Posted by admin on Nov 30, 2018 in |
Sickle cell anaemia is a genetic blood disorder that is caused by a mutation in the gene that encoded b-globin of haemoglobin, which results as the presence of abnormal haemoglobin HbS in the RBC. Presently, there is no universal cure for sickle cell anaemia Herbomineral formulations were standardized for organoleptic properties (colour, odour and taste), physiochemical properties, total ash value, moisture content, pH of the solution, to find effective agent in which we investigated the antisickling potential of T-AYU- HM Premium, a novel Herbomineral formulation. In-vitro sickling of homozygous sickle cells upon deoxygenation (Emmel’s Test) showed dose dependent inhibition of sickling in presence. T-AYU-H and T-AYU-HM Premium (25 µg/ml, 50 µg/ml, 100 µg/ml and 500 µg/ml). Mechanistic evaluations were performed such as, t-BOOH induced oxidative haemolysis, and formation of met-haemoglobin was significantly decreased in dose dependent manner in the presence of 50 µg/ml, 100 µg/ml and 500 µg/ml of T-AYU-H and T-AYU-HM Premium. Membrane stabilization activity by osmotic fragility test showed significant dose dependent increased stabilization in the presence...
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Posted by admin on Nov 30, 2018 in |
Ultraviolet radiation (UV) from sun has significant detoriating and degenerative effects on human skin and thus responsible for much skin disease. The most authentic and realistic approach to overcome this issue is to use or apply a barrier between sun rays and exposed skin surface using suitable products called sunscreens or UV protectors. Sunscreens usually include both natural and synthetic molecule and have great application in cosmaceuticals. In the present study one such natural product-rose water was evaluated for sun protecting ability of the rose water samples (SPF) using in-vitro spectroscopic method. A correlation was also established by evaluating the antioxidant activity in total 12 samples of rose water were evaluated for SPF and antioxidant activity Among all the samples the SPF value of sample #09 showed high sunscreen activity 3.956 followed by sample #08 with SPF value of 3.612. The rose water sample distilled in the lab indicated SPF value as 0.800. All the samples showed antioxidant activity (free radical scavenging ability). The phytochemical investigation indicates the presence...
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Posted by admin on Nov 30, 2018 in |
Antitumor and antioxidative efficacy of Carissa carandas extract (CCE) was investigated in this study against skin carcinogenesis which was initiated by a single topical application of 7, 12-dimethyl-benz(a)anthracene (100 mg / 100 ml acetone in 1% acetone) and two weeks, later promoted by repeated treatment of TPA (Croton oil) for 16 weeks (in 1% acetone) in Swiss albino mice. Oral administration of Carissa carandas hydroalcholic extract (100 mg/kg/b.wt/day) during the peri-initiational (i.e. 7 days before and 7 days after the application of DMBA) and post-initiational (i.e. from the day of croton oil treatment and continued till the end of the experiment) stage and exhibited a reduced number of tumor burden, tumor yield, number of tumors and percent incidence of mice bearing skin tumors as compared to DMBA-TPA treatment group. Average latent period and inhibition of tumor multiplicity were increased when compared to positive control values (only DMBA-TPA treated mice). After oral administration of CCE histopathological changes found less as compared to carcinogen treated mice in both the Groups. Thus,...
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Posted by admin on Nov 30, 2018 in |
Plasmodium falciparum and multidrug resistant bacteria such as Mycobacterium avium, Staphylococcus aureus and Escherichia coli are among the top causes of morbidity and mortality especially in developing countries. These pathogens are responsible for the reported antibiotic resistance incidents globally. Due to the increase in antibiotic resistance worldwide, there is an urgent need for new bioactive compounds. This study aimed at determining the antiplasmodial and antimicrobial activity of secondary metabolites produced by soil actinomycetes. Soil samples were collected from Kavango and Hardap regions and cultured on various media to isolate and identify actinomycetes. Secondary metabolites produced were extracted using different solvents. The antimicrobial activity of different isolated secondary metabolites was determined against M. avium, S. aureus and E. coli. Antiplasmodial activity of four bioactive fractions with potent antibacterial activity was further determined against a chloroquine resistant (D10) P. falciparum strain at various concentrations. Bioactive compounds from two isolates displayed activity against M. avium, whilst another two showed activity against E. coli. None of the secondary metabolites displayed activity against S....
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Posted by admin on Nov 30, 2018 in |
The biologically active compounds in plants reckoned their application in medicines from ancient times. Among the biologically active compounds enzymes have evolved as a potential therapeutic agent. L-asparaginase a therapeutic protein is extensively employed in medical sector to diagnose and treat leukemia. Bacteria, fungi, yeast and plants have been identified as potential sources of L-asparaginase. The side effects associated with microbial L-asparaginase restricted their utilization in treatment of leukemia and bought medicinal plants as source into focus. In the present study, different species of Phyllanthus genera have been screened for presence of L-asparaginase in different seasons. The leaves of Phyllanthus emblica with highest enzyme activity (20.3 IU/ml) and specific activity (5.2 IU/mg) emerged out as potential source for L-asparaginase production. Phyllanthus amarus and Phyllanthus niruri also contained appreciable amount of L-asparaginase. This is the first report of identifying presence of L-asparaginase in Phyllanthus genera. L-asparaginase from Phyllanthus emblica was further purified to homogeneity and the molecular mass of enzyme was found to approximately 85kDa. The yield of purified enzyme...
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