Posted by admin on Jan 31, 2019 in |
The rhizomes of Curcuma caesia Roxb. (Zingiberaceae) Are traditionally used in the treatment of various ailments and metabolic disorders like leucoderma, asthma, tumors, piles, bronchitis, etc. in Indian system of medicine. Considering the importance of natural products in modern phytomedicine, the antioxidant and ant mutagenic activities of C. caesia Roxb. rhizome extract and its fractions were evaluated. The antioxidant results indicate that the free radical-scavenging activity may be attributed to the high contents of phenolics and flavonoids with a higher reducing capacity. The IC50 values of in-vitro and ex-vivo antioxidant activities of Curcuma caesia was found for DPPH scavenging activity was 1.08 ± 0.06 μg/ml, nitric oxide scavenging activity was 37.50 ± 1.54 μg/ml, Hydroxyl radical scavenging activity was 10.08 ± 2.01 μg/ml, Superoxide Anion scavenging activity was 29.63 ± 2.07 μg/ml respectively. The in-vitro antimicrobial activity of different fractions obtained from the rhizome of Curcuma caesia was investigated against standard strain and clinical isolates of Staphylococcus aureus. The clinical isolates were found more sensitive for different fractions, than...
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Posted by admin on Jan 31, 2019 in |
Cardiovascular diseases (CVDs) cause 30% of deaths globally and are reported to be on the increase in sub-Saharan Africa. Medications prescribed for a CVD require a lifetime commitment. As a result patient must adhere to prescribed medication. However, prescription adherence is influenced to a large part by prescription knowledge. Although disease outcome is influenced by prescription knowledge, estimates of prescription knowledge and of factors that influence it are largely unknown in developing countries such as Kenya. The goal of the present study was to investigate factors that influence the level of prescription knowledge among patients with CVD at the Moi Teaching and Referral Hospital (MTRH). Specifically, the study determined the level of prescription knowledge, the association between socio-demographic characteristics of patients and their prescription knowledge, and whether prescribing clinicians explain prescriptions given to patients with CVD. The study used a cross-sectional study design with a sample size of 204. Data were collected using questionnaires. There was a significant difference (X² = 144.75, p = 0.000) in the frequencies between...
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Posted by admin on Jan 31, 2019 in |
We here in the report the investigation of the antifungal activity of various solvent extracts obtained from a marine brown alga, Lobophora variegata against three fungal dermatophytes, namely Candida albicans, Candida tropicalis, Trichophyton mentagrophytes and a nondermatophyte Aspergillus flavus using disc diffusion method. Among the solvent extracts tested in the investigation, only the methanol extract of the L. variegata exhibited better antifungal activity against all the fungi and chloroform extract was able to elicit activity against C. albicans and T. mentagarophytes. On the other hand, the extract of the L. variegata obtained from ethyl acetate was active against C. albicans only. In contrast to the methanol and chloroform extracts, the aqueous and hexane extracts of the L. variegata were found to be least effective and showed no observable activity against any of the test fungi. The investigation demonstrated that the methanol extract of brown alga L. variegata is more effective on dermatophyte...
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Posted by admin on Jan 31, 2019 in |
The study was carried out to evaluate anti-inflammatory activity of alcoholic extract of roots of Lawsonia Inermis (LILAC). In-vitro anti-inflammatory activity was evaluated by Cyclooxygenase inhibitory assay wherein the LIALC showed a dose-dependent inhibition of both the enzymes with IC50 values of 58.2 μg/ml for COX-1 and 7.38 μg/ml for COX-2. In RBC membrane stabilization activity, LILAC at the concentration of 100 µg/ml and 200µg/ml exhibited membrane stabilizing activity. Acute oral toxicity of extract was determined by OECD 423 guidelines wherein mortality was not observed when the extract was administered orally up to 2000 mg/kg. Carrageenan-induced paw edema model evaluated in-vivo anti-inflammatory activity; Paw edema was induced by injecting 0.1 ml of 1% w/v carrageenan into the subplantar tissues of the left hind paw of each rat. LILAC at all the three doses, 200, 300 and 400 mg/kg, showed maximum inhibition of 31.8, 40.9 and 50.8% respectively at 180 min. In Cotton pellet-induced granuloma model, Sterile, weighed, cotton pellets (10 ± 1 mg) were implanted subcutaneously in the groin...
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Posted by admin on Jan 31, 2019 in |
The several spectrophotometric and HPLC methods have been reported for determination of Nebivolol and Valsartan individually or in combination with other drugs in pharmaceutical dosage forms. Hence, in the present study, a new, sensitive, suitable and cost-effective Reversed-Phase High-Performance Liquid Chromatography method was developed and validated for the determination of Nebivolol and Valsartan in bulk and tablet formulation. In RP-HPLC method, the analyte was resolved by using a gradient system, methanol and water OPA 0.1% (70:30 v/v) pH 3.5 was used as mobile phase, at a flow rate of 0.7 ml/min, on HPLC system containing UV- detector with Workstation Software and Greece C18 column (4.6 × 250 mm; 5μm). The detection was carried out at 274 nm. The retention time was found to be 5.0500 min and 9.7167 min for Nebivolol hydrochloride and Valsartan respectively. The method gave the good resolution and suitable retention time. The results of the analysis in the method were validated regarding accuracy, precision, specificity, linearity, limit of detection, limit of quantification and robustness. A...
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