Posted by admin on Oct 31, 2018 in |
A simple, selective, rapid, precise and economical reverse phase high performance liquid chromatographic method has been developed for the simultaneous estimation of hydrocortisone and ketoconazole from pharmaceutical formulation. The method was carried out on a C18 (25 cm × 4.6 mm i.d., 5 μ) column with a mobile phase consisting of methanol: water (adjusted to pH 3.0 using triethylamine) in the ratio of 70:30 v/v. The retention time of hydricortusone and ketoconazole was 3.50 min and 6.00 min respectively with the flow rate of 1mL/ min. Eluents were detected at 221 nm. The linear regression analysis data for the linearity plot showed good linear relationship with correlation coefficient value for hydrocortisone and ketoconazole were R2 = 0.9995 and R2 = 0.9996 in the concentration range of 10 – 40µg. mL-1, 20 – 80 µg. mL-1 respectively. The relative standard deviation for intra-day precision was lower than 2.0 %. The method was validated according to the ICH guidelines. The method was also found to be robust. The developed method was...
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Posted by admin on Oct 31, 2018 in |
Condom is usually made up of thin rubber (latex) designed or is made of polyurethane to cover the penis sheath at closed one end that is inserted intravaginally during sexual intercourse for contraceptive purpose or as a means of preventive sexually transmitted diseases. Condoms should be safe, effective, user-controlled, non-prescription, easy to use and relatively cheap. The quality of the four different brand of condoms available in the local market was evaluated using standards given by international standards organization (ISO), such as; Bursting volume and pressure (inflation), width, length, thickness, electrical hole test, package integrity, lubricant quantity were done for each brand of condoms. The result showed that all sample of condoms have fulfilled the standards requirement set by ISO 407 and that all four brands of condom which is marketed in Addis Ababa Ethiopia are of safe, effective for the...
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Posted by admin on Oct 31, 2018 in |
The aim of this study was to develop microspheres based orodispersible tablets of roxithromycin using the solvent evaporation technique. The microspheres were formulated in to orodispersible tablets using direct compression technique by central composite design. The objective of the study was to analyze the effect of concentration of eudragit RS100 (X1) and concentration of polyvinyl alcohol (X2), on the entrapment efficiency of the drug. Tablets were evaluated for physical characteristics viz. weight variation, hardness, friability, disintegration time and in-vitro dissolution study. The hardness and weight variation of the tablet were found 4.7 ± 0.24 kg/cm2 and 346.74 ± 3.74 mg, respectively. Friability of tablets was found 0.54 ± 0.04 which is less than 1% or in the acceptable limit. Disintegration time and drug release were found 45 ± 0.51 seconds and 96.45 ± 2.24% in one hour, respectively. The drug release data were subjected to drug release kinetics...
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Posted by admin on Oct 31, 2018 in |
Bisphenol-A (BPA) is a chemical produced in large volumes worldwide and has large market diffusion in many consumer products. BPA is used mainly as a monomer in the production of polycarbonate plastics and as a precursor of epoxy resins. So the present study was undertaken to examine the toxic effect of BPA on heart function in pregnant rat models. BPA (50 mg/kg.b.wt/day and 500 mg/kg.b.wt/day) was administered for 8th to 15th day by oral-gavage. The rats were sacrificed by cervical dislocation after last treatment. Paraffin embedded heart tissue sections were stained with hematoxylin-eosin. Significant degenerative changes were observed in H&E stained heart tissue sections of test rats. The activities of antioxidant enzymes like- superoxide dismutase, catalase and glutathione peroxidase were seen to be decreased significantly in treated rats; while, the level of malondialdehyde, a biomarker of lipid peroxidation, was increased than treated rats. Up-regulation of proapoptotic protein has been reported in heart failure and myocardial infarction, to determine whether caspase-3 protein can affect cardiac function in treated pregnant rats....
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Posted by admin on Oct 31, 2018 in |
Here we have carried out the acute and repeated dose toxicity study on tubers of Pueraria tuberosa water extract (PTWE). For acute toxicity study, rats were orally administered a single dose of 2000 and 5000 mg/kg body weight of PTWE. For repeated dose toxicity study on rats in one set, the different doses were given daily up to 28 days to make general observation and mortality rate. In another set of experiment, group of different doses were sacrificed on 7, 14, 21 and 28 days to assess the biochemical and histological changes. The selected doses were 250, 500, 1000 and 2000 mg/kg b.w. To evaluate it, we have used OECD guidelines 425 and 407 respectively, in both acute and repeated oral toxicity. No adverse effect was observed in all the rats, up to 14 days after treatment with single doses of 2000 and 5000 mg/kg b.w. Slight increase in salivation, mild diarrhea and letharginess were observed on 7th day at dose of 500 and 1000mg/kg, but recovered on 14th...
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