Posted by admin on Sep 30, 2018 in |
ABSTRACT: Fibre rich pasta were prepared by substituting refined wheat flour with carrot pomace powder (CPP) at 5%, 15%, and 25% and evaluated for its physical properties, chemical composition, textural properties and sensory characteristics. The fat content of the pasta showed no pronounced variation. The hardness of the pasta increased with increase in the level of incorporation of CPP in the flour blends. CPP improved the appearance of pasta by imparting it attractive colour; however the texture score decreased with the increase in the level of supplementation. Pasta with 15% CPP was found to be most acceptable due to attractive appearance; and better taste and...
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Posted by admin on Sep 30, 2018 in |
The objective of the present study is to develop a pharmaceutically stable sustained release matrix tablets of Baclofen and perform the pre-compression, post compression and in-vitro evaluation studies of developed formulation. In this study sustained release matrix tablets of Baclofen were prepared by wet granulation method using mannitol, HPMC K4M, sodium alginate, HPMC K15 in various concentrations. All the formulations have showed acceptable Pharmacopeial standards. Formulation F9 have extended the release of Baclofen upto 12 h. Model fitting analysis for formulation F9 fitted in the zero order model and korsemeyer- peppas model. The “n” values obtained from the peppas-korsemeyer equation suggested that, drug release was non-fickian diffusion mechanism. Successful formulation was found stable after evaluation for physicochemical parameters when kept for 30 days at room temperature, 40 ºC and 2-8...
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Posted by admin on Sep 30, 2018 in |
The objective of the present work is to develop orodispersible tablets of Metformin hydrochloride using agar as natural super disintegrant to improve bioavailability, disintegration time, dissolution efficacy and patient compliance. Fast dissolving drug delivery system has numerous advantages over conventional dosage form like tablet, capsule, syrups etc. Formulation of nine batches out of which batch F5 with 6% super disintegrant was found to have better results in compare to other formulations, the F5 batch was passed with friability test and found negligible loss (0.4%), Then the batch was further evaluated for disintegration test and found to have 11.03 sec and in-vitro dispersion was found to be 15 sec in simulated saliva fluid and percentage drug release was evaluated to be 98.5% in less than 30 min. Hence, F5 batch was found to be better as it contain 6% treated agar. It shows further potential to carry animal...
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Posted by admin on Sep 30, 2018 in |
Globally, colorectal cancer is the third most common known today. Lack of cheap, rapid, safe and reliable cancer treatments resulted to the continued pursuit to find natural sources of bioactive compounds that can be cytotoxic or damaging to the DNA of cancer cells. One of the potential resource is the edible oyster mushroom (Pleurotus ostreatus var Florida) reported to have medicinal properties. Since many of the studies did not show specific varieties of the species used for the evaluation specifically on the anti-tumor and anticancer properties of the species, this study was therefore conducted. To determine whether there are cytotoxic and genotoxic effects of the ethanolic extracts of this variety of the white oyster mushroom against HT-29 colon cancer cell lines by damaging the DNA of cancer cells, the Comet assay method was used. This assay made use of differences in the measurements calculated by Open Comet Software of the tail length, tail DNA, tail DNA percent and tail moment in untreated and IC50 ethanolic extract-treated cells. Results of...
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Posted by admin on Sep 30, 2018 in |
L-asparaginase has been accepted clinically as an anti-tumour agent for the effective treatment of acute lymphoblastic leukaemia and lymphosarcoma. This enzyme also possesses L-glutaminase activity and causes immunological problems. Hence, efforts have been made to develop mutants with lower or no glutaminase activity. In the present study, a homology model of L-asparaginase obtained from Pectobacterium carotovorum was docked and compared with its mutants for activities, analysed for molecular dynamics and structural stability. A total of five in-silico mutants were developed using single amino acid mutagenesis and evaluated for ligand binding for both L-asparagine and L-glutamine. One of the mutants, Y306L, showed -5.89 and -5.04 while the wild L-asparaginase revealed -5.50 and -5.12 binding energies for L-asparagine and L-glutamine, respectively. Further, substrate-enzyme interaction analysis indicated that the wild L-asparaginase showed five interactions for L-asparagine as well as for L-glutamine, whereas, the mutant, Y306L depicted eight and three interactions for L-asparagine and L-glutamine. Molecular dynamics analysis denoted that mutant protein is more stable in RMSD, RMSF and radius of gyration to...
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