Posted by admin on Jun 30, 2018 in |
Pyrazoline derivatives, being used as potential medicinal agents, 3-Acetyl-2H-chromen-2one (I) was prepared by Knoevenagel condensation of salicyladehyde with ethylacetoacetate in presence of piperidine. A series of 3-[(2E)-3-substituted-prop-2-enoyl]-2H-chro men-2-one derivatives (II a-h) were prepared by Claisen-Schmidt condensation of 3-acetyl coumarin with aromatic aldehydes. Treatment of 3-substituted cinnamoylcoumarin with hydrazine hydrate in the presence of ethanol gave [5-substitutedphenyl]-4, 5-dihydro-1H-pyrazol-3-yl]-2H-chromen-2-one (III a-h). Title compound were synthesized by conventional as well as by microwave assisted method. The structures of the newly synthesized compounds were confirmed by IR and 1H-NMR spectroscopy. All the synthesized compounds were tested for their antibacterial and antifungal activities using cup-plate-agar-diffusion method. The antibacterial activity screening revels that the compound III b has comparable activity and compound III c shows moderate activity as that of standard ampicillin against gram positive and gram negative bacteria. All synthesized compounds were found to be inactive as antifungal against Candida...
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Posted by admin on Jun 30, 2018 in |
The present study aimed to develop and validate stability indicating method for the simultaneous determination of Elbasvir and Grazoprevir in its pharmaceutical dosage form using RP-HPLC. Chromatographic separation was done with Discovery C18 (250 mm × 4.6 mm, 5 µ) column using 0.1% ortho-phosphoric acid (OPA) and acetonitrile in the ratio 50:50% v/v as mobile phase on isocratic mode. The column oven temperature was maintained at 30 ºC with a flow rate 1.0 ml/min and components were detected at a wavelength of 315 nm. The retention times for Elbasvir and Grazoprevir was found to be 2.24 min and 3.21 min respectively. The developed method was validated according to ICH guidelines. A good linearity response was observed in the concentration range of 12.5 µg/ml – 75 µg/ml for Elbasvir and 25 µg/ml – 150 µg/ml for Grazoprevir with correlation coefficient of 0.999 for both the drugs. The method was found to be accurate, precise, specific, rugged and robust. The drugs were subjected to stress conditions for testing their stability and...
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Posted by admin on Jun 30, 2018 in |
ABSTRACT: Background: Postoperative pain control is an important part of effective postoperative care. However, 30 – 80% of postoperative patients complain about moderate to severe post-surgical pain as a result of the surgical procedure or a pre-existing disease. Aim: To assess the safety and efficacy of different modalities of postoperative pain management in post surgical wards of a tertiary care hospital. Method: It’s a prospective study was conducted in 213 patients of either gender, aged between 18 – 65 years. Patients were followed up from surgery till discharge or till the 4th postoperative day, the drugs given for pain relief both as single drug and combination drugs and adverse effects were monitored. The severity of pain was assessed using a numeric pain rating scale after 1 h of surgery, then followed by 2 h, 4 h, 6 h, 12 h, 24 h, 48 h and 96 h. Patient satisfaction with the analgesic therapy given was recorded at the time of discharge or after 4th postoperative day using 5 point...
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Posted by admin on Jun 30, 2018 in |
Background: It becomes clear that living processes were based on the action of enzymes. Cell immobilization serves as a useful tool for the immobilization of intracellular enzymes. The purpose of study is to improve economically important processes by attaching the yeast cells to matrices. Methods: In this analytical study by using mixed models of matrices like (sodium alginate alone, sodium alginate and gelatin, sodium alginate and agar, sodium alginate and glutaraldehyde) to determine the effective combination for immobilizing cells without any damage. Results: It was noted that the glucose content in both cells increased which indicates the production of carbon dioxide and alcohol and then the values started falling down this is because the osmotic concentration of the sugar gets so great that the yeast is unable to get enough water for growth. Conclusion: Glucose utilization was analyzed in free and immobilized cells on four different matrices for verifying their performance and the improvement of immobilized yeast cells over free yeast cells. Use of mixed matrices enhanced the biological...
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Posted by admin on Jun 30, 2018 in |
Lannea coromandelica is one of the important herbal plants well distributed in India. It is commonly called as Odiar, Gumphini in South India. It was extensively used by the tribes all over India to treat various diseases such as body pain, tooth ache, stomach ache, ulcers, sexual impotency. The aim of the present study was to evaluate the GC-MS analysis, antibacterial and anticancer activities of methanol bark extract of L. coromandelica. The methanol extract was subjected to GC-MS analysis to find out active volatile and semi-volatile compounds. The antibacterial activity was carried out by well diffusion method by using nutrient broth agar medium. Microorganisms such as Escherichia coli, Staphylococscus aureus, and Bascilus subtilis were used to evaluate antibacterial activity. The maximum zone of inhibition 21 mm was measured for Bascilus subtilis at 500 µg/mL concentration followed by Staphylococscus aureus was 19 mm and Escherichia coli was 18 mm. The anticancer activity was carried out by MTT assay method for MCF7 cell line. The maximum cell death was 74.20 ±...
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