Posted by admin on Jul 31, 2018 in |
There is unmet need in the treatment of stress induced depression on suggesting requirement of new therapeutic agent. A combination therapy of antidepressants sertraline (5 mg/kg), duloxetine (10 mg/kg) and agomelatine (8 mg/kg) are used in different combinations of doses by intra-peritoneal route. Swiss albino mice were used as an animal model for stress-induced depression; mice were divided into eight groups. Depression was induced through forced swim test, tail suspension test and sucrose preference test (chronic mild stress model). Efficacy of combination therapy was analyzed in specific regions of mice brain associated with depression (hippocampus, cerebral cortices and the whole brain) through brain monoamine estimation by HPLC (High- performance liquid chromatography) with fluorescence detector. Three drug combination [Agomelatine (2.67 mg/kg) plus duloxetine (3.33 mg/kg) plus sertraline (1.67 mg/kg)] showed antidepressant activity in in-vivo animal model testing parameters (decrease in immobility period, increase in swimming count and climbing count and increase sucrose consumption) than other treated group. In the same group, improvement in brain monoamine profile was observed illustrating antidepressant...
Read More
Posted by admin on Jul 31, 2018 in |
The crude malt obtained from the grains were hydrolyzed with cassava starch to obtain glucose syrup. 94.33% malt was produced from maize, rice had 84.46% malt, while sorghum had 84.97% malt. Glucose syrup yield was greatest using sorghum malt with percentage yield of 75.55%, 52.37% using rice malt, and while maize malt had 48.33 % syrup. Maize sample had highest malt yield but lowest glucose syrup yield showing that glucose syrup yield is in dependent on malt yield but on the quality of amylase produced during malting. Analysis on the glucose syrup obtained gave the following results: moisture content of 13.8%, 14.5%, 15.4%; ash content of 0.02%, 0.04%, 0.01%; carbohydrate 86.47%, 84.76%, 83.01%; total reducing sugar (invert sugar) 85.79%, 83.86%, 82.58%; total soluble (sugar brix) 100°Brix, 110°Brix, 105°Brix for rice, sorghum and maize samples respectively. Dextrose equivalent, viscosity, colour and taste of the end products were also analyzed. Microbial analysis was 2×101, 5×101, 3×101 cfu/mL for glucose syrup from rice, sorghum and maize samples respectively with no trace of...
Read More
Posted by admin on Jul 31, 2018 in |
Context: Acne vulgaris, a very common malady of adolescents, is well known and easily recognized. Acne is a “polymorphic” disorder which exhibits series of widespread and diverse lesions. Four key factors have been identified in the etiology of acne: increased sebum production, follicular hyperkeratinization, colonization of the pilosebaceous unit with Propioni bacterium acnes and the production of inflammation. Aims: To observe demographic and clinical profile of acne vulgaris patients. Settings and Design: The present study was carried out in the dermatology, venereology and leprosy department of a tertiary care teaching hospital. It was a prospective and observational study. Methods and Material: The study was carried out over a period from Jan 2013 to Feb 2014. The study protocol, performa and other documents approved by IEC. Statistical Analysis: Data was recorded and analysed in Microsoft Excel 2007 spread sheet. Results: Majority of the patients were from 12-20 years of age group. Most of the patients were female, unmarried, students, educated up to secondary level and had pityriasis infection as most...
Read More
Posted by admin on Jul 31, 2018 in |
The objective of current study was to develop self-nanoemulsifying drug delivery systems using long-chain triglycerides of resveratrol to enhance its bioavailability. Solubility studies performed in different lipids, surfactants and cosurfactants. Phase diagrams constructed to select areas of nanoemulsion. SNEDDS formulation optimized using 33 central composite design (CCD) considering lipid (X1), surfactant (X2) and co surfactant (X3) as critical variables and optimized formulation was located using overlay plot. The nanometer size and high values of zeta potential depicted non-coalescent nature of SNEDDS. Resulted SNEDDS formulation improved in vitro release followed by Hixson Crowell model with higher regression R2 value 0. 929. Thermodynamic stability studies ascertained stable formulation. Mean droplet size in selected nanocarrier was found to be 83.29 nm. The nanocarriers showed enhanced bioavailability in albino rabbits when compared to pure drug. The novel approach developed by selecting optimum blends of lipids, surfactants and cosurfactant using central composite...
Read More
Posted by admin on Jul 31, 2018 in |
The pretentious extract of Pseudomonas aeruginosa has shown promising anticancer activity. It contains a protein called Azurin. It has a molecular weight of 14 kDa. It is soluble in water. It is a type I copper-containing protein of cupredoxin family. In this work, Pseudomonas aeruginosa MTCC strain 647 was cultivated in modified Asparagine -proline broth. An attempt was done to isolate and purify azurin from Pseudomonas aeruginosa (MTCC 647) by using chromatography on sephadex G, CM cellulose and SDS PAGE electrophoresis. Recent studies have found that Pseudomonas aeruginosa can synthesize nano particles through either intracellular or extracellular mechanisms by bio synthetic means. Hence, the composite nano particles (Azurin-Ag NP) were synthesized by biosynthetic methods. P. aeruginosa MTCC strains 647 was cultivated in modified Asparagine -proline broth initially to produce shake flask cultures and then fed batch cultivation. From the bacterial extracted Azurin is isolated and purified to get a final concentration of 4.6 mg/g dry bacteria. As the nano particle mediated drug delivery effectively deliver the drug to the...
Read More
