Posted by admin on Jul 31, 2018 in |
A new, simple, and rapid high-performance thin-layer chromatographic method was developed and validated for quantitative determination of Carbamazepine. Carbamazepine was chromatographed on silica gel 60 F254 TLC plate using Ethyl acetate: Methanol (9: 1 v/v) as mobile phase. Carbamazepine was quantified by densitometric analysis at 239 nm. The method was found to give compact spots for the drug. The linear regression analysis data for the calibration plots showed good linear relationship in the concentration range 100 – 600 ng/spot. The method was validated for precision, recovery, accuracy, and robustness as per the International Conference on Harmonization guidelines. The minimum detectable amount was found to be 15.096 ng/spot, whereas the limit of quantitation was found to be 45.696 ng/spot. Statistical analysis of the data showed that the method is precise, accurate, reproducible, and selective for the analysis of Carbamazepine. The method was successfully employed for the estimation of equilibrium solubility, quantification of Carbamazepine as a bulk drug, in commercially available...
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Posted by admin on Jul 31, 2018 in |
Excess fluoride causing fluorosis, can enter in to brain, disturb oxidative status and induce neural damage. Quercetin, antioxidant flavonoid stabilizes oxidative milieu of brain by quenching free radicals. The present study aimed to report ameliorative effect of quercetin on NaF induced oxidative stress, histological alterations in the brain and behavioural changes of rats. Rats were divided into four groups. The first group served as normal which received tap water. The second group was intoxicated with sodium fluoride (20 ppm/kg bw) through drinking water. The third group was given 20 ppm sodium fluoride in drinking water, along with treatment of quercetin (20 mg/kg bw) through oral gavage. The fourth group was treated with quercetin (20 mg/kg bw) through oral gavage. All animals were maintained for 60 days. Then, the antioxidant enzyme levels were measured, histopathological and behavioural studies were carried out. The results showed that the quercetin treatment significantly reversed the NaF induced increased lipid peroxidation (LPO) and decreased superoxide dismutase (SOD), catalase (CAT) and glutathione (GSH) levels in the...
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Posted by admin on Jul 31, 2018 in |
The aim of study was to evaluate cardioprotective activity of fulvic acid in doxorubicin induced cardiac toxicity. In current investigation, female/male Wistar rats were divided into five groups, normal, control, fulvic acid 100 mg/kg, fulvic acid 200 mg/kg, and fulvic acid 300 mg/kg. Doxorubicin 15 mg/kg was administered in six divided doses to all except normal group for 2 weeks. Fulvic acid was administered to respective groups once daily for total 21 days, on the last day of study, the animals were anesthetized to record ECG and BP (by cannulating carotid artery), blood was collected from carotid artery and SGOT, LDH and CK-MB were estimated, animals were sacrificed to isolate heart and preparation of tissue homogenate. The antioxidant status is analyzed by measuring MDA content, SOD, CAT, GSH activity. The tissue sample is also preserved for histological studies. Doxorubicin cause cardiac damage which was manifested by alteration in serum cardiac markers, antioxidant markers, ECG and hemodynamic and histological changes. Which were assuaged due to treatment with fulvic acid 300...
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Posted by admin on Jul 31, 2018 in |
Cancer has emerged as a major health problem globally as a consequence to the increased longevity of the population, changing environment and life style. Chemoprevention is a new and promising strategy for reducing cancer burden. The present study was conducted to evaluate the potential of Quinine sulphate to prevent chemically induced skin cancer in mice. Cancer was induced on 2-stage skin carcinogenesis model by single topical application of 7,12 dimethylbenz [a] anthracene (DMBA), as initiator, and two weeks later it was promoted by croton oil treatment thrice a week on the dorsal surface of mice for 16 weeks. Mice were divided into following groups: I Normal mice without any treatment, II Topical Acetone treated, III DMBA + Croton oil (Positive Control), Group IV Topical quinine sulfate treated (higher dose), Group V Topical quinine sulfate treated (lower dose). The differences in the values of the results of experimental groups were statistically analyzed which were significant in comparison to the control group. The animals of the quinine sulphate treated group had...
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Posted by admin on Jul 31, 2018 in |
There are so many promising plant based chemical constituents are present which act as alternative therapy for the control of diabetes. But due to lack of its proper quality control parameters they are not widely used. The US FDA patent is approved for fixed dosage combination of Mangiferin (MF) and Berberin HCl (BER) as antidiabetic herbal formulation. Absorbance ratio method uses the ratio of absorbances at two selected wavelengths, one which is an iso-absorptive point and other being the λmax of one of the two components. From the overlay spectra of two phytomarkers, it is evident that MF and BER show an iso-absorptive point at 317 nm. The second wavelength used is 257 nm, which is the λmax of MF. The drug response with respect to absorbance was linear over the concentration range 5 – 30 μg/ml for MF and 10 – 60 μg/ml for BER. The percentage recovery of MF and BER as found to be 100.00% and 100.07% respectively. The method can be successfully employed for the...
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