Posted by admin on Nov 30, 2018 in |
Lamotrigine (LTG), a synthetic anticonvulsant, may lead to therapy failure or toxicity risks due to the wide inter and intra individual pharmacokinetic variations. Those wide variations in LTG require its therapeutic drug monitoring (TDM). Our study aims to compare the QMS method with the HPLC-UV which is considered as a reference method for TDM of LTG. The study was conducted in the Clinical Pharmacology Department of the National Centre of Pharmacovigilance during a period of nine months. It included 37 samples from 37 patients treated with LTG. The samples were monitored by HPLC-UV and by PETIA. Regression analysis performed with data yielded to the equation LTGPETIA = 0.7701 LTGHPLC + 1.475 with a Pearson correlation r = 0.804 (P<0.0001). Bias mean between the two methods was 13.45%. In conclusion, the turbidometric technique (QMS) is comparable to the HPLC-UV method in the interval of therapeutic concentration. Under low concentrations, the QMS technique is less...
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Posted by admin on Nov 30, 2018 in |
Antimicrobial activity of 11 ethnomedicinal plant extracts was evaluated against five bacterial strains (Escherichia coli, Staphylococcus aureus, Bacillus subtilis, Pseudomonas aeruginosa, Proteus vulgaris) and one fungal strain (Candida albicans). Plants were collected from different regions of Kashmir Valley and the ethnomedicinal data were gathered from traditional healers and elders of the study area. Cold percolation method was used to obtain hexane and methanol extracts and the antimicrobial activity was found using paper disc diffusion method. The results indicated that all plants, exhibited antimicrobial activity against one or more of the tested microorganisms at three different concentrations of 1.25, 2.5 and 5 mg/disc. Among the plants tested, Nepeta cataria, Allium consangium, Artemisia biensis and Ocimum sanctum were most active. The highest antifungal activity was exhibited by hexane extract of Nepeta catariaagainst Candida albicans. Compared to hexane extract, methanol extract showed significant activity against tested organisms. This study also showed that Nepeta cataria, Allium consangium, Artemisia biensis and Ocimum sanctum could be potential sources of new antimicrobial agents. Among the tested...
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Posted by admin on Nov 30, 2018 in |
Evaluation of different natural and synthetic polymers as protective layer polymers in the manufacturing of control release multi-unit pellet tablets, Tolterodine Tartrate was selected as a model drug with aqueous ethylcellulose suspension is used as control release polymer. Tolterodine Tartrate is highly soluble BCS Class- I molecule, hence selected aqueous solution layering method for drug loading in Fluid bed processor, Optimized formulation was manufactured by using seal coating on microcrystalline cellulose pellets followed by drug loading and control release coating applied by using solution and suspension layering method respectively in Fluid bed processor. Given coating on these functional coated pellets with different natural and synthetic polymers like Klucel, Polyethylene glycol 6000, Hypromellose 5 cps, Guar gum, and Xanthan gum. Evaluated these pellets for physical characterization, DSC, SEM and comparative in-vitro dissolution profiles. Drug release profiles of Control release MUPS tablets containing protective layer coating was compared to those control release pellets and f2 values observed was more than 50 with Klucel, Polyethylene glycol 6000, Hypromellose 5 cps, Guar gum,...
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Posted by admin on Nov 30, 2018 in |
In the present work the nanoliposomes prepared by using REV and MEU methods were coated with different concentration of chitosan solutions. The main objective behind this work was to assess the physical properties, stability and in vitro release of uncoated and chitosan coated nanoliposomes of gefitinib. The existence of a covering on the exterior of nanoliposomes was confirmed by the modification in particle size and zeta potential. The chitosan coated nanoliposomes had shown significantly slow in-vitro drug release than uncoated nanoliposomes that confirmed the increased stability of nanoliposomes. The transmission electron microscopy, particle size analysis, FTIR studies, DSC analysis, and zeta potential studies were used to investigate the characteristics of uncoated and chitosan-coated nanoliposomes to develop and further optimize nanoliposomes that are directed for their systemic pharmacological applications. Stability studies and cytotoxicity studies of uncoated and chitosan coated nanoliposomes were performed. Chitosan coated formulations were found to be more stable and more cytotoxic than uncoated formulations. The ideal temperature was found to be 4...
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Posted by admin on Nov 30, 2018 in |
Disorders caused by excessive use of alcohol known as alcohol use disorders (AUD) are the most common cause of liver diseases including cirrhosis. In adults, the most common cause of death is alcohol consumption which leads to an array of alcoholic liver diseases (ALD). Present study has been conducted to investigate the hepatoprotective activity of Bixa orellana L. (BO) seed extract against acute ethanol-induced hepatotoxicity in rats. The characterization of BO seed extract was performed using standard biochemical analysis. 50% ethanolic extract of Bixa orellana (BOE 200 and 400 mg/kg body weight) was administered daily for 8 days in experimental animals for the assessment of hepatoprotective activity. To develop hepatotoxicity, animals were orally administered with alcohol (40%) 12 ml/kg at 2 h after the doses of BOE every day for eight consecutive days except the rats of normal group. The hepatoprotective activity was assessed using various biochemical parameters like aspartate aminotransferase, alanine aminotransferase, alkaline phosphatise, bilirubin, albumin, cholesterol and lactate dehydrogenase. However, the results demonstrated that the treatment with...
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