Posted by admin on Oct 31, 2018 in |
The present study is focused on the electrochemical behavior of glycine 4-bromo acetophenonimine (GBAPI) and its Ni(II) complex. In buffer solutions (Britton-Robinson universal buffer and phosphate buffer) of pH 3 – 9 range, using cyclic voltammetric technique. GBAPI is synthesized by the condensation of 4-bromo acetophenone and glycine amino acid (1:1 molar ratio). The complex is generated by the reaction of nickel (II) acetate and GBAPI in 1: 2 molar ratio in reflexing alcohol. The process is irreversible and diffusion controlled for both GBAPI and Ni(II) complex. The effects of change in pH, solvent and sweep rate are evaluated. Kinetic parameters (charge transfer coefficient (αn), diffusion coefficient (D01/2), rate constant (k°f,h) are calculated from cyclic voltammetric measurements. These compounds are screened for their antibacterial and antifungal activities and the results are compared with those of a standard antibacterial and antifungal...
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Posted by admin on Oct 31, 2018 in |
The leaves of crepe ginger (Costus speciosus (J. Koeng.) Sm. are utilized by the Subanen tribe of Lapuyan, Zamboanga del Norte, Philippines to treat diabetes. However, the hypoglycemic potential of this plant is not yet well-established. Thus, the present study investigated the in-vitro alpha-amylase inhibitory and antioxidant activities of the leaves of C. speciosus. The evaluation of the alpha-amylase inhibitory activity was conducted in-vitro using soluble starch. The 2, 2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity was employed in the evaluation of antioxidant activity. Total phenolics content was investigated using Folin-Ciocalteu reagent. The presence of phytoactive compounds that might contribute to its activities was determined through phytochemical screening and Gas chromatography – Mass spectrometric analysis. The results revealed that the leaf extracts exhibited alpha-amylase inhibitory potential and antioxidant activity which might be due to the presence of bioactive compounds such as eicosane, nonadecane, squalene, phenol, 2, 4-bis (1, 1-dimethylethyl), d-limonene, and hexadecanoic acid, ethyl ester. Negative and low correlation between DPPH scavenging activity and total phenolics content were obtained from both...
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Posted by admin on Oct 31, 2018 in |
Simple, rapid and reproducible stability-indicating methods were established for quantitative determination of azathioprine using a, phenomenex 250 mm × 4.9 mm C18, 5 µm, inertsil and UV detection at 240 nm. The isocratic elution was used to quantify the analyte and the mobile phase was acetate buffer: acetonitrile: methanol (30: 35: 35) was pumped at 1.0 mL/min. The method was linear between 10-300 µg/mL, statistically validated for its linearity, precision and accuracy. In this study, degradation behavior of azathioprine was studied by subjecting the drug to various ICH stress conditions. The intra and inter day variation was found to be less than 1% showing high precision of the assay method. It was found that the excipients in the commercial tablet did not interfere with the method. Developed method can routinely use for the estimation of azathioprine related compounds from the dosage form and also for stability...
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Posted by admin on Oct 31, 2018 in |
The objective of the method was to develop a simple, rapid, sensitive, precise, accurate and validated Ultra Performance Liquid Chromatographic (UPLC) method for the simultaneous estimation of Sofosbuvir and Velpatasvir in tablet dosage form. Chromatographic separation was achieved on an acquity UPLC HSS C18, 2.1 × 100 mm, 1.8µ column with a mobile phase composed of orthophosphoric acid buffer and acetonitrile in the ratio of 45:55 at a flow rate of 0.2 ml/min and 1 μl injection volume. The effluents were detected at a wavelength of 250 nm using TUV detector. The retention times of Sofosbuvir and Velpatasvir were found to be 1.425 and 1.767 min respectively. The method was validated with respect to specificity, accuracy, linearity, precision, robustness. The correlation coefficient for Sofosbuvir and Velpatasvir were found to be 0.9992 and 0.9995 respectively. Recovery of Sofosbuvir and Velpatasvir in formulation was found to be 99.48% and 99.6% respectively. Due to simplicity, high precision and rapidness the method can be successfully applied for simultaneous estimation of Sofosbuvir and Velpatasvir...
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Posted by admin on Oct 31, 2018 in |
Sulphur water is commonly used as the remedial source for treating skin infections and eradicating pathogenic microbes causing infection. The sulphur water is the source of sulphur oxidizing bacteria which is the source of different secondary metabolites which has potential killing activity against different skin borne infections. In the present investigation, the study was performed to isolate and screen the sulphur oxidizing bacteria (both autotrophs and heterotrophs) from the sulphur water. Sulphur is primarily used to ease red, itchy skin. It also helps protect your body against toxins in the environment. In addition, people with arthritis may find pain relief from taking a soothing bath in hot sulphur springs. Sulphur aids in certain digestive disorders, especially acid reflux, indigestion made worse by milk, chronic diarrhea and vomiting in the morning. It also helps gynecological problems such as premenstrual syndrome and menopausal discomforts. The total of twenty six isolates were obtained from sulphur as follows: UKAK1, UKAK2, UKAK3, UKAK4, UKAK5, UKAK6, UKAK7, UKAK8, UKAK9, UKAK10, UKAK11, UKAK12, UKAK13, UKAK14, UKAK15,...
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