Posted by admin on Feb 29, 2024 in |
A simple, precise and sensitive High Performance Liquid Chromatographic method has been developed for the quantitative estimation of Lafutidinein bulk and tablet dosage form. The method was performed on Silicagel 60 F254 aluminum plate. A Camag HPTLC system comprising of Camag Linomat IV semiautomatic sample applicator, Camag TLC Scanner 3, Camag twin-trough chamber (10×10 cm) and Camag CATS 4 software, As mobile phase Ethyl acetate: Toluene: Methanol: Ammonia (6:2.5:1.5:0.1%v/v) was utilized to develop the Chromatographic method. Lafutidinegave separation at Rf value of 0.45±0.03 and scanned at 273 nm. The calibration curve response was observed between 400-1400ng/spot. The linear regression data showed good linear relationship with R2 value of 0.99895. The method was validated in terms of repeatability, precision, accuracy, and specificity according to ICH guidelines. The LOD and LOQ were found to be 19ng/Spot and 59 ng/spot respectively. The developed method was successfully used for assay of Lafutidine in tablet...
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Posted by admin on Feb 29, 2024 in |
In view of rising concern of problem associated with synthetic drugs against inflammation, current research has been focused towards safer, effective and natural compounds. Hence, we aimed at assessing the anti-inflammatory effect of four plants from Solanum species (S. seaforthianum, S. diphyllum, S. sisymbriifollium and S. anguivi) through albumin denaturation assay, trypsin inhibition and RBC membrane stabilization assays. The data showed that the methanol extracts of these plants had concentration dependent protection. S. anguivi extract showed maximum inhibition of albumin denaturation (26.15 ± 0.08%), whereas S. sisymbriifollium extract displayed maximum (16.51 ± 0.002%) trypsin inhibition. With respect to heat induced RBC lysis, S. anguivi extract showed highest inhibition (26.16 ± 0.08%), whereas S. seaforthianum extract displayed maximum inhibition (36.49 ± 0.002%) of RBC lysis induced by hypotonicity. From the study, it can be inferred that anti-inflammatory activity in the tested Solanum species extract varied considerably and its activity can be attributed to its richness in phytochemical...
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Posted by admin on Feb 29, 2024 in |
The Cu-Atropine complex may have potential applications in medicinal chemistry, as atropine is used in the treatment of certain medical conditions such as bradycardia and as a pre-anesthetic to decrease salivation. Additionally, the complexation of atropine with copper could potentially enhance its therapeutic effects or alter its pharmacokinetic properties. In the present study, the drug has been modified by complex formation with absolute methanol and concentrated sulphuric acid following standard procedures to increase its potency. IR spectra of Cu- atropine complex has taken. The analytical data indicates that a complex form of the drug, Cu-Atropine, is formed in a 1:1 ratio. To study the interaction between drugs and metal complexes, differential pulse polarography (DPP) has been used. These techniques are simple and cost-effective, making them suitable for qualitative and quantitative determination of active drug compounds. In a pharmacological study, modified atropine showed more potency compared to its parent...
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Posted by admin on Feb 29, 2024 in |
The Indian medicinal plant Boswellia serrata, sometimes known as frankincense in the West, has been used in traditional medicine to heal burns and wounds. The objective of this study was to investigate the capability of B. serrata (Boswegex®) to enhance wound healing by carrying out an in-vitro scratch experiment on human dermal fibroblast cells (HDF). Povidone-Iodine 5% cream was used as a positive control to determine the efficacy of Boswegex®‘s wound healing activity. The cytotoxicity, along with proliferation and migration activities, were evaluated against the human dermal fibroblast cell line HDF using the MTT [3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium] assay. Interestingly, Boswegex® was found to stimulate proliferation and migration of HDF cells at given concentrations and enhance the closure rate of the wound area within 24 hours after treatment. Boswegex® was evaluated for its cytotoxicity at different concentrations ranging from 1000 µg/ml to 0.975 µg/ml, which resulted in 7.78±0.06 µg/ml and hence the concentrations of 2µg/ml and 1µg/ml. In the scratch assay, 349.62µM and 234.8 µM migrations of cells were exhibited...
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Posted by admin on Feb 29, 2024 in |
The objective of the research was to compare the efficacy and safety of Olanzapine versus Risperidone as an add-on to Lithium carbonate in patients with bipolar affective disorder. Methods: Prospective, randomized, comparative study conducted in psychiatric Department from Nov 2018 to May 2020. 80 BPAD patients who fulfilled eligibility criteria were randomized (1:1) into two groups of 40 each receiving Olanzapine with Lithium (Group A) or Risperidone with Lithium (Group B) for 12 weeks. Efficacy was measured by reduction in Young Mania Rating Scale (YMRS) score and Hamilton Depression Rating Scale (HAM-D) score at 6th and 12th week. Safety was assessed by monitoring treatment emergent adverse effect. Data analysed using repeated measure ANOVA, Mixed ANOVA, chi-square and descriptive statistics. Results: Olanzapine and Risperidone treated BPAD patients showed significant reduction in YMRS score from baseline to 12th week (p<0.001) but there was no statistically significant interaction between time and groups (p=0.71). Significant reduction in HAM-D score from baseline to 12th week was observed in both Olanzapine and Risperidone group (p<0.001)...
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