Posted by admin on Nov 30, 2018 in |
Potassium bromate (KBrO3–) is a chemical compound commonly used as a oxidizing agent that has been used as a food additive for baking of white bread, bun, pav and pizza bread. Potassium bromate (KBrO3–) is mainly known to generate free radicals, which cause oxidative damage to essential cellular macro molecules, leading to cause nephrotoxicity in experimental animals. In this part of experiment biochemical profile i.e. alkaline phosphatase, acid phosphatase and protein content along with ovarian histopathological changes were observed. The animals were treated with KBrO3–, 0.7 mg/g b.wt. in 0.125 ml distilled water orally through canulla upto 15 and 30 days. It has been observed significantly lowered ALP, protein and ACP level elevated. However ovarian histophathological changes were not observed after 15 days KBrO3– treatment. Along with the ovary treated with potassium bromate upto 30 days show degenerative cells in the thecal layer and less amount of cytoplasmic was noticed. Our result conclude that in a very low amount, potassium bromate if is available in the body may pose...
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Posted by admin on Nov 30, 2018 in |
In the present work, a rapid, specific, accurate and precise Reversed phase high performance liquid chromatographic (RP-HPLC) method has been developed and validated for simultaneous determination of Dapagliflozin and Saxagliptin in bulk and pharmaceutical dosage form. Successful chromatographic separation of Dapagliflozin and Saxagliptin was carried out with Inertsil-ODS, C18 column (250 × 4.6 mm; 5 μm) with mobile phase consisted of a mixture of Methanol and Potassium dihyrogen phosphate buffer in the ratio of 45:55 v/v delivered at a flow rate of 1.0 ml/min. The eluents are monitored by PDA detector and peaks values were measured at 210 nm. The retention times for Dapagliflozin and Saxagliptin were 4.707 min and 6.684 min respectively. The present analytical method was validated according to ICH guidelines (ICH, Q2 R1). The linearity study of Dapagliflozin and Saxagliptin was found in the concentration range of 20-70 μg/ml and 20-70 μg/ml respectively and coefficient of variance was 0.999 for both drugs. % recovery was found to be 100.37% and 100.16% for Dapagliflozin and Saxagliptin respectively....
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Posted by admin on Nov 30, 2018 in |
The whole plant extracts of N. crociformis and N. infundibulifolia was analyzed by priliminary phytochemical, total phenolics, flavonoid, carbohydrate and alkaloid content by standard methods. In-vitro antioxidant DPPH assay and Phosphomolybdenum assay developed by Braca et al., Prieto et al., respectively. In-vitro antibacterial (agar well diffusion) activity by gram negative Pseudomonas aeroginosa (ATCC 27853), gram positive (Streptococcus mutans MTCC 890 & Staphylococcus aureus ATCC 25923, Esteritia coli ATCC 25922) and antifungal activity by Aspergillus niger (ATCC 16404). The study shows that the petroleum ether, chloroform, ethanol and aqueous extract of the plants contains a good number of phytochemicals such as flavonoid, cardiac glycoside, saponin, alkaloid etc. as well as antioxidant with respect to the scavenging activity against free radicals DPPH which were roughly comparable to that of BHT. The plant extracts also exhibited potent antimicrobial activity against S. aureus, P. aeruginosa, S. mutans, E. coli and A. niger indicating its bactericidal and fungicidal properties. It may be concluded that the plant Nervilia crociformis is chemically more active than Nervilia...
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Posted by admin on Nov 30, 2018 in |
Microsphere drug delivery systems have used to improve patient compliance, decreases toxicity, and increase efficacy. Also, the use of microspheres to deliver drugs has many other merits, like control-release of the drug, increase bioavailability and target delivery of the drug to the desired site. In this research shows the utility of encapsulating hydroxypropyl methyl cellulose (Methocel K4M) in biodegradable microsphere delivery system, to be delivered orally via a capsule, to gives desired therapeutic action. Microsphere formulations have advantage over conventional tablet or capsule formulations, since it increases the surface area exposed to the absorption site and thus increasing the absorption of the drug and decreasing the dosing frequency of the drug. Flurbiprofen is a non-steroidal anti-inflammatory agent which is used widely in various colon diseases ulcerative colitis, Crohn’s disease, carcinomas and infections. Flurbiprofen shows maximum absorption in the lower gastrointestinal tract regions, and shows half life 4 – 5 h, it shows low bioavailability orally. The microsphere formulations were evaluated for its production yields, actual drug content, encapsulation efficiency,...
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Posted by admin on Nov 30, 2018 in |
Tecoma stans is a well-known traditional plant in India. The plant can be used in diabetes, digestive problems, and rheumatism and as antimicrobial. The aim of the study is to evaluate the anti-inflammatory activity of extracts of the stem bark of Tecoma stans (L.) by in-vitro and in-vivo methods. Ethyl acetate and alcoholic extract showed better anti-inflammatory activity by Human red blood cell membrane stabilization method. These extracts were also found to have significant inhibition to the carrageenan induced rat paw oedema. The gallic acid, a phenolic acid identified in the ethyl acetate fraction may contribute to the anti-inflammatory activity. The amount of gallic acid present in the ethyl acetate fraction of Tecoma stans (L.) was found to be 1.021 ng/mg extract, by HPTLC. The study concludes that the anti-inflammatory activity of stem bark of Tecoma stans may be due to the phenolics present in the extracts. Further studies are suggested to isolate and identify the more components responsible for the...
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