Posted by admin on Apr 30, 2018 in |
The present study explains computational methods to design 3D structure of “Cytochrome P450 4A11” of Homo sapiens enzyme using the sequence available from Uniprot (Uniprot KB – Q02928). Homology modelling study was performed to generate a 3D model of Cytochrome P450 protein. The model was developed by using Modeler 9.17 software tool. The developed model was further docked with already existing drugs using the Autodock 4.2 software. After designing the model molecular docking studies were performed by using Autodock 4.2 with 5 drugs to identify the functional effect of protein. The developed model shows above 91 % of the amino acids in most favoured region. All the drugs show good binding energy and interactions. The compound olanzapine shows highest binding energy of -7.86 kCal/mol with interacting Gly453. These studies provide understanding and interpreting the data produced by these methods. It explains to understand molecular interactions at the active site...
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Posted by admin on Apr 30, 2018 in |
Aim: To gain an insight into patients medications and their experience of self-reported adverse drug reactions. Methods: A cross sectional study was conducted in the Emergency Department of a teaching hospital in the Midlands, United Kingdom from February to March, 2012.The study included patients aged 16 or over and currently on treatment with one or more medications. All patients were questioned on their medication history, indications for their medicines and experience of any adverse drug reactions with their medicines. Results: Of the 341 patients assessed, data from 85 patients was not complete and therefore was not included in the analysis. 256 (75%) of patients were included in the study. 103(40%) patients reported using over the counter medications, mainly paracetamol and non-steroidal anti-inflammatory drugs. 52 (20%) of the patients did not know the reason for taking their medications and 116 (45%) patients were unaware of the adverse effects of their medications. 79 adverse drug reactions were recorded in 62 (24%) patients with 17 patients suffering from multiple reactions. Lack of...
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Posted by admin on Apr 30, 2018 in |
Delonix regia Rafin. belonging to family Fabaceae and subfamily Caesalpinioideae is flowering plant native to Madagascar and East Africa. Delonix regia reported to have anti-diarrhoeal, anti-inflammatory, antioxidant, hepatoprotective and antimicrobial activity. The present study was carried out to establish the pharmacognostical studies, physico-chemical parameters along with preliminary phytochemical screening of petroleum ether, chloroform, ethylacetate, acetone, methanol and aqueous extracts of Delonix regia Rafin. stem bark. The macroscopical and microscopical characteristics of drug powder were studied. The transverse section of stem bark indicated the arrangement of various cells in cork, cortex, phelloderm and pith region. The preliminary phytochemical screening of various extracts revealed the presence of carbohydrate, protein, glycosides, flavonoids, sterols, phenolic and tannin compounds. The physico-chemical parameters such as total ash, acid insoluble ash, water insoluble ash and sulphated ash (8, 2.005, 3 and 1.4 %w/w respectively), loss on drying (45 %w/w) extractive values, foaming index, swelling index and fluorescence analysis of stem bark powder were studied. These studies will be helpful to establish standards for quality, purity and...
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Posted by admin on Apr 30, 2018 in |
A new simple, sensitive, precise, accurate and stability indicating RP-HPLC method has been developed for the simultaneous determination of epalrestat and pregabalin in combined tablet dosage form. The chromatogram was achieved with STD Discovery column 250 × 4.6 mm, 5m and mobile phase containing 0.1% ortho phosphoric acid buffer and acetonitrile taken in the ratio of 45: 55 was pumped through column at a flow rate of 1 ml/min. temperature was maintained at 25 °C. The effluents were monitored at 244 nm by using PDA detector. The retention time of epalrestat and pregabalin were found to be 2.407 min and 3.272 min. The validation of the method was done according to the ICH guidelines for different analytical parameters. The method was found to be linear in the range of 37.5 – 225 µg and 18.75 – 112.5 µg/ml for epalrestat and pregabalin respectively. The assay of marketed formulation was determined and find with 99.22% and 99.07% w/v for epalrestat and pregabalin respectively. The stressed sample was analysed and this...
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Posted by admin on Apr 30, 2018 in |
Objectives: To assess clinical and histopathological patterns, causative drugs, causality, severity and cost of treating cutaneous adverse drug reactions (CADRs) among patients admitted in tertiary care teaching hospital. Methods: In a prospective hospital-based study over a period of one year (June 2015- June 2016), CADRs of patients admitted to the dermatology department were recorded. The data was subjected to descriptive analysis. Results: Of the total 39 cases, 24 (61.54%) were male and 15 (38.46%) were female. Maximum patients (48.7%) belonged to 21 – 40 years age group. On causality analysis, 23 cases (58.33%) were of probable type whereas 16 (41.02%) of possible type. Steven Johnson syndrome (46.15%), maculopapular drug rash (20.51%) and drug reaction with eosinophilia and systemic symptoms (15.38%) were common CADRs. There were total 55drugs which could account for observed CADRs mostly belonging to anti-epileptic (32.72%), anti-microbial (29.09 %) and NSAIDs (21.81%) classes. The most frequent offending drug was phenytoin (18.18%) followed by paracetamol (10.91%) and carbamazepine (7.27%). Maximum CADRs were of severe type (66.67%) but no...
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