Posted by admin on Jul 31, 2018 in |
A stability indicating UPLC method was developed and validated for the determination of Teneligliptin hydrobromide and Metformin hydrochloride in bulk and tablet dosage form. The method was carried out using Endoversil column (2.1 × 50 mm, 1.8 mm) with mobile phase consisting of 0.1% v/v ortho-phosphoric acid: acetonitrile (80: 20) under isocratic mode with an injection volume 1 µl and at flow rate 0.3 ml/min and both the analytes were monitored at 221 nm. Stress conditions were performed by subjecting the individual analytes to the hydrolysis (acidic, basic), oxidation photolytic and thermal stress conditions. The retention times of Metformin hydrochloride and Teneligliptin hydrobromide were 0.443 and 0.694 min, respectively and showed a good linearity in the concentration range of 250 – 1250 µg/ml for Metformin hydrochloride and 10 – 50 µg/ml for Teneligliptin hydrobromide with a correlation coefficient of 0.9993 and 0.9990. The percentage recoveries of Metformin and Teneligliptin in the marketed dosage form found to be 99.86 and 99.96 respectively. The analytical performance of the proposed method was...
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Posted by admin on Jul 31, 2018 in |
In this study, an attempt was made to introduce a new mucoadhesive polymer – Borassus flabellifer fruit resin (BFR), especially for buccal drug delivery. The novel polymer, in combination with two other natural polymers (Pectin and Sodium alginate) and one synthetic polymer (PVA) were used to formulate buccal patches containing Metadoxine. Compatibility studies carried out with the help of FT-IR spectrometer indicated that there are no chemical interactions between the drug and the polymers used. The calibration graph of Metadoxine was obtained by a validated UV spectrophotometric method at a λmax of 324 nm. BFR was extracted from ripened palm fruit; stored and used for formulating 9 formulations in the ratios BFR : Pectin – 3:5, 4:4, 5:3 / BFR : SA – 4:2, 4:3, 4:4 and BFR : PVA – 3:5, 4:4, 5:3 respectively (the numbers in the ratios indicate the polymer concentration in percentage). Physicochemical properties such as thickness, weight variation, folding endurance, swelling index, surface pH, drug content and bioadhesion strength were evaluated appropriately and, the...
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Posted by admin on Jul 31, 2018 in |
Antiretroviral drugs are medications used for treatment of infection caused by retroviruses, primarily HIV. Different classes of antiretroviral drugs act at different stages of the HIV life cycle. No individual antiretroviral drug has been demonstrated to suppress an HIV infection for prolong treatment. Antiretroviral drugs must be taken in combinations in order to have a lasting effect. Combination of several (typically two or three or four) antiretroviral drugs were known to be defined as Highly Active Anti-Retroviral Therapy (HAART). These combinations create multiple obstacles to HIV replication to keep the number of offspring low and reduce the possibility of a superior mutation. If a mutation that conveys resistance to one of the drugs being taken arises, the other drugs continue to suppress reproduction of that mutation. In our Present study we aimed to develop a Stable Pharmaceutical dosage form with two different anti-retroviral drugs Lamivudine and Tenofovir Disoproxil Fumarate. Lamivudine and Tenofovir Disoproxil Fumarate were classified as High soluble and low permeable (BCS class 3) As per BCS classification...
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Posted by admin on Jul 31, 2018 in |
The main aim of the study was to scientifically validate the free radical scavenging activity of classically prepared Rasasindura (Red sulphide of mercury). With this intention Rasasindura (Red sulphide of mercury) was subjected to the screening of free radical scavenging activity in rat’s liver homogenate with four parameters like lipid peroxidation (LPO), super oxide dismutase (SOD), catalase (CAT) and reduced glutathiosone (GSH). In lipid peroxidation assay maximum decrease in concentration of MDA was seen in 1% of Rasasindura suspension in 1st, 2nd and 4th day of study. There was no significant difference when control and test sample compared with standard drug, this shows that the action of standard and test samples were same in concentration of SOD and GSH. 1% and 5% of Rasasindura has shown significant decomposition of H2O2 in 4th and 2nd day respectively when compared to standard group. There was no significant difference when control and test compared with standard drug. This shows that the action of standard and test drugs were same in decrease of...
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Posted by admin on Jul 31, 2018 in |
Olmesartan medoxomil (OLM) is an angiotensin II receptor blocker antihypertensive agent. It is a highly lipophilic (log p (octanol / water) 5.55), poorly water soluble drug with absolute bioavailability of 26%.The present work aimed at formulating a solid self Nano emulsifying drug delivery system (solid-SNEDDS) for Olmesartan medoxomil with the objective of improving the aqueous solubility, dissolution and oral delivery of the drug. The solubility of OLM was determined in various vehicles like oils, surfactants and co-surfactants. Pseudoternary phase diagrams were constructed for excipients to identify the efficient self-emulsifying region and proportions of various compatible excipients for the formulation. The liquid SNEDDS was a system that consists of Olmesartan, Labrafil m1944 CS, and tween 80, polyethylene glycol 400 as a drug, oil, surfactant and co-surfactant. The optimized liquid SNEDDS was transformed into a free flowing powder by using Neusilin US2 as adsorbent. Prepared SNEDDS formulations were tested for nanoemulsifying properties and the resultant nanoemulsions were evaluated for robustness to dilution, assessment of efficiency of self emulsication, emulsification time, turbidity...
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