Posted by admin on Jul 31, 2018 in |
Ulcerative colitis is an idiopathic chronic inflammatory disease of the colon for which a lot of treatment modalities are present, however, significant side effects are associated with them and there is a need for a search for other treatment options. This study was aimed to investigate the therapeutic role of Doxycycline in experimentally induced colitis in rats. Animals were categorized into 5 groups; the control group undergoes no induction of UC, colitis group in which UC was induced and animals receive no treatment, the Doxycycline group that received Doxycycline and Sulfasalazine group that received Sulfasalazine. Each group was composed of 10 animals. After the completion of the one-month period of experiment, animals were sacrificed and the following measures were done: weight of colon, determination of the area of mucosal damage by mm2, histological scoring after hematoxylin and eosin stain together with MAC score and immunohistochemistry of IL-6, TNF-alpha, MPO, MDA, CD62, and ICAM1. The results revealed that Doxycycline was able to reduce the area of mucosal damage, colon weight,...
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Posted by admin on Jul 31, 2018 in |
The main aim of the study was to formulate the buccal films of most satisfactory formulation by in-vitro evaluation. The present investigation was done to formulate Hydralazine hydrochloride with an objective to improve therapeutic efficacy, Patient compliance. Hydralazine hydrochloride buccal films were developed by solvent casting method. Formulation H2, P4, S8, was best fitted to the in-vitro release studies and zero order release was...
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Posted by admin on Jul 31, 2018 in |
Rumex obtusifolius belong to the family Polygonaceae, it is a common wayside weed and it has a good medicinal value. In the present study, the methanolic extract (sample named as R2) of roots of R. obtusifolius was used to examine the in-vitro and in-vivo anti-inflammatory activity using human red blood cell membrane stabilization method to measure the potential of the extract on human red blood cell membrane. Diclofenac sodium was used as a standard drug. The percentage of membrane stabilization for methanolic extract of roots of R. obtusifolius was done at concentration 200 µg/ml. It was concluded that methanolic extract of R. obtusifolius showed a good anti-inflammatory effect against the standard drug. This study states that examination of the anti-inflammatory effect of methanolic extract of R. obtusifolius for the improvement of medicinal...
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Posted by admin on Jul 31, 2018 in |
Marine environment is a huge reservoir of various important resources for new bioactive molecule discovery from marine organisms. The aim of this investigation is to evaluate the anti-proliferative potential of crude proteins extracted from different organs of Donax variabilis (gills, adductor muscles, mantle and whole tissue) on human Lung (A549), oral (KB3-1), breast (MCF-7) and colon (HT-29) cancer cell lines. The crude proteins extracted from four different organs of marine clam were biochemically and qualitatively analysed using Native and SDS PAGE. These extracts were tested against normal (vero) cell line to determine the toxicity of crude proteins. Among the four organs, proteins from mantle potentially inhibited growth of lung, oral and breast cancer cell lines in a dose dependent manner and inhibition of growth in colon cancer cell line was time dependent when examined by MTT assay. Significant inhibition of cell viability on A549 cells observed at (IC50) 100 µg/ml, KB 3-1, MCF-7 cells at (IC50) 250 µg/ml concentration after 24 h of incubation and HT-29 at (IC50) 500...
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Posted by admin on Jul 31, 2018 in |
The main objective of the study is to evaluate orodispersible tablets of primaquine with different in-vivo parameters. Sharma P et al., (2015), in-vitro study was based on various evaluation parameters of orodispersible tablets of primaquine. From their study results, we have selected formula-6 of orodispersible tablets of primaquine for in-vivo study. Taste masking evaluation was performed on five male Swiss Albino rats (200-250 g). The in-vivo bioavailability study of drug were performed on six male white New Zealand rabbits (weighing 2-2.5 kg) and rabbits were randomly divided into two groups of equal size using parallel design. Next step of the study was HPLC analysis, in this study two conditions were followed by us. The conditions were- (a) Sample preparation: Protein precipitation method selected for extraction of drug from plasma because it was very simple and cost effective method for primaquine extraction. (b) Chromatographic conditions: HPLC-DAD-UV (Shimadzu Class-VP Japan) was used for evaluation of chromatographic conditions. With the help of UV and HPLC, marketed preparation (Primacip film coated tablet) and...
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