Posted by admin on Aug 31, 2018 in |
The objectives of the present study were to use design of experiments (DOE) for formulation and optimization liposomal formulations and study the impact of process variables on quality attributes of the complex liposomal formulation system. Several factors may have contributed to the slow pace of commercialization of liposome drug products during the last decade: 1) the difficulties associated with identifying the formulation and process design critical quality attributes of these complex systems and 2) Higher manufacturing cost due to low preparation reproducibility and low entrapment of therapeutic active agents. Thus, a central composite design (CCD) was used for the optimization of the liposomal formulation. In the present study, the hydration volume, hydration time, sonication time and drug: lecithin ratio were chosen as input variables for the liposomal preparation and whereas; like particle size, peak shape, polydispersity Index, drug loading, entrapment efficiency and redispersion behavior were investigated as quality attributes. Additionally, contour plots and response surface plots were also utilized to understand the fundamental relationships between input variables and quality...
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Posted by admin on Aug 31, 2018 in |
Statins exhibit their pharmalogical effects by competitive inhibition trough binding with active sites of enzymes. Rosuvastatin, of all statines, has the most binding interactions with the enzyme, and being the most potent statin, it is presumed that the strength of enzyme binding directly influences its potency. Reduction of derivatives of mevalonic acids results with decreased risk of cardiovascular diseases and very significant pleotropic effect of statins. Rosuvastatin comes in the form of calcium salt. During the clinical trial it has been marked as superstatin with its proven activity in lowering cholesterol levels. Aim of this paper was to examine the pleotropic properties of rosuvastatin trough investigate if rosuvastatin shows antihyperalgesic / antiedemic effects by inflammatory pain model in rats trough effects of systemically applied rosuvastatin in inflammatory pain model in rats and effects of locally, peripherally applied rosuvastatin in inflammatory pain model in...
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Posted by admin on Aug 31, 2018 in |
The aim of this present investigation is to identify the most important risk factor for cancer by means of a mathematical model. The study begins with considering some types of cancer viz. breast, colorectum as well as lung and bronchus cancer as most of the cancer patients suffer from these types of cancers. Teaching-Learning-Based Optimization technique is applied to sorting out the most lethal cancer among all cancer consider in this study and it is found that lung and bronchus cancer is the most fatal. Further, we investigate the risk factor associated to lung and bronchus cancer by means of Literature, Expert and Local Hospital survey. All the risk factor has their own importance for death from cancer in medical aspects. Multi-Criteria Decision Making technique is applied to recognize the most significant risk factor among all the factors in statistical scenario. It is identified that smoking is the most concerning risk factor. The information related from the study may help to take necessary measure to control the death rate...
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Posted by admin on Aug 31, 2018 in |
Introduction: Green leafy vegetables are known for their anti-alzheimer potential. There are many leafy vegetables, however, that have not been examined scientifically for this activity. The present study was designed to evaluate antioxidant and antiacetylcholinesterase activities of two Brassica species namely B. napus and B. oleracea var. acephala, along with the comparison of their pharmacognostic characters and phytochemical profiles. Methods: A comparative evaluation of macroscopy, microscopy, physico-chemical parameters and qualitative phytochemical screening was carried out on leaves of the selected plants as per Indian Pharmacopoeia. Further, a parallel analysis of antioxidant and anti-acetylcholinesterase activities of both species was done using DPPH and Ellman assay, respectively, followed by standardisation of the extracts on the basis of total phenol and total flavonoid content. Results: The hydromethanolic extract of B. oleracea var. acephala showed higher radical scavenging activity than that of B. napus. Similar results were obtained in case of total phenol content (B. oleracea var. acephala: 15.18 ± 1.82% w/w, B. napus: 12.69 ± 2.26% w/w). The hydromethanolic extract of B....
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Posted by admin on Aug 31, 2018 in |
From time immemorial pharmacologists and researchers have always been in search for novel drug screening methods. One such unique technique that is gripping the world by its leash is “human tissue chip technology”. This study explores the creation of Multiple Sclerosis (MS) using human tissue chip and assessing the ability of Vasicine a quinazoline alkaloid derived from Adhatoda Justiciae plant in reversing the inflammatory pathology of multiple sclerosis. 10 ml of human peripheral blood was withdrawn using a sterile syringe from a healthy donor. The blood was centrifuged to obtain mesenchymal stem cells (MSC). Then the MSCs were transferred with Dulbecco’s Modified Eagle’s medium (DMEM). After 48 h incubation, the MSCs were transferred into four flasks with growth factors and were seeded into tissue chip for differentiation into neuronal cells, cardiomyocytes, hepatocytes, and skeletal muscle cells. The respective cells were dislodged from tissue chip for histopathological evaluation and differentiation confirmation. Lipopolysaccharide (LPS) was added to these cells, to mimic MS inflammation and was confined to histo-pathological assessment. 9 biomarkers...
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