Posted by admin on Jul 31, 2018 in |
The study was conducted to evaluate the synergistic effect of EDTA with antibacterial (Ceftriaxone + Sulbactum) as a combinational therapy against antioxidant enzyme activity, lipid peroxidation level, some hematological and biochemical parameters in Klebsiella pneumoniae induced nosocomial pneumonia followed by histopathology of lungs tissue in experimental exposed Sprague Dayle (S.D) rats. Total 24 rats were selected and divided into 4 groups of 06 rats each for in-vivo and in-vitro EDTA / antibacterial therapy. Group I Control normal saline treated group; group II was infected via K. pneumoniae bacterial strain. Group III and IV were infected plus treated group with antibacterial and EDTA / Antibacterial drugs. The results indicated that a significant (p<0.005) decrease in enzymes activities (Superoxide dismutase, catalase and ascorbic acid) along with increased lipid peroxidation level as well as cytokine parameters (tumour necrosis factor-α, interlukin-6) and biochemical parameters in plasma of infected group as compared to control group. These activities were increased along with decreased in lipid peroxidation level and biochemical parameters in both treated group as...
Read More
Posted by admin on Jul 31, 2018 in |
The adverse effects of oxidative stress have been discussed widely in the present day. It is the main cause of protein denaturation, cardiovascular diseases, ageing, etc. Thus, the daily requirement of anti-oxidants has to be fulfilled through dietary sources. Inflammation is yet again another form of stress on the body. It is the major cause of multiple chronic disorders. It can lead to the formation of many other disorders such as arthritis, asthma, macular degeneration, etc. Arthritis is one such inflammatory disorder which is associated with swelling of joints, joint pain, etc. This research was based on the anti-oxidant, anti-inflammatory and anti-arthritic properties of tea extracts such as Camellia sinensis, Hibiscus rosa sinensis, Rosa, Zingiber officinale, Matricaria chamomilla. Ex-vivo tests for evaluating antioxidant property of samples using liver tissue by LPO assay was performed. In addition, tests such as inhibition of protein denaturation and RBC membrane stabilization were performed to determine anti-inflammatory effect of the sample. Collagen denaturation assay using chicken collagen to check anti-arthritic properties of samples were...
Read More
Posted by admin on Jul 31, 2018 in |
The objective of the present study is to investigate the adverse effect of chloroquine (CQ) toxicity on reproductive tissues and its attenuation by curcumin. Chloroquine (CQ) has been used widely as an antimalarial drug due to its quick action on blood schizontocide of different malarial parasite species for prophylaxis and treatment of malaria. Thirty-six male mice were divided into six experimental groups: control, curcumin (80 mg/kg b.w.), CQ low dose (100 mg/kg b.w.), CQ mid dose (200 mg/kg b.w.), CQ high dose (300 mg/kg b.w.) and CQ high dose + curcumin treated group. An oral dose of artesunate and curcumin was administered for a period of 14 and 21 days to male Swiss albino mice. Results obtained indicated significant alterations in biochemical parameters as well as energy metabolism parameters in testicular tissue of animals after the treatment with chloroquine. Moreover, sperm count, sperm motility percent, sperm viability and sperm morphology of cauda epididymis of chloroquine treated mice revealed significant fluctuations as compared to the control group. Administration of curcumin...
Read More
Posted by admin on Jul 31, 2018 in |
Proteomic study of Macrotyloma uniflorum is quite useful for exploration of its Calcium binding proteins (CBPs) and their antilithiasis character. CBPs play an important role in prevention of kidney stone formation. Considering this fact the study was design to identify CBPs in M. uniflorum. Total six samples were collected from different elevations of Uttarakhand. Total buffer soluble proteins of seeds were extracted according to the standard protocols. After quantification of protein with Bradford method 20 µg protein was loaded in 12% sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) gel according to Laemmli discontinuous system. CBPs were identified with the help of stains-all gel staining methods. In the proteomic study a protein (~44KDa) showed its distinct appearance when screened, the seeds from high elevation to low elevation. Three blue bands (CBPs) appear in all samples after stains-all staining. One high molecular weight protein (~43KDa) and two low molecular weight proteins (~25.1 and ~17KDa) respectively were observed. SDS-PAGE technique of seed proteins can be economically used to broad genetic variation and...
Read More
Posted by admin on Jul 31, 2018 in |
Naturally occurring safe and broad spectrum UV blockers are required now- a- days to protect oneself from the onslaught of UV radiations that are known to cause many skin related ailments. The commercial inorganic sunblockers like Zinc oxide and titanium di oxide and organic sunblockers like Avobenzone and Benzophenone are known to be unstable and also their safety is doubted. Aim of the present communication was to study the secondary carotenoids like Zeaxanthin / Canthaxanthin / b– carotene extracted from thermotolerant genera Synechocystis pevalekii is screen for its role in skin protection against UV radiations. Solvents like Petroleum ether, Hexane, acetone, ethyl acetate, ethanol and methanol were used for extraction. Out of all these, freeze – thaw technique in 100% methanol proved to be the best solvent for extraction of carotenoids. The crude extract thus obtained was fractionated by silica gel open column chromatography and the resultant fractions were analyzed by HPLC. The HPLC analysis showed the presence of carotenoids like Zeaxanthin, Canthaxanthin and b- carotene. The next part...
Read More
