Posted by admin on May 1, 2018 in |
Background: Extensive use of analgesics like non-steroidal anti-inflammatory drugs, opioid is associated with undesirable side effects often limiting their use. Hence the search for indigenous safer analgesic agent is ongoing. It was considered worthwhile to evaluate analgesic activity of methanolic fraction of ethanolic extract of Crocus sativus (CSEEMF) stigmas. Objective: This study was conducted to evaluate analgesic activity of methanolic fraction of ethanolic extract of Crocus sativus in rats and mice. Material and method: After obtaining permission from animal ethics committee, the animals were divided into 5 groups of 6 animals each control, standard – Aspirin (75 or 150 mg/kg) or Pentazocine (5 mg/kg), CSEEMF. Antinoceceptive activity of stigmas of CSEEMF was investigated at doses 50 mg/kg, 100 mg/kg and 200 mg/kg through tail-flick method, tail clip method and formalin test in swiss albino mice and writhing test by using acetic-acid in wistar albino Rats. Results: One-way analysis of variance (ANOVA) followed by Dunnett’s test was applied in the study. Both p<0.05, p<0.001 were considered to be significant. In...
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Posted by admin on Apr 30, 2018 in |
Oxidative stress is one of the factor that initiating development of cataract and describes the events leading to lens opacification, generally cataract is a clouding of the lens of the eye that will affect the vision either partially or completely. Proteins that are present in the lens begin to break down and the lens becomes cloudy. This will be avoided by taking external food supplements that are rich in antioxidants. Morinda citrifolia fruit (Noni) juice is rich in antioxidants. This food supplement with lot of nutraceuticals support good health and diminish the effects of aging. To investigate the involvement of Noni in protection against oxidative stress, chick lens epithelial cells were subjected with hydrogen peroxide (100 µM H2O2) over a time course, with and without pre-treatment of Noni fruit extract and commercial Noni. Na+K+ATPase enzyme estimation was performed to determine the role of Noni juice and Noni extract. Our study revealed that Noni juice and extract preserved physiological functions of chick and human lens epithelial cells when subjected to...
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Posted by admin on Apr 30, 2018 in |
Andrographis paniculata (Burm. F) Nees, commonly known as the “king of bitters,” is an herbaceous plant belonging to the Acanthaceae and is found throughout tropical and subtropical Asia, Southeast Asia, and India. Andrographolide, a bitter diterpenoid, is the major active principle present in the plant. Authors have already worked on the phytochemical investigation, analgesic and antipyretic activities of ethanolic extract of Andrographis paniculata. Present study aimed to pharmacokinetic study of an ethanolic extract of Andrographis paniculata tablet in rat. Solid dispersion tablets of Andrographis paniculata extract equivalent to 50 mg/Kg was prepared and given to rats and various pharmacokinetic parameters such as maximum concentration of andrographolide in plasma (Cmax), elimination rate constant (K), Elimination half-life (t1/2) Area under the plasma andrographolide concentration- time curve up to measurable concentration (AUC(0- t)), The area under the curve zero to infinity (AUC0-∞), mean residence time (MRT) and relative bioavailability (Frel) were determined and also compared with pure andrographolide. Results showed that there significant increase in various pharmacokinetic parameters of solid dispersion tablet...
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Posted by admin on Apr 30, 2018 in |
ABSTRACT: To find new antiviral agents from natural sources, methanol and aqueous extracts of leaves and fruits of Ribes uva –crispa and Ribes multiflorum naturally grown in Turkey were investigated as in vitro to reveal their antiviral activities against Herpes simplex virus type 1. All experiments were performed in 96-well plates and results were measured with XTT-based colorimetric assay. Results demonstrated that all extracts have no cytotoxic effect on vero cells at 10000 µg/mL, while they also exhibited anti-HSV-1 activity with different percentages of protection (varying between 2.65% – 50.40%) in 10000 µg/mL which was at the highest concentration in vero cells. 50% effective concentrations (EC50) of the extracts which were determined having percentage of protection against HSV-1 at concentrations lower than 10000 µg/mL were calculated using GraphPad Prism Version 5.03 statistics program with non-linear regression analysis. These extracts were determined to have EC50 values ranging between 9710 – 70600 µg/mL and selectivity index (SI) are ranging between 0.14 – 1.03. On the basis of these results we believe...
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Posted by admin on Apr 30, 2018 in |
Objective: Pergularia daemia and Terminalia catappa L.. Leaf extracted with hydroethanol and the extracts were separated by silica gel column chromatography. The selected column fractions are used in GC-MS analysis, from the GC-MS results eight phytochemical compounds are selected based on their biological activities. In the present work we predict the interaction of the selected targeted proteins (CYP2E1, UDP-GT, GST and Nrf2 binding site in keap1 protein,IL-6) with bioactive compounds of the extracts (HAEPD and HAETC) by molecular docking studies. The number of bioactive compounds present in HAEPD and HAETC, and there phytochemicals are responsible for various biological as well as hepatoprotective and anti hepatotoxic properties of the plant...
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