inflammation, tumors, piles, gastrointestinal and skin diseases. The objective of this study is to investigate pharmacognostical, phytochemical features and antioxidant activity of ethanol and aqueous extracts of Randia spinosa leaves by using DPPH assay method. Pharmacognostical characteristics like microscopy, total ash, acid insoluble ash, water insoluble ash and sulphated ash and loss on drying of Randia spinosa leaves were determined. The preliminary phytochemical studies were performed with various reagents and chemicals on leaf extracts in order to determine the various secondary metabolites. The ethanol and aqueous extracts of Randia spinosa leaves were screened for in-vitro antioxidant activity by oxygen radical scavenging such as 1, 1-diphenyl-2- picryl hydrazyl (DPPH) method. Pharmacognostical studies reveal the microscopical and macroscopical characters of Randia spinosa leaves....
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Posted by admin on Oct 31, 2018 in |
Two medicinal plants (Khaya senegalensis and Melia azedarach), frequently used by a large part of the Senegalese population and in particular that of the natural region of Casamance in the traditional treatment of malaria, were selected to study phytochemistry and to compare the antimalarial activity of the different parts used (leaves, bark, and seeds). The extraction of these drugs was carried out successively following a gradient of increasing polarity with cyclohexane, ethyl acetate, dichloromethane, and methanol. In-vitro anti-plasmodial screening of the different fractions was performed on chloroquine-sensitive and chloroquine – resistant strains of Plasmodium falciparum (3D7 strain and W2 strain, respectively). The MDEK fraction is the most active on 3D7 strain with an IC50 = 1.81 ± 0.53 μg / ml (Selectivity index > 55.25). In-vitro cytotoxicity assays on human umbilical vein endothelial cells (HUVEC cells) were performed and the selectivity index was calculated. These tests reveal the non-toxicity of the fractions tested with high CC50 and very often greater than 100 μg /...
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