Posted by admin on Aug 31, 2018 in |
Purpose: Anacardium occidentale (Cashew) has been used by Folk medicines as a remedy for many diseases. In this study, we investigated the ameliorative activity of methanolic stem bark extract of Anacardium occidentale. Methods: Blood samples of the rats from each group were collected by tail bleeding for baseline biochemical test to know the state of the animals’ liver before the inducement of the injury. Thirty (30) Wistar rats were induced with CCl4 (1.5 ml/kg, b.wt, 20 % CCl4 / olive oil) for five days before treatment with the extract. After induction, elevated levels of the serum aminotransferases (ALT, AST), ALP and the decreased levels of ALB as compared to the enzyme markers levels before the induction of liver injury with CCl4 indicated hepatocellular injury. Results: Administration of the methanolic extract of Anacardium occidentale of different concentration (250, 500 and 1000 mg/kg b.wt oral) for five days reversed the impact of CCl4 toxicity significantly (P<0.05) on the serum markers ALT, AST and ALP of the damaged liver as compared to...
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Posted by admin on Aug 31, 2018 in |
The aim of this study was to investigate the antidiabetic and anti-inflammatory effects of probiotic Lactobacillus paracasei MCC3195 in high fructose fed type 2 diabetic rats. High fructose diet was used to induce type 2 diabetes in male albino wistar rats. L. paracasei MCC3195 was administered to rats at a single dose of 2×108 cfu/ml per rat for 8 week. The body weight, fasting blood glucose, oral glucose tolerance test, glycosylated hemoglobin, insulin, Liver glycogen level, lipid profile and oxidative stress parameters were analyzed. Interlukin-6 and Interlukin-10 were also estimated in serum. Histopathology analysis of pancreas and liver injury were done. At 8 week body weight, FBG, glucose tolerance, HbA1c, insulin, liver glycogen level, total cholesterol, triacylglycerol, LDL-C, VLDL-C, lipid peroxidation and IL-6 were significantly increased in diabetic rats, while administration of L. paracasei MCC3195 significantly decreased these factors. The level of HDL-C, reduced glutathione, superoxide dismutase, catalase and IL-10 were significantly decreased in diabetic rats, whereas administration of L. paracasei MCC3195 significantly increased these factors. Administration of L....
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Posted by admin on Aug 31, 2018 in |
Objective: Simple, rapid, sensitive, precise and reproducible specific UV spectrophotometric method for the determination of Aceclofenac (ACE) and Tramadol (TRM) in bulk drug and pharmaceutical dosage form were developed and validated. Methods: A simple double beam UV spectrophotometric method has been developed and validated with different parameters such as linearity, precision, repeatability, limit of detection (LOD), Limit of Quantification (LOQ), accuracy as per ICH guidelines. Results: UV-visible spectrophotometric method, measurement of absorption at maximum wavelength in 10 ml methanol and volume make with water solvent system as reference ACE and TRM were found to be at 203 nm and 241 nm respectively. The drug obeyed the Beer’s law and showed good correlation. Beer’s law was obeyed in concentration range 5 – 25 µg/ml for ACE and 2 – 10 µg/ml for TRM respectively with correlation coefficient was 0.999. The LOD and LOQ of ACE were found to be 4.7862 μg/ml and 14.50 μg/ml, TRM were found to be 2.0518 μg/ml and 6.2176 μg/ml, respectively. Percentage assay of ACE and...
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Posted by admin on Aug 31, 2018 in |
Green synthesis of nanoparticle using plant extracts is an efficient alternative for the conventional process of preparation of nanoparticles by chemical and physical methods which leads to non-eco-friendly by-products.The main aim of the project was to produce silver nanoparticles (AgNPs) using leaf extracts of Ayapana triplinervis (Eupatorium triplinerve) a medicinally important herb and to ascertain its antibacterial properties. AgNPs were synthesized by adding 1 ml leaf extract of A. triplinervis to 10ml of 3mM silver nitrate followed by incubation at 60οC. The AgNPs produced were further characterized by ultraviolet-visible (UV-Vis) spectroscopy, Fourier transform infrared (FT-IR) spectroscopy and scanning electron microscopy (SEM) analysis. UV-Vis spectroscopy of prepared silver colloidal solution showed absorption maximum between 460 – 490nm due to the excitation of surface plasmon resonance (SPR) of the AgNPs. FTIR spectra absorption peaks at 3431, 2065, 1635 and 530 cm−1 revealed the availability of functional groups which may be involved in the AgNPs synthesis. The SEM analysis showed that AgNPs were spherical or ellipsoidal in shape.The antibacterial activity against common...
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Posted by admin on Aug 31, 2018 in |
Ipomoea sepiaria Roxb., also known as Purple heart glory is a slender vine belonging to family Convolvulaceae. It is one among the ten sacred plants known collectively as “Dasapushpam” in Kerala. These plants are used for rejuvenating the body in the form of ‘karkidakakanji’ in the monsoon season in Kerala. Juice of the plant is used as deobstruent, diuretic, hypotensive, uterine tonic and antidote to arsenic poisoning. The present study aims at providing a detailed information of microscopic features of the leaf and stem. In cross sectional view the leaf exhibits short adaxial core, semicircular abaxial midrib and smooth lamina. The vascular bundle of the midrib is single, planoconvex and collateral. The vascular bundle includes wide, angular compact metaxylem elements and narrow circular protoxylem elements. Prominent glandular trichomes occur frequently on adaxial and abaxial epidermal layers. The glands of these trichomes are capitate type. The stem is circular, with short conical spiny ridges at certain places. It consists of intact epidermis, narrow cortex, elliptical hollow cylinder vascular tissues and...
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