Posted by admin on Jun 30, 2018 in |
We evaluated the total phenolic acid content and antioxidant activity of methanolic and aqueous extracts of Calycopteris floribunda roots. Antioxidant activity of the extracts was determined using Reducing Power Assay and Total Antioxidant Capacity (TAC) assays. Preliminary phytochemical analysis of the extracts revealed the presence of various phytochemicals. The total phenolic content of the extracts was higher in methanol than compared to aqueous extracts and subsequently higher antioxidant activity was also observed in case of methanol extracts. The results revealed that the antioxidant activity of the extracts was positively associated with the total phenolic acid...
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Posted by admin on Jun 30, 2018 in |
Phloroglucinol is a polyphenol and a component of phlorotannins belonging to the Laminariaceae family. Phloroglucinol has many positive effects on health. The present study was aimed to evaluate the antioxidant potential of phloroglucinol by comparing it with Standard antioxidant viz, ascorbic acid. The antioxidant potential of phloroglucinol was estimated using free radicals such as 1, 1-diphenyl-2-picrylhydrazyl (DPPH), superoxide anion (O2–), nitric oxide (NO–), hydroxyl radical (OH), hydrogen peroxide (H2O2) by standard methods. The study shows that phloroglucinol is more powerful against DPPH and hydrogen peroxide radicals, when compared to other radicals. The IC50 value of Phloroglucinol is 42 ± 1.00 µg/ml for DPPH, 53.66 ± 1.52 µg/ml for Nitric oxide, 102 ± 2.00 µg/ml for Superoxide, 180 ± 3.60 µg/ml for Hydroxyl and 52.3 ± 1.52 µg/ml for Hydrogen peroxide radicals. In-vitro methods suggest that phloroglucinol is effective against DPPH and hydrogen peroxide radicals. Thus our study showed that the phloroglucinol exhibited antioxidant activity against all the free radicals and could be considered as a source of natural...
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Posted by admin on Jun 30, 2018 in |
Present study includes the detailed pharmacognostic evaluation in terms of macroscopic and microscopic study along with physicochemical, phytochemical and fluorescence analysis of Capsicum annuum L., an annual spice and member of family Solanaceae, commonly known as red chili. The pharmacognostic studies provide comprehensive data not only in the identification but at the same time is an excellent tool for the evaluation of quality of spices. The Fourier Transform-Infrared spectrophotometer (FT-IR) analysis and elements detection by scanning electron microscope-energy dispersive x-ray spectroscopy (SEM-EDAX) was also performed and noted to be quite helpful in evaluation, authentication and judgement of quality of C. annuum. The scanning electron microscope-energy dispersive X-ray spectroscopy (SEM-EDAX) technique has been used in the present studies to detect the concentration of trace elements. Results of the present study indicated comprehensive information to check, evaluate and standardized commercial samples by the manufacturers and supplier with respect to purity, identity, safety, quality of red chili and other...
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Posted by admin on Jun 30, 2018 in |
Repaglinide is used in the treatment of type 2 Diabetes mellitus and has very short half life. The patient compliance can decrease when larger doses are administered two to three times a day. Sustained release formulation which maintains a plasma level of 8-10 h might be recommended for daily administration of repaglinide. The main objective of the present study was to develop sustained release formulation using hydrophilic release retardant natural and synthetic polymers i.e., Ocimum basilicum seed mucilage and HPMC K4M alone and in combinations respectively. Direct compression technique was used to prepare tablets which were evaluated for precompression and postcompression parameters. Nine formulations were prepared in which F1-F3 were prepared using Basil seed mucilage, F4-F6 by HPMC K4M and F7-F9 using both the polymers (Basil seed mucilage and HPMC) at 1:3, 1:6 and 1:9 to the drug and polymer ratios respectively. F8 was selected as the best formulation which sustained the drug release upto 98.61% in 10 h out of nine formulations (F1-F9). The selected formulation which was...
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Posted by admin on Jun 30, 2018 in |
A new, simple, sensitive, accurate and precise RP-HPLC method was developed for the estimation of quetiapine fumarate in human plasma. Full factorial design was used for the optimization of an extraction method. The main effect of volume of deproteinating agent, speed of centrifugation, time of centrifugation and temperature of centrifugation was found to be significant at P<0.0001 on all the responses. After deproteinization, the drug was analyzed on a C18 (150 × 4.6mm, 5 µm) column using UV detector. The mobile phase consisting acetonitrile and phosphate buffer (pH 3) in the ratio of 50:50 (v/v) at a flow rate of 1.0 ml/min. The standard calibration curve was constructed in the concentration range of 5 µg/ml to 30 µg/ml and linearity was found to be 0.999. Irbesartan was used as the internal standard. The retention time of quetiapine fumarate and the internal standard was found to be 5.42 and 2.89 min, respectively. No interference peak was perceived. The high performance liquid chromatography method was successfully demonstrated as rapid and sensitive...
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