Posted by admin on Jun 30, 2018 in |
This study was carried out to investigate the acute oral toxicity LD50 of an anti-neoplastic and a chemotherapeutic agent hydroxyurea in male albino mice by using up α Down method. The result revealed that LD50 of hydroxyurea was (7868.5 mg/kg. b.w). Orally after 24 h of exposure. That’s mean it is slightly toxic agent. To study the effect of lethal doses of hydroxyurea on the some of blood parameters, two survival animals were taken from this study which was received (7000 mg/kg. b.w and 7500 mg/kg. b.w ) orally and compared with normal values of blood parameters in male albino mice. The results revealed there is an increase in Hb comparing with the normal values and the increase was dose dependent manner, while the results showed decrease of RBCs, WBCs, PCV and lymphocytes in animals received hydroxyurea comparing with the normal values and the decrease was dose dependent...
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Posted by admin on Jun 30, 2018 in |
In this research work an attempt was made to formulate and evaluate in-situ gel for the intranasal delivery of montelukast sodium for the effective treatment of asthma. Thermally gelling in-situ gel formulations for nasal administration were prepared by using polymers such as poloxamer 407 and HPMC K4M in varying concentrations by using cold method. The formulated in-situ gels were evaluated for clarity, pH, gelation temperature, gel strength, viscosity, drug content and in-vitro drug diffusion studies. The drug-polymer compatibility was determined by using FTIR studies. On the basis of clarity studies formulations F3, F4, F5 and F6 were selected for further evaluation testing because all of these formulations were found clear in appearance as compared to formulations F1 and F2 which showed hazy appearance and thus rejected. Further the drug release data obtained from the selected formulations were fitted to various kinetic models such as zero order, first order and Higuchi matrix kinetic models. Finally, based on the results obtained from all the evaluation parameters formulations F5 and F6 were...
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Posted by admin on Jun 30, 2018 in |
Endophyte fungi reside in the tissues beneath the epidermal cell layers and live within a plant for at least part of its life without causing apparent diseases. Eight morphologically distinct endophytic fungi were selected from the medicinal plants Elaeocarpus sphaericus coded as ELSPF 1 to 4 and Myristica fragrans coded as MYFRF 1 to 4. The highest colonization frequencies (CF %) were found to be 12.69%, and 8.33% for ELSPF 1 and MYFRF 2. Internal transcribed spacer- polymerase chain was performed for molecular identification and ~600 bp amplified products were obtained. The sequences were analysed by BLAST and the phylogenetic tree was constructed with neighbour joining method. The fungi were cultured on Potato Dextrose broth for production metabolites. The metabolites were extracted with ethyl acetate and constituent phytochemicals were analyzed. The crude extracts were tested for antimicrobial activity by agar well diffusion method. The test organisms used for the antimicrobial activity studies were E. coli, Staphylococcus aureus and Klebliella pneumonia. The ethyl acetate extracts of all endophytic fungi showed...
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Posted by admin on Jun 30, 2018 in |
The positive results of screening medicinal plants for antimicrobial activity forms primary platform for phytochemical and pharmacological studies in future. The work was undertaken to evaluate the antimicrobial activity of four medicinal plants viz., Sesbania grandiflora, Epiphyllum oxypetalum, Nyctanthes arbor-tristis, and Vetiveria zizanioides. Leaves were extracted using water and ethanol as solvents. The antimicrobial activity was assessed against five bacterial and fungal pathogens like Bacillus subtilis, Staphylococcus aureus, Escherichia coli, Salmonella typhi, Enterobacter aerogenes and Aspergillus flavus, Aspergillus niger, Cladosporium cladosporioides, Fusarium monoliforme, Penicillium sp. Aqueous extracts of S. grandiflora was found to be most effective in inhibiting B.subtilis (23 ± 0.66) while ethanolic extracts showed N. arbor-tristis inhibiting S.typhi (20 ± 0.35). Further aqueous extracts of S. grandiflora showed considerable effect of inhibition against Penicillum sp. (22.63 ± 0.60) whereas ethanol extracts of S. grandiflora was most efficient against Penicillium sp. (24 ± 0.33). All the other plants extracts were also effectual to remarkable extent. The present investigation has thrown a light on the effect of ethanolic leaf...
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Posted by admin on Jun 30, 2018 in |
Kinetics and stoichiometry S-oxidation reaction of sodium piperacillin by means of potassium hydrogen peroxomonosulfate in aqueous solutions using iodometric titration were studied. Zopercin® – powder in vials for piperacillin injection solution in the combined form with tazobactam (piperacillin 4.0 g and tazobactam 0.5 g) was used for analysis. A new iodometric method for quantitative determination of sodium piperacillin in Zopercin® preparation using potassium hydrogen peroxomonosulfate (KHSO5) as analytical reagent was proposed. Peroxomonosulfate acid as triple potassium salt 2KНSO5∙KНSO4∙K2SO4 (Oxone®) of “extra pure” qualification was used as oxidant. At pH 2-4 for 1 mole of penicillin, 1 mole of KHSO5 is consumed; the quantitative interaction is achieved within a time of more than 1 minute (observation time). The results were obtained by the recommended procedure for seven replicate titrations of mixtures containing the three species at various concentrations. RSD = 1.15 %, δ = (+0.2) %. It can be seen that piperacillin can be determined successively with good accuracy and reproducibility. The new procedure was developed and ability of quantitative...
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