Posted by admin on Oct 31, 2018 in |
Objective: To detect the prevalence of Plasmid resistant AmpC β-lactamases gene in clinical isolates of gram negative organisms from UTI patients which produce resistance against multiple antibiotics. The gene coding for AmpC β-lactamases is also present in E. coli & Klebsiella species was not expressed because due to lack of promoter region, but the transfer of chromosomal genes to plasmids allows the expression of AmpC β-lactamases that hydrolyze the β-lactam ring, which has greater impact on resistance against multi-drug antibiotics, is a significant problem around the world. Methods: Among 20 non-repetitive clinical isolate of each Escherichia coli and Klebsiella pneumoniae, examined for identification and characterization of urine cultures based on morphological, biochemical tests, antibiotic resistant pattern, modified-disc method and detection of AmpC gene by plasmid identification by agarose gel electrophoresis and amplification of AmpC gene by PCR techniques. Results: The study detects the prevalence of AmpC gene primarily by modified disc inducer method as well as conformational molecular analysis by PCR amplification techniques. AmpC prevalence was observed in both...
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Posted by admin on Oct 31, 2018 in |
Objective: Pyrostegia venusta (Ker Gawl.) Miers, Bignoniaceae, is native to the Brazilian Cerrado and popularly known as “cipó-de-são-joão”. In Brazilian folk medicine, the flowers of P. venusta are used as a general tonic and a treatment for diarrhea, vitiligo, cough, and common infections and inflammatory diseases of the respiratory system. Nevertheless, there are still no studies on its possible anti-inflammatory and analgesic effects. Methods: The P. venusta methanolic and pet. ether extract was used to evaluate the anti-inflammatory and analgesic effects in carrageenan-induced paw edema and anti analgesic activity effect in hot plate method. Results: Stem extracts of Pyrostegia venusta demonstrated the analgesic activity at dose of 50 mg/kg body weight. At the end of one-hour percent analgesia induced by petroleum ether extract is 26.34% and that by total methanolic extract is 40.69%. The total petroleum ether extract produced 43.10% inhibition of paw edema, methanolic extract showed 54.31% inhibition of paw edema. Both the extracts had comparable activity. Conclusions: Hence it revealed that Pyrostegia venusta stem extracts had potent...
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Posted by admin on Oct 31, 2018 in |
Objective: The aim of this pilot study was to assess the effect of processed Nigella Sativa (N. Sativa) on oligomenorrhea and amenorrhea in patients with Polycystic Ovarian Syndrome (PCOS). Materials and Methods: Ten women diagnosed with PCOS, having oligo-amenorrhea, participated in this study. The National Institutes of Health (NIH)’s criteria were used for the diagnosis. The patients, between 18 to 38 years old, were treated with 2 g/day of encapsulated processed N. Sativa for 16 weeks. This study compared menstruation habits before and after the medication. A series of metabolic and hormonal investigations was done at the beginning and the end of the study. Participants did not take any other medications. Monthly visits were done to assess the treatment. The effects of N. Sativa on the menstrual cycle was evaluated by four criteria: menstruation occurrence, menstruations intervals, menstruation severity and menstruation duration. The blood test results were analyzed using Stata version 13. Results: The average duration of menstruation and the ratio of cycle per month significantly increased during a...
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Posted by admin on Oct 31, 2018 in |
The treatment of cancer up to the saving of life of cancer patient is rare miracle. Cancer is deadly disorder (some cancers are diseases caused by viruses) if diagnosed in second or third stage leads to death, hence it is better to prevent it. The present study emphasizes on prevention of cancer rather than cure. Extraction of garlic was carried out by using maceration process. The liquid extract was concentrated under vacuum at 25 °C. Concentrated extract was exposed to UV light and packed in a suitable container for use in formulation development. Orodisperssible film formulation was developed by solvent casting method. In-vitro activity was evaluated on different cancer cell lines like human colon cancer cell line (COLO 205) and human prostate cancer cell line (PC-3). As compared to standard drug, Adriamycin (Doxorubicin) of allopathic origin, orodisperssible film formulation containing hydro-alcoholic garlic extract shows comparable tumor suppressive activity. Hence, orodisperssible film formulation containing stabilized garlic extract will find its place in nutraceutical market. To warrant further tumor suppressive activity...
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Posted by admin on Oct 31, 2018 in |
Bromelain is a protease present in pineapple fruit and stem. It is reported that commercially available pure bromelain has anticancer, anti-inflammatory and other therapeutic effects. But there are few reports available on the biological activity of bromelain along with peroxidase which is present in pineapple fruit extract. The aim of the present study was to investigate the effect of bromelain with peroxidase from pineapple fruit against lymphoma cell line in in-vitro. Pineapple was purchased from the local market from Tripura. Bromelain isolated along with peroxidase and enzyme activity tested with the substrate. In-vitro cytotoxicity was determined by MTT assay, and apoptosis was determined by fluorescence microscopy, DNA fragmentation assay and by western blot analysis. Fluorescence microscopy analysis and the MTT assay showed significant apoptosis of lymphoma cell after treated with bromelain along with peroxidase in a dose- and time-dependent manner and was more apoptotic than bromelain alone. This was further confirmed by DNA fragmentation assay. Furthermore, western blotting analysis confirmed the mitochondrial pathway is involved in apoptosis of lymphoma...
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