Posted by admin on Aug 31, 2018 in |
We have developed Ag2Ga nanoneedles and achieved individual nanoneedles with diameter ranging from 200 to 250 nm with sharp tip and micro scale length required for a drug carrier to deliver drugs to site of application. Nanoneedles are designed to be inserted in human tissue and therefore they have to be compatible with the local environment of both in terms of toxicity and intended function. The variation of toxicity results for various nanostructures were reported in literatures. Hence, it is important to assess the information about toxicity of novel needle shaped nanostructures. Acute toxicity study was performed to examine the acute dermal toxicity of Ag2Ga nanoneedle mediated silver gel formulation on the rat’s skin, according to the OECD Guidelines Test no.402. Additionally, acute dermal irritation study was also performed to examine the irritancy potential following single, 1 h and 4 h, semi occluded application of Ag2Ga nanoneedle preparation on the rat’s skin according to OECD Guidelines Test no. 404. Acute toxicity study observations showed no skin toxicity or mortality...
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Posted by admin on Aug 31, 2018 in |
Lymphatic Filariasis is one of the most abandoned tropical diseases caused by the parasite, Brugia malayi. The existing conventional drugs act generally on the larval stages of the parasite. The enzyme asparaginyl tRNA synthetase is an excellent molecular target as it plays a crucial role in protein synthesis. Evidences based on the literature presented clues to discover the flavonoids as potential anti-filarial leads, which led to the scope for this computational study. The computational parameters such as docking score, binding energy, intermolecular hydrogen bond interaction and the identical amino acids confirm that flavonoids could serve as prospective anti-filarial agents. The outcomes prove that they can be further explored in in-vitro and in-vivo studies to authenticate their claim as potential anti-filarial...
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Posted by admin on Aug 31, 2018 in |
About 70 middle aged (20 – 45 years) patients, including 40 males and 30 females, operated at Base Military Hospital, Meerut Cantonment Board, Meerut (India) developed typical symptoms of pus formation at surgical sites and opening of sutures after 30 – 45 days. To investigate the causative pathogen and source of infection, various samples were collected from wounds and various sites of Operation Theatre. The samples were processed on culture media including nutrient agar, McConkey agar and Lowenstein-Jensen’s medium (LJ medium). To identify the pathogens, Gram’s and acid fast staining of the smears of samples and cultures obtained on different media, were carried out. Acid fast bacilli were observed after 3 – 5 days of incubation on LJ medium. The wound samples showed the prevalence of Mycobacterium chelonae and acid fast...
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Posted by admin on Aug 31, 2018 in |
A simple, sensitive, and high-performance liquid chromatography ultraviolet detection (HPLC-UV) method was developed and validated for the quantification of palbociclib in human plasma. Plasma samples were processed by solid phase extraction using an Oasis hydrophilic-lipophilic balance extraction cartridge (1 mL, 30 mg). Commercial imatinib was used as an internal standard. Palbociclib and imatinib (IS) in human plasma were analyzed using a mobile phase of acetonitrile and 0.1% Triethylamine TEA (pH 3.3; adjusted with 50% Ortho-phosphoric acid) (70:30, v/v), on a Agilent Zorbax C18 column (150 × 4.6 mm i.d., 5 µm) using isocratic elution at a flow rate of 1.0 mL/min with ultraviolet detection at 266 nm. The method was validated over a concentration range of 0.1-3.0 µg/mL (r2 ≥ 0.998) with coefficient of variation for intraday precision of 3.92%, 3.56% and 2.28% for 0.5, 1.5, and 2.5 µg/mL respectively. The lower limit of detection was 50 ng/mL. The extraction recovery rates for palbociclib ranged from 66.69% to 80.97%. The intra- and inter-day precision was below 5.0%, and the...
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Posted by admin on Aug 31, 2018 in |
Atherosclerosis is a condition where the arteries become narrowed and hardened due to an excessive build up of plaque around the artery wall. The disease disrupts the flow of blood around the body, posing serious cardiovascular complications. Liver X alpha receptor (LXRα) is highly expressed in liver, function as cholesterol sensors with important roles in regulating cholesterol homeostasis. The leaves of Corchorus trilocularis possess anti-atherosclerotic property along with its anti hyperglycemic activity. The Genetic Optimization for Ligand Docking (GOLD) software resulted in identifying the best compound that interacts with the receptor. The present study was carried out to evaluate the anti-atherosclerotic activity of the ethanolic leaf extract of Corchorus trilocularis against LXRα by...
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