Posted by admin on Jun 30, 2018 in |
Dental caries is a complex disease which is expressed as an interaction between various factors including the host, agent, substrate and time. The interplay between the dental biofilm and oral microorganisms is an important feature for development of a cariogenic dental plaque. Formation of the biofilm needs to be controlled through regular brushing in order to prevent the development of caries and periodontal diseases. Hence a study was conducted to evaluate and compare in-vivo the effect of two commercially available herbal and non-herbal (fluoridated) toothpastes on salivary bacterial count in 5 – 10 years age children with 3 – 6 decayed teeth. 40 children were randomly allocated into two groups I and II. Group I were provided with Fluoridated (Kidodent) toothpaste and Group II with Herbal (Babool) toothpaste. Participants were scored for plaque index and saliva sample was collected as the baseline score (day 0). After demonstration of brushing technique participants were asked to brush twice daily. The plaque index score and saliva sample was once again collected at...
Read More
Posted by admin on Jun 30, 2018 in |
A simple, accurate and reproducible HPTLC method has been developed for the simultaneous quantification of diosgenin, oleic acid, harmine and kaempferol from the methanolic extract of fruits of plant Tribulus terrestris L. and its marketed formulation. The CAMAG HPTLC systems with winCATS software used for the analysis. Double development with mobile phase Toluene: Ethyl acetate: Formic acid: Glacial acetic acid (2:1:1:0.75 v/v/v/v) on TLC plate silica gel 60 F 254. Detection and quantification of harmine at 366 nm, kaempferol at 270 nm, diosgenin at 201 nm and oleic acid at 580 nm after derivatization with anisaldehyde sulphuric acid reagent. The quantity of harmine was found to be 0.014% and 0.006%, kaempferol was 0.018% and 0.006%, oleic acid was 0.259% and 0.108% and diosgenin was 0.086% and 0.022% in plant and formulation respectively. The developed method was then validated in terms of specificity, linearity, LOD, LOQ, precision and recovery. The validated method was successfully applied for quantification of four components in a formulation containing Tribulus terrestris L....
Read More
Posted by admin on Jun 30, 2018 in |
Plant mucilages have found wide application in food, pharmaceutical and cosmetic industries. Hence, it would be worthwhile to characterise the physical and chemical attributes of mucilages from commonly available plants for their beneficial utilization. Hibiscus rosa-sinensis Linn. (Family Malvaceae), widely grown as an ornamental throughout the tropics and subtropicsis a good source of mucilage. The present study focuses on the extraction of the mucilage from the leaves of Hibiscus rosa-sinensis, its subsequent quantification and physico-chemical characterisation. A protocol was presently standardized for the yield optimization of mucilage from Hibiscus through detection of an appropriate maceration temperature and precipitation solvent. Further analyses revealed ideal pH value, swelling index, flow properties and particle size of Hibiscus mucilage. Moreover, chemical, FTIR spectral and electrokinetic data of Hibiscus mucilage revealed that it can be considered as a suitable blending ingredient in composites with potential industrial...
Read More
Posted by admin on Jun 5, 2018 in |
Coumarin segment have been reported broad spectrum of different biological activities with the oxazine derivatives. In view that we have synthesized newer coumarin clubbed 4-(4-fluorophenyl)-6-substituted phenyl-2H-1,3-oxazin-2-amine and screened for their biological studies. 4-(4-fluorophenyl)-6-substituted phenyl-2H-1,3-oxazin-2-amine IIa-j condensed with 4-Methyl-6-nitro-2-oxo-2H-chromen-7-yl chloro acetate 5 to afford 4-Methyl-6-nitro-2-oxo-2H-chromen-7-yl-2-(4-(4-sustitutedphenyl) oxazine – 2- yl – amino) acetates 2a-j. The newer compounds were characterized by IR, NMR and mass spectral studies and were screened for their antimicrobial, antitubercular and antioxidant activities. Compound 2a (-C6H5) 62.5 µg/ml, 2d (2-OH) 100 µg/ml against S. aureus, 2a (-C6H5) 50 µg/ml, 2f (4-F) and 2j (3-OPh) 100 µg/ml against S. pyogenes as compared to chloramphenicol and the other compound 2c (4-Cl) showed excellent potency 25 µg/ml against E. coli and 12.5 µg/ml against P. aeruginosa while compound 2e (4-OH) exhibited comparable activity of 62.5 µg/ml and 100 µg/ml against E. coli and P. areuginosa respectively. Furthermore 2i (3-Br) also showed 100 µg/ml moderate potency against E. coli compared to the standard drugs chloramphenicol and ciprofloxacin. The study of antifungal activity...
Read More
Posted by admin on Jun 1, 2018 in |
By considering the current regulatory requirement for an analytical method development, a reversed phase high performance liquid chromatographic method for routine analysis of cobicistat has been developed using analytical Quality by design approach. The optimized method was achieved using unisol C-18 (3 μm, 4.6 ×150 mm) column with mobile phase consisting of mixture of water and methanol (70: 30 v/v) with a flow rate of 1ml/min at 240 nm. The optimized method was then validated according to the ICH guidelines. The developed method was found linear over the concentration range of 10-80 μg/ml and the detection and quantitation limit was found to be 0.39μg/ml and 1.2μg/ml. There are no interfering peaks under performed degradation conditions. Therefore, a sensitive, robust, accurate and stability indicating method was developed with high degree of practical...
Read More
