Posted by admin on Feb 29, 2024 in |
The quantitative measurement of Axitinib and Avelumab has been created using a simple, quick, precise, sensitive, and reproducible reverse-phasehigh-performance liquid chromatography (RP-HPLC) method. It is more difficult to analyse varying amounts of pharmaceutical active medicinal ingredients in dose forms without any interferences. Therefore, the objective of the current work is to estimate Axitinib and Avelumab simultaneously by adopting an Analytical Quality by Design (AQbD) arotatable central composite-based technique using RP-HPLC-based method development and validation. Axitinib and Avelumab were separated by chromatography using a Hyperclone 5µ BDS C18 130A (150 x 4.6 mm, 5μ) column and a mobile phase made up of Acetonitrile: 0.1% TEA pH-2.5/OPA in a ratio of 45:55 v/v. The flow rate was 1.2 ml/min, and a Photodiode Array Detector operating at room temperature was used to detect absorption at 219 nm. ICH criteria have been used to validate the offered techniques’ linearity, accuracy, precision, and other attributes. The degradation study’s findings showed that the medications deteriorated in high-stress situations. The chemical and pharmaceutical sectors might easily...
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Posted by admin on Feb 29, 2024 in |
Medication Adherence is defined as “the extent to which a person’s behavior in taking medication, lifestyle management, diet, corresponding with an agreed recommendation from a healthcare provider”. Hypertension also called high blood pressure is defined as persistency with an elevation in systolic blood pressure ≥140 mmHg and /or diastolic pressure ≥ 90 mmHg. A cross-sectional study was carried out in order to measure medication adherence, Morisky Green Levine-Medication Adherence Questionnaire developed by Donald Morisky was used to assess medication adherence assessment, which consists of 4 questions (items) whose wording questions are formulated to avoid answering ‘yes’ or ‘no’ and three levels of medication adherence: high, medium, and low were measured based on the scores 0, 1-2,3-4 respectively. The study carried out an evaluation of medication adherence among hypertensive patients. Among 245 respondents, 5.7% were found to be highly adherent, 37.6% were found to be medium adherent and 56.7% were found to be low adherent. A significant association between a family history of hypertension, diabetes, and smoking behavior was observed....
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Posted by admin on Feb 29, 2024 in |
C. splendens is a medicinal plant used for various ailments within the West-African Community. This study sought to investigate the pharmacognostic characteristics, mycobactericidal, and mutagenic activities of the hydro-ethanolic leaves extract C. splendens. The organoleptic, microscopic, macroscopic and physicochemical characteristics were investigated according to WHO, 2011 quality control methods for herbal materials. The mycobactericidal and mutagenic activities were investigated by the agar well diffusion assay and the Ames test (Muta-ChromoPlateTM) respectively. Present in C. splendens are tannins, flavonoids, glycosides, saponins, steroids and alkaloids which are comparable to published literature. The leaves of had anomocytic stomata, and starch granules, while calcium oxalate crystals were visibly absent. The 50% ethanol extract of C. splendens was not active against M. smegmatis at the highest concentration of 100 mg/mL. C. splendens was also non-mutagenic. Though the extract exhibited no mycobactericidal activity at the highest tested concentration, the documented pharmacognostic characteristics can be used for the purposes of authentication. Also, the non-mutagenic potential indicates a lack of toxicity in that...
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Posted by admin on Feb 29, 2024 in |
Aspartame is the most common artificial sweetener which is known to induce oxidative stress. Our goal was to determine whether Boeravinone B and Chebulinic acid protect HepG2 cells against Asp-induced oxidative stress in HepG2 on a multifarious level. In this investigation we have addressed ameliorative role of BB and CA in due course of Asp exposure by evaluating biochemical and molecular parameters. We found that Aspartame induced oxidative stress in HepG2 cells through elevation of ROS and RNS which was further attenuated by BB and CA. Associated levels of SOD, CAT, GPx, Protein carbonyls, Lipid peroxides and Caspase 3/7 in Asp treated cells were found to be risen but BB and CA supplementation restored these back to normal. It was revealed from fluorescence microscopy as well as flow cytometric analysis that BB and CA reversed the proportion of apoptotic, necrotic as well as autophagic cells which was found to be triggered during Asp exposure. mRNA expression of marker genes associated with Apoptosis, Necrosis and Autophagy was observed to be...
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Posted by admin on Feb 29, 2024 in |
Changes in drug prescribing-pattern in different groups of population and age-related pathophysiological changes may result in altered response of drugs and need proper monitoring to avoid adverse consequences. Polypharmacy is very common in elderly which further increases risk for unwanted effects. Moreover, physicians should be more familiar with the common problems and diseases occurring amongst elderly population and the drugs safe for this age group. Present study has been done to know the pattern of diseases occurring amongst elderly and commonly prescribed drugs for this population. Study-design was cross-sectional and it included private medical practitioners at Lucknow. Patients above 60 years of age attending these physicians were interviewed personally using a questionnaire. Thus, this study describes pattern of medication consumption by elderly patients among community dwelling in urban and rural areas of Lucknow. Out of 112 subjects interviewed, 51.5% elderly were prescribed three or more medications. Overall consumption varied from 1 to 10 different medications per day. Out of all medications prescribed; antihypertensives ranked first followed by analgesics, sedative-hypnotics,...
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