Posted by admin on Jul 31, 2018 in |
The objective of the present study is to investigate the adverse effect of chloroquine (CQ) toxicity on reproductive tissues and its attenuation by curcumin. Chloroquine (CQ) has been used widely as an antimalarial drug due to its quick action on blood schizontocide of different malarial parasite species for prophylaxis and treatment of malaria. Thirty-six male mice were divided into six experimental groups: control, curcumin (80 mg/kg b.w.), CQ low dose (100 mg/kg b.w.), CQ mid dose (200 mg/kg b.w.), CQ high dose (300 mg/kg b.w.) and CQ high dose + curcumin treated group. An oral dose of artesunate and curcumin was administered for a period of 14 and 21 days to male Swiss albino mice. Results obtained indicated significant alterations in biochemical parameters as well as energy metabolism parameters in testicular tissue of animals after the treatment with chloroquine. Moreover, sperm count, sperm motility percent, sperm viability and sperm morphology of cauda epididymis of chloroquine treated mice revealed significant fluctuations as compared to the control group. Administration of curcumin...
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Posted by admin on Jul 31, 2018 in |
Proteomic study of Macrotyloma uniflorum is quite useful for exploration of its Calcium binding proteins (CBPs) and their antilithiasis character. CBPs play an important role in prevention of kidney stone formation. Considering this fact the study was design to identify CBPs in M. uniflorum. Total six samples were collected from different elevations of Uttarakhand. Total buffer soluble proteins of seeds were extracted according to the standard protocols. After quantification of protein with Bradford method 20 µg protein was loaded in 12% sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) gel according to Laemmli discontinuous system. CBPs were identified with the help of stains-all gel staining methods. In the proteomic study a protein (~44KDa) showed its distinct appearance when screened, the seeds from high elevation to low elevation. Three blue bands (CBPs) appear in all samples after stains-all staining. One high molecular weight protein (~43KDa) and two low molecular weight proteins (~25.1 and ~17KDa) respectively were observed. SDS-PAGE technique of seed proteins can be economically used to broad genetic variation and...
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Posted by admin on Jul 31, 2018 in |
Naturally occurring safe and broad spectrum UV blockers are required now- a- days to protect oneself from the onslaught of UV radiations that are known to cause many skin related ailments. The commercial inorganic sunblockers like Zinc oxide and titanium di oxide and organic sunblockers like Avobenzone and Benzophenone are known to be unstable and also their safety is doubted. Aim of the present communication was to study the secondary carotenoids like Zeaxanthin / Canthaxanthin / b– carotene extracted from thermotolerant genera Synechocystis pevalekii is screen for its role in skin protection against UV radiations. Solvents like Petroleum ether, Hexane, acetone, ethyl acetate, ethanol and methanol were used for extraction. Out of all these, freeze – thaw technique in 100% methanol proved to be the best solvent for extraction of carotenoids. The crude extract thus obtained was fractionated by silica gel open column chromatography and the resultant fractions were analyzed by HPLC. The HPLC analysis showed the presence of carotenoids like Zeaxanthin, Canthaxanthin and b- carotene. The next part...
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Posted by admin on Jul 31, 2018 in |
A new, simple, and rapid high-performance thin-layer chromatographic method was developed and validated for quantitative determination of Carbamazepine. Carbamazepine was chromatographed on silica gel 60 F254 TLC plate using Ethyl acetate: Methanol (9: 1 v/v) as mobile phase. Carbamazepine was quantified by densitometric analysis at 239 nm. The method was found to give compact spots for the drug. The linear regression analysis data for the calibration plots showed good linear relationship in the concentration range 100 – 600 ng/spot. The method was validated for precision, recovery, accuracy, and robustness as per the International Conference on Harmonization guidelines. The minimum detectable amount was found to be 15.096 ng/spot, whereas the limit of quantitation was found to be 45.696 ng/spot. Statistical analysis of the data showed that the method is precise, accurate, reproducible, and selective for the analysis of Carbamazepine. The method was successfully employed for the estimation of equilibrium solubility, quantification of Carbamazepine as a bulk drug, in commercially available...
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Posted by admin on Jul 31, 2018 in |
Excess fluoride causing fluorosis, can enter in to brain, disturb oxidative status and induce neural damage. Quercetin, antioxidant flavonoid stabilizes oxidative milieu of brain by quenching free radicals. The present study aimed to report ameliorative effect of quercetin on NaF induced oxidative stress, histological alterations in the brain and behavioural changes of rats. Rats were divided into four groups. The first group served as normal which received tap water. The second group was intoxicated with sodium fluoride (20 ppm/kg bw) through drinking water. The third group was given 20 ppm sodium fluoride in drinking water, along with treatment of quercetin (20 mg/kg bw) through oral gavage. The fourth group was treated with quercetin (20 mg/kg bw) through oral gavage. All animals were maintained for 60 days. Then, the antioxidant enzyme levels were measured, histopathological and behavioural studies were carried out. The results showed that the quercetin treatment significantly reversed the NaF induced increased lipid peroxidation (LPO) and decreased superoxide dismutase (SOD), catalase (CAT) and glutathione (GSH) levels in the...
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