Posted by admin on Oct 31, 2018 in |
Surgery alters the homeostatic balance and defence mechanisms in body eliciting certain responses called as stress response. In addition certain peri-operative factors may also influence the degree of stress response reflecting surgical recovery. The present study is an attempt to assess surgical stress response and to determine the influence of peri-operative factors on it. This study investigates the effects of surgery on early non specific immune and endocrine responses. 100 subjects undergoing minor and major elective surgeries were studied. Blood samples were collected before, immediately after and 72 h after the surgery. Total WBC count, differential neutrophil count, random blood sugar and pulse rate were assessed. Pain (Allina scale) and Depression Anxiety Stress scale (DAS) were used for evaluating the relation to stress response. In addition we used Estimation of Physiological Ability and Surgical Stress (E-PASS) scoring system for predicting the risk of post operative complications by quantifying patients reserve and degree of surgical stress. In minor surgeries, there was no significant drop in total counts after surgery, whereas...
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Posted by admin on Oct 31, 2018 in |
Background: Obesity is a confronting global issue with excess accumulation of body fat to an extent that decreases life expectancy. It is multifactorial in origin; genetic, environmental and pathophysiological. It leads to metabolic syndrome characterized by type 2 diabetes, cardiovascular diseases, fatty liver, hyperlipidemia etc. Objective: In this study, we have screened hydroalcoholic extracts of 7 plants for anti-obesity efficacy using inhibitory effect on lipid and carbohydrate metabolizing enzymes as indicators and further screening of these plants in 3T3 L1 cell line for inhibition of adipogenesis. Results: Among the 7 plants screened, Cyperus rotundus and Citrus limon showed highest inhibitory activity compared to other plants. These plants also showed antioxidant activity. Furthermore, these plants were investigated for their inhibition of adipogenesis and promotion of lipolysis in 3T3-L1cells. Cyperus rotundus and Citrus limon at 125 µg/mL showed inhibition of triglyceride accumulation and promoted lipolysis. Conclusion: Over all preliminary in-vitro screening study reveals Cyperus rotundus and Citrus limon showed potent anti obesity efficacy thus these plants may act as alternative herbal...
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Posted by admin on Oct 31, 2018 in |
Aim: To standardize Amritaprasha ghrita. Materials and methods: Physico‐chemical studies like refractive index, specific gravity, acid value, saponification value, iodine value, determination of unsaponifiable matter, peroxide, viscosity, rancidity test and HPTLC were carried out as per the WHO guidelines, Indian Pharmacopoeia and Ayurvedic Pharmacopoeia. Conclusion: Standardization tests done on Amritaprasha ghrita helped in authenticating and ensuring the quality of the...
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Posted by admin on Oct 31, 2018 in |
Proteases are one of the most important enzymes in many industrial applications, particularly as additives in laundry detergent industry. Hence, the present study investigates the isolation and purification of an extracellular protease from the Bacillus subtilis ASASBT isolated from termite soil sample. Among the five protease producers, one of the isolates was selected for further studies. It was related to Bacillus subtilis on the basis of 16S rRNA gene sequencing and biochemical properties. The maximum protease activity recorded at 48 h of incubation at 37°C was 154.13 U/ml. Based on the maximum activity of the enzyme, further optimization studies were done with Bacillus subtilis ASASBT. Its optima pH and temperature of enzyme production were pH 7.0 and 40 °C, respectively. Starch and gelatin were the best carbon and nitrogen sources for the production of protease. The isolated protease was purified in four steps involving ammonium sulphate precipitation, dialysis, DEAE-Cellulose and sephadex G-100 chromatography had a 4.01 fold increase in specific activity (86.51 U/mg) and 23.73 % increase in recovery...
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Posted by admin on Oct 31, 2018 in |
Background: The incidence of Diabetes Mellitus has increased dramatically in recent decades. Di Peptidyl Peptidase 4 inhibitors (DPP-4) have their role in glycemic control. An impaired ‘incretin effect’, occurs in patients with type 2 Diabetes Mellitus in response to glucose intake. Objectives: The main objective of the study is to compare therapeutic outcomes and adverse drug reactions among commonly prescribed anti diabetic drug combinations in adults with type 2 diabetes mellitus. Methodology: A retrospective and prospective study experimental study was carried out for a period of one year at care diabetes centre. Results: FBS and PLBS were found to be significantly lower in DPP group when compared with SU and TZ. Adverse events such as itching, abdominal pain, constipation and weight loss are more in DPP when compared with other groups. Data from this study indicated that DPP-4 inhibitors are superior to sulfonylureas (SU) and thiazolidinediones (TZ) when used in combination with metformin (B) in glycemic control. Conclusion: DPP inhibitors provide an effective therapeutic option for individuals with Type...
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