Posted by admin on Apr 21, 2018 in |
This is a simple, economic, sensitive stability indicating RP-HPLC method for the simultaneous estimation of metformin and teneligliptin in bulk and pharmaceutical formulation. The method was carried out on octa-decyl C18 column (5 μm, 25 cm × 4.6 mm, i.d) using methanol: water in the ratio of 70:30 and pH of the mobile phase up to 3 was adjusted with OPA at a flow rate of 1.0 ml/min. The wavelength for metformin and teneligliptin at 235 nm was found to be appropriate. The linearity range was obtained in the concentration range of 25 – 150 μg/ml for MET, while 5 – 30 μg/ml for TNG respectively. The retention time of metformin and teneligliptin was found to be 2.45 and 6.68 min, respectively. The regression equation for MET and TNG were found to be as y = 8.288 x + 0.026 and y = 27.26 x + 38.28 with correlation coefficient (R2) 0.999 and 0.999, respectively. The developed method was found to be robust, accurate and sensitive which can be...
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Posted by admin on Apr 9, 2018 in |
Pharmacological activities have been reported for the seed extracts of Lepidium sativum L.; however, the plant has not been widely studied for its effects on the thyroid gland. Objective: The present study was designed to investigate to possible potential pharmacological activity of ethanolic extract of Lepidium sativum L. seeds on regulation of thyroid hormone concentrations in male rats. Methodology: Each experiment was performed on Male Wistar rats, which were randomly divided into 4 groups. Group I received normal saline for 21 days and served as normal control. Group II received extract seeds of L. sativum at a dose of 200 mg/kg i.p. for 21 days. Groups III was treated with a 25 μg/100 g intraperitoneal (i.p.) T3 injection for 21 days and; Group IV was treated iopanoic acid (IOP; 100 mg/kg) for 21 days. Results: The levels of the rats’ serum Triiodothyronin (T3), Thyroxin (T4) and Thyroid-stimulating hormone (TSH) were analyzed. Thyroid gland specimens were processed for microscopic examination. Group II treated with extract seeds of L. sativum showed significant decline T3...
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Posted by admin on Apr 5, 2018 in |
Oral films are a novel approach for the development of orally disintegrating dosage forms. They are thin, elegant in appearance and can be made into various sizes and shapes like rectangular or circular. The strips may be flexible or brittle, opaque or transparent. Oral films are made up of various hydrophilic and hydrophobic polymers to provide rapid disintegration on the tongue without the need for water. The aim of the present study is to develop fast dissolving films of tramadol Hydrochloride by enhancing drug dissolution in the oral cavity. The development of the films decreases the analgesic effect in less time and increases the patient’s compliance. Tramadol HCl films were prepared using Hydroxy propyl methyl cellulose-E5, Hydroxy propyl methyl cellulose-E6, Hydroxy propyl methyl cellulose-E15 and sodium alginate as polymers. Therefore the prepared films were evaluated for various parameters like physical appearance and surface texture of the film, thickness of the film, folding endurance, moisture uptake, uniformity of weight and drug content, swelling index, in vitro disintegration studies, and in...
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Posted by admin on Apr 3, 2018 in |
Chemometric designs were applied to develop a simple UV-visible spectroscopic method for the simultaneous estimation of Hydrochlorthiazide (HCT), Amlodipine (AMLO) and Telmisartan (TEL) in bulk and solid dosage form. The simultaneous spectroscopic method was developed for the three drugs and the data generated from the spectra were determined by using Chemometric methods such as trilinear regression analysis, Cramer’s matrix method, Method of least squares, Multivariate calibration methods such as partial least square regression (PLS) and Principle component regression (PCR). The wavelengths selected for all the above methods were 270 nm (wavelength of maximum absorption; λmax of HCT), 342 nm (wavelength of maximum absorption; λmax of AMLO) and 292 nm (wavelength of maximum absorption; λmax of TEL). Results: The methods shows good linearity for TEL from 4 – 20 μg/ml, for HCT from 2-10 μg/ml and AMLO from 2 – 10 μg/ml with regression coefficient values of 0.970, 0.996 and 0.980 respectively. The RSD value for intraday and inter-day precision was found to be less than 2%. The percentage recovery...
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Posted by admin on Mar 31, 2018 in |
The present study aimed to green synthesis the nanoparticles using bioactive compounds from unique seaweeds and testing its application for anti-microbial, anti-diabetic, ovicidal, larvicidal and cytotoxic activity. Seaweeds are marine non- flowering plants have much of the secondary metabolites and had not been explored widely. There are very limited amount of literature are there for seaweeds. Steochospermum marginatumis a species of brown algae in the family Phaeophyta, they are found on rocky shores. The green synthesis of silver nanoparticles (AgNPs) was synthesized from the seaweed Steochospermum marginatum. The AgNPs was formed at 60°C at 5000 rpm when the solution of silver nitrate was added to aqueous extract of Steochospermum marginatum. The UV-Visible Spectroscopy, Fourier transform-infrared spectroscopy (FT-IR), Scanning electron microscope (SEM) were used for characterization of AgNPs. The green synthesized silver nanoparticles were tested for various applications such as Anti-microbial, ovicidal, larvicidal and anti-diabetic and cytotoxicity using VERO cell...
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