Posted by admin on Jun 30, 2018 in |
Marine environment is a complex habitat defined by a wide fluctuation in conditions such as pH, temperature, nutrient availability, osmotic pressure and salinity. Because of these various extreme environmental conditions, it harbors varieties of biodiversity such as bacteria, algae, fungi, coral reefs and sea weeds, as compared to terrestrial environment. For adaptation in these conditions marine organisms developed new adaptative strategies like synthesis of secondary metabolites. Exopolysaccharides (EPSs) is one of the important secondary metabolites produced by marine organisms. The present study focuses on the isolation of marine bacteria from different sites of Bhavnagar coast Gujarat, India and screening for EPSs producing marine bacteria by various approaches viz., using fluorescent Calcofluor white dye, Congo-red and trypan-blue agar plate assay method. Quantification of EPSs was done by using phenol-sulphuric acid method for total carbohydrate content. In this work marine bacterial isolate Terribacillus saccharophilus strain PS – 47 gave copious amount of EPSs 1052 µg/ml. Isolate will be further selected for use in various biotechnological...
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Posted by admin on Jun 30, 2018 in |
Introduction: Herbs are the second most commonly used form of complementary and alternative medicine by public. The uses of medicinal herbs continue to increase worldwide. However, clinicians generally have limited knowledge and confidence about using of herbs in medicine. Therefore, this research aimed to assess the level of knowledge and attitude of primary care physicians toward use of medicinal herbs in order to protect the patients from the harmful effects of herbal medicines by increasing the physicians’ awareness regarding it. Method: A cross sectional study, includes 102 primary care physicians at Al-Wazarat health center in Riyadh. Results: 82.4% of the physicians believed that herbs are beneficial for disease management and only 42.2% of them believed that herbs should be used only when conventional medicine fails. 16.7% of the participants suggested that further education about herbs in medicine should be provided to physicians. However, the practice of herbal medicine was low and the level of knowledge was also low as all physicians scored less than 6 correct answers out of...
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Posted by admin on Jun 30, 2018 in |
The objective of this study was to evaluate the antidiabetic activity of methanolic extract of marine algae Sargassum polycystum (MSP) and Gracilaria edulis (MGE) in STZ induced diabetes in rats for Type I diabetic mellitus. The marine algae SP and GE were dried under shade and then powdered, and extracted with methanol. Preliminary phytochemical studies and acute toxicity studies were also carried out on methanolic extract of SP, and GE. After overnight fasting, for type I diabetes was induced in rats by I.P. injection of STZ dissolved in 0.1 M sodium citrate buffer at pH 4.5, at a dose of 65 mg/kg body weight. Group A served as normal control while group B was considered as diabetic control. Group C was standard receiving insulin 4U/kg and Group D diabetic animals were treated with MSP and MGE (250 mg/kg and 500 mg/kg) respectively. During the study, body weight and fasting blood glucose level were taken at 0 and 30th day. At the end of study, animals in all groups were...
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Posted by admin on Jun 30, 2018 in |
Diabetes mellitus is reaching potentially epidemic proportions in India. The level of morbidity and mortality due to diabetes and its potential complications are enormous and pose significant healthcare burdens on both families and society. In this study 400 blood serum samples were collected from various laboratories and diabetic centre in and around Tirupur, Tamil Nadu, India. Blood glucose level was estimated like Fasting Blood Glucose (FBG), Post Prandial Blood Glucose (PPBG), HbA1c and lipid content. Totally 215 (53.7%) samples were positive for diabetic. This study revealed that type 2 diabetic patients followed up in the diabetic center in Tirupur District, showed a high rate of chronic complications which often occurred in a mid age between 41 to 60 years and also hypertension and high cholesterol was not a major influence for diabetic complications. Numerous socio demographic and biological factors were significantly associated with the high rate of complications. Social and clinical factors significantly associated with high rate of complications were age between 41 to 60 years (74.87 %), male...
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Posted by admin on Jun 30, 2018 in |
The author has been proposed the precolumn derivatization method for the trace level quantification of cypionyl chloride in testosterone cypionate, it maybe potential impurity in the testosterone cypionate, it has to quantify in the testosterone drug Substance. The method development of cypionyl chloride has been started with gas chromatography with FID detector. The detection of cypionyl chloride was very poor and detection was less even for derivatization with 2-butanol in GC-FID detector. The GC-MS Electron impact ionization detector with derivatization by 2-butanol was developed and validated for trace level quantification of cypionyl chloride in Testosterone cypionate. The LOQ of this method was 1.7 ppm and the LOD was 0.6 ppm. The validation parameters were done against the ICH Q2 R1 guidelines. The precision, linearity, accuracy and solution stability was met the ICH Q2 R1...
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