Posted by admin on Sep 30, 2018 in |
The optimized microparticles of Verapamil hydrochloride entrapped in Poly(lactide-co-glycolide) (Verapamil HCl-PLGA) was prepared through solvent displacement method and lyophilization. The optimization parameters for the formulation include particle size, polydispersity index, zeta potential, and entrapment efficiency. The final product was further characterized based on percent particle recovery, redispersibility, percent drug loading, drug release kinetics, and morphology. Increasing the PLGA 75:25 concentrations resulted to an increase in the particles size, polydispersity index and entrapment efficiency, and a decrease in zeta potential. The increase in poloxamer 188 concentration led to a decrease in zeta potential and an increase in the entrapment of the drug. Lastly, the increase in the pH of the non-solvent phase resulted to an increase in particle size. The addition of sucrose led to an unfavorable increase in the particle size and polydispersity index and a decrease in zeta potential and entrapment efficiency after lyophilization. The final product of the process was a heterogeneous sized (<10µm) irregularly shaped particles with an acceptable particle recovery, redispersibility, and percentage drug loading,...
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Posted by admin on Sep 30, 2018 in |
Cyamopsis tetragonoloba is widely used by people and is found to have multiple medicinal utilities. Four (chloroform, ethyl acetate, methanolic and aqueous) extracts of Cyamopsis tetragonoloba fruit were therefore, analysed by Gas Liquid Chromatography and Mass Spectrometry analysis to assess their phytochemical constituents. The chloroform extract of Cyamopsis tetragonoloba fruits showed the presence of thirty one phytochemical constituents of which the major phytochemical compounds were Tetracontane and Stigmasterol. The ethyl acetate extract of Cyamopsis tetragonoloba fruit showed the presence of fifty phytochemical constituents. The important phytochemical compounds were stigmasterol and 4-tert-butylcalix [4] arene. Thirty eight phytochemicals were reported in the methanolic extract. The most important ones in term of peak area and retention time were mome inositol and 7-tetradecenal (Z). Mome inositol and 10, 12- Hexadecadien-1-ol was the primary components in a total of 30 phytochemicals obtained from the aqueous...
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Posted by admin on Sep 30, 2018 in |
The aim of the study was to develop an easy, sensible and rapid method for the estimation of amikacin in both pure and marketed formulation by pre derivatisation technique using reverse phase C18 column in HPLC. In this RP-HPLC method Shimadzu LC 20 AD of PDA detector was used with LC solutions software. In this method gradient elution with a mobile phase of acetonitrile and acetate buffer in the ratio of 25:75 v/v at the flow rate of 2 mL/min and run time of 10 min were used. Validation parameters of HPLC were found at detection wavelength of 272 nm. Linearity was observed with a concentration range of 10 – 50 µg/mL with R2 = 0.99. The method was robust with wavelength 271 and 273 nm with a flow rate of 1.8 and 2.2 mL/min showed good results. The retention time of drug was found at 8.9 min and assay showed 98.1%. RP-HPLC method was validated as per ICH guidelines and can be used for routine quantitative analysis of...
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Posted by admin on Sep 30, 2018 in |
A simple and rapid stability indicating HPLC method for acotiamide hydrochloride was developed and validated. The stationary phase used was thermo gold cyno (250 × 4.6 mm) 5 μ with a mobile phase consisting of mixture of acetonitrile and 10 mM KH2PO4 in water in the ratio of 80:20 v/v at a flow rate of 0.8ml/min. Detection was carried at 284 nm. The retention time observed was 8.1 ± 0.2 min. The column was maintained at ambient temperature and 20 μl of solutions were injected. The eluted compound was detected by using PDA detector. The linear regression analysis data for calibration plot show good relationship with coefficient of regression value, r2=0.99 in the concentration range 10-50 μg/ml. The stress degradation studies were performed as per ICH guidelines. Acotiamide hydrochloride degraded with acid/base hydrolysis, thermal, oxidation and photolytic stress. The method was validated as per ICH Q2A (R1) guideline with respect to linearity, accuracy, precision, specificity and robustness. This method can be used for monitoring the stability of acotiamide...
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Posted by admin on Sep 30, 2018 in |
The objective of the study was to develop mesoporous silica nanoparticles for the poorly water soluble drug ritonavir (RTV) for enhancement of in-vitro dissolution and corresponding in-vivo bioavailability. A comparative assessment between 2D-hexagonal silica nano-structured MCM -41NPs and 3D cubic pore system MCM -48NPs on drug release rate was also investigated. RTV (BCS class II drug), was loaded in the synthesized MCM-41NPs and MCM-48NPs by the solvent evaporation technique. The obtained MCM-41NPs, MCM-48NPs and RTV loaded mesoporous nanoparticles were characterized by different analytical techniques like UV spectrophotometry, differential scanning calorimetry, thermogravimetric analysis, FTIR, N2 adsorption-desorption technique, scanning electron microscopy (SEM), transmission electron microscopy (TEM) and powder-XRD. The in-vitro release profile of RTV was studied in 900 mL 0.1N hydrochloric acid (HCl) medium using USP apparatus-II at 50 rpm. Further; In-vivo studies were performed in Wistar rats where drug loaded mesoporous nanoparticles were compared with pure RTV. In dissolution study MCM-48NPs showed better and fast release of RTV than the MCM-41NPs. In pharmacokinetics study, the maximum peak plasma concentrations of...
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