Posted by admin on Jul 31, 2018 in |
Marine environment is a huge reservoir of various important resources for new bioactive molecule discovery from marine organisms. The aim of this investigation is to evaluate the anti-proliferative potential of crude proteins extracted from different organs of Donax variabilis (gills, adductor muscles, mantle and whole tissue) on human Lung (A549), oral (KB3-1), breast (MCF-7) and colon (HT-29) cancer cell lines. The crude proteins extracted from four different organs of marine clam were biochemically and qualitatively analysed using Native and SDS PAGE. These extracts were tested against normal (vero) cell line to determine the toxicity of crude proteins. Among the four organs, proteins from mantle potentially inhibited growth of lung, oral and breast cancer cell lines in a dose dependent manner and inhibition of growth in colon cancer cell line was time dependent when examined by MTT assay. Significant inhibition of cell viability on A549 cells observed at (IC50) 100 µg/ml, KB 3-1, MCF-7 cells at (IC50) 250 µg/ml concentration after 24 h of incubation and HT-29 at (IC50) 500...
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Posted by admin on Jul 31, 2018 in |
The main objective of the study is to evaluate orodispersible tablets of primaquine with different in-vivo parameters. Sharma P et al., (2015), in-vitro study was based on various evaluation parameters of orodispersible tablets of primaquine. From their study results, we have selected formula-6 of orodispersible tablets of primaquine for in-vivo study. Taste masking evaluation was performed on five male Swiss Albino rats (200-250 g). The in-vivo bioavailability study of drug were performed on six male white New Zealand rabbits (weighing 2-2.5 kg) and rabbits were randomly divided into two groups of equal size using parallel design. Next step of the study was HPLC analysis, in this study two conditions were followed by us. The conditions were- (a) Sample preparation: Protein precipitation method selected for extraction of drug from plasma because it was very simple and cost effective method for primaquine extraction. (b) Chromatographic conditions: HPLC-DAD-UV (Shimadzu Class-VP Japan) was used for evaluation of chromatographic conditions. With the help of UV and HPLC, marketed preparation (Primacip film coated tablet) and...
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Posted by admin on Jul 31, 2018 in |
Cordia dichotoma Linn. belonging the family Boraginaceae is locally called as Lasora in India. It is an important drug to cure diseases of the kidney, liver, spleen, heart and blood which attribute many medicinal properties in Ayurveda. The purpose of this study is to identify and characterize the bioactive principle from the bark of Cordia dichotoma Linn. Phytochemical screening methanolic extract of bark of Cordia dichotoma Linn. was done and showed the presence of phytosterol. Phytosterols were isolated from the methanol extract by admixing 100 g of extract with 200 ml of acetonitrile. The resulting white sterol crystals were analyzed by Thin Layer Chromatography and High Performance Liquid Chromatography (HPLC) for the purification. The sterol for structure elucidation was further studied by more sophisticated analytical techniques e.g. FTIR, 1H-NMR, 13C-NMR and LC-MS. After various spectral analysis and interpretation of same, the isolated compound was identified as...
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Posted by admin on Jul 31, 2018 in |
Dexamethasone drug induced the hyperglycemia in the Albino mice when intra-peritoneally administered at the dose of 10 mg/kg. Administration of dexamethasone raised the level of blood sugar in the mice. Administration of aqueous extract of C. citratus root and flower extract reduced the fasting and postprandial blood sugar levels, bringing them towards normal. We were observed anti-diabetic effect both root and flower extracts but the root had better anti-diabetic...
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Posted by admin on Jul 31, 2018 in |
The kinetic of conjugated reactions of S-oxidation and perhydrolysis of Piperacillin (Pip) with potassium hydrogen peroxo-monosulfate in alkaline medium is studied by the increase of forming product light absorbance. A scheme of peroxoacid oxidation and perhydrolysis conjugated reactions of Piperacillin by means of hydrogen peroxomonosulfate is proposed. The procedure of the quantitative analysis of Piperacillin in the combined with Tazobactam pharmaceutical preparation powder of Zopercin® (Piperacillin 4.0 g and Tazobactam 0.5 g) by spectrophotometric – kinetic method is elaborated using triple potassium Caro’s salt solution as a reagent. Peroxomonosulfate acid as triple potassium salt 2КНSO5∙КНSO4∙ K2SO4 (Oxone®) of “extra pure” qualification was used as oxidant. At pH 2-4 for 1 mole of Penicillin, 1 mole of KHSO5 is consumed; the quantitative interaction is achieved within a time of more than 1 min (observation time). The method on initial rates (tangent method) was used to collect kinetic data (usually at 280 nm) by following the appearance product of perhydrolysis reaction of Piperacillin. A solution of sodium hydroxide was thermostated in...
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