Posted by admin on Oct 31, 2018 in |
A sensitive, accurate, and precise stability-indicating HPTLC method has been developed for the simultaneous estimation of lamivudine (LMV) and dolutegravir sodium (DOL) in bulk and pharmaceutical dosage formulation. The method employed chloroform: methanol: toluene formic acid (8:2:2:0.2 v/v/v/v) as a mobile phase and silica gel G 60 F254 TLC plates as stationary phase. Chromatographic detection was performed at 271 nm. The Rf Value of LMV and DOL was found to be 0.38 ± 0.02 and 0.62 ± 0.02 respectively. The method was validated in compliance with ICH Guideline for linearity, limit of detection (LOD), limit of quantification (LOQ), precision, specificity, accuracy and robustness. The linear regression analysis shown good linear relationship over the concentration range of 120 – 720 ng/spot for LMV (R2 = 0.9994) and 20 – 120 ng /spot for DOL sodium (r2 = 0.999). The LOD of LMV and DOL was found to be 8.86 ng/spot and 2.92 ng/spot respectively. The LOQ of LMV and DOL was found to be 26.5 ng/spot and 8.74 ng/spot respectively....
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Posted by admin on Oct 31, 2018 in |
Randia spinosa (Rubiaceae family), commonly known as ‘Mainphal’ is a large shrub or small tree. The plant grows in Brazil, Ceylon, China, East tropical Africa, India, Malaya and Sumatra. The leaves of Randia spinosa is used in traditional medicine to treat diarrhea and dysentery, snake bite, wound healing, inflammation, tumors, piles, gastrointestinal and skin diseases. The objective of this study is to investigate pharmacognostical, phytochemical features and antioxidant activity of ethanol and aqueous extracts of Randia spinosa leaves by using DPPH assay method. Pharmacognostical characteristics like microscopy, total ash, acid insoluble ash, water insoluble ash and sulphated ash and loss on drying of Randia spinosa leaves were determined. The preliminary phytochemical studies were performed with various reagents and chemicals on leaf extracts in order to determine the various secondary metabolites. The ethanol and aqueous extracts of Randia spinosa leaves were screened for in-vitro antioxidant activity by oxygen radical scavenging such as 1, 1-diphenyl-2- picryl hydrazyl (DPPH) method. Pharmacognostical studies reveal the microscopical and macroscopical characters of Randia spinosa leaves....
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Posted by admin on Oct 31, 2018 in |
Two medicinal plants (Khaya senegalensis and Melia azedarach), frequently used by a large part of the Senegalese population and in particular that of the natural region of Casamance in the traditional treatment of malaria, were selected to study phytochemistry and to compare the antimalarial activity of the different parts used (leaves, bark, and seeds). The extraction of these drugs was carried out successively following a gradient of increasing polarity with cyclohexane, ethyl acetate, dichloromethane, and methanol. In-vitro anti-plasmodial screening of the different fractions was performed on chloroquine-sensitive and chloroquine – resistant strains of Plasmodium falciparum (3D7 strain and W2 strain, respectively). The MDEK fraction is the most active on 3D7 strain with an IC50 = 1.81 ± 0.53 μg / ml (Selectivity index > 55.25). In-vitro cytotoxicity assays on human umbilical vein endothelial cells (HUVEC cells) were performed and the selectivity index was calculated. These tests reveal the non-toxicity of the fractions tested with high CC50 and very often greater than 100 μg /...
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Posted by admin on Oct 31, 2018 in |
Aim: To assess whether Tranexamic acid has any effect in inhibiting degradability of PRF membrane and also to compare the degradability of PRF at different concentrations of Tranexamic acid (200 mg, 150 mg and 50 mg). Material and Methods: The degradability of PRF membrane was compared between various concentrations of Tranexamic acid such as 200 mg, 150 mg, 50 mg against negative control of phosphate buffer solution (PBS). Group 1-PBS (negative control) n = 5, Group 2-PBS containing 200 mg of Tranexamic acid n = 5, Group 3-PBS containing 150 mg of Tranexamic acid n = 5, and Group 4-PBS containing 50 mg of Tranexamic acid n =5. PRF procured from each donor was weighed in a micro weighing machine and they were later dropped in each of the 4 groups of eppendorfs (5 in each group). After 1 week of storing in room temperature, the PRF pieces were retrieved and percentage of remaining PRF was calculated. ANOVA and post hoc tests were used to statistically compared this data...
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Posted by admin on Oct 31, 2018 in |
Background: Dashamoola is a group of ten drugs i.e. Bilva (Aegle marmelos (L.) Corrêa), Patala (Stereospermum suaveolens (Roxb.) DC.), Agnimantha (Clerodendrum phlomidis L.f.), Shyonaka (Oroxylum indicum (L.) Benth. ex Kurz), Gambhari (Gmelina arborea Roxb.), Bruhati (Solanum nigrum L.), Gokshura (Tribulus terrestris L.), Kantakari (Solanum xanthocarpum Schrad. & H. Wendl.), Prishniparni (Uraria picta (Jacq.) DC.), Shalaparni (Desmodium gangeticum (L.) DC.) part used are moola of all the drugs. Dashamoola kwatha choorna is made use for parisheka in the management of spastic cerebral palsy in children. Aim: To standardize Dashamoola kwatha choorna. Materials and Methods: Physico-chemical studies like Loss on drying, acid insoluble ash, total ash, water soluble ash, alcohol soluble extractive, water soluble extractive and HPTLC were carried out as per the WHO guidelines, Indian Pharmacopoeia and Ayurvedic Pharmacopoeia. Conclusion: Standar-dization tests done on Dashamoola kwatha choorna helped in authenticating and ensuring the quality of the...
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