Posted by admin on May 31, 2018 in |
For a formulation scientist, the delivery of therapeutic agents to the diseased organ has been a challenging problem. It is expected to improve the therapeutic index and reduce side effects of a drug by good delivery strategy to diseased organ. The objective of this investigation is to evaluate the targeting potential of solid lipid nanoparticle (SLN) formulation of imatinib mesylate to the lymphatic system using response surface methodology of design of experiment. Box-Behnken DOE was constructed using imatinib mesylate, compritol 888 ATO (X1) and pluronic F68 (X2) as independent factors and particle size (Y1) and entrapment efficiency (Y2) as dependant factors. The SLN formulation was prepared by hot homogenization followed by ultrasonication. Prepared SLN were analyzed by FTIR, DSC, DLS, PXRD and SEM. The optimized particle size and entrapment efficiency of the imatinib mesylate loaded solid lipid nanoparticle was found to be 190 nm and 62.5% respectively, which are sufficient to reach lymphatic system. FTIR revealed no interaction between imatinib mesylate and compritol 888 ATO. Compritol 888 ATO retained...
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Posted by admin on May 31, 2018 in |
Analytical study of Ayurvedic preparations is the need of present scientific era. Though the Ayurvedic drugs are time tested and have been used successfully in the management of various ailments, it is now necessary to prove their quality, efficacy and safety to the scientific world through various modern analytical parameters. Chincha kshara is an important preparation mentioned in ayurvedic classics for the management of various diseases. An attempt has been made in the present study to prepare Chincha kshara and analyse the physical nature, surface morphology and chemical constituents present in the Chincha kshara through analytical parameters like organoleptic properties, pH value, X-ray diffraction technique (XRD), scanning electron microscopy (SEM) and energy dispersive X-ray spectroscopy (EDS). XRD study of Chincha kshara showed the presence of potassium carbonate hydrate as major and minor peaks and sodium chloride, potassium calcium hydrogen carbonate phosphate hydrate, silver phosphate and magnesium phosphate oxide as minor peaks. SEM revealed its amorphous nature and EDS reported the significant presence of Potassium (50.44%) and Oxygen (46.07%) in...
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Posted by admin on May 31, 2018 in |
Objective: The present investigation of chemical constituents of Annona squamosa was undertaken as part of a wider study to find out the pharmacological active constituents present in this plant. So in this study, we investigate the pharmacological evaluation of previously isolated compounds (AS1-AS5) from the bark of Annona squamosa. Methods: The anthelminthic and antioxidant potential of the isolated compounds (AS-1 to AS-5) from Annona squamosa were evaluated for in-vitro anthelmintic activity using Indian earthworm Pheretima posthuma and DPPH model respectively. Results: The in-vitro anthelmintic activity results indicated that isolated compounds (AS-4 and AS-5) have good paralysis time comparable with albendazole standard. The antioxidant activity is evaluated for in-vitro antioxidant activity using DPPH radical scavenging activity, AS-5 & AS-4 having IC50 65.77 & 92.97 µg/ml revealed potent antioxidant activity comparable to standard ascorbic acid (IC50 43.42 µg/ml). Conclusion: This study suggests that bark of Annona squamosa have bioactive compounds for a new anthelmintic and antioxidant drug development. Further research on this plants core is needed for the discovery of a...
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Posted by admin on May 31, 2018 in |
Sri Lankan Ayurveda clinical practice Satapushpa Shatavari powder (SSP) with or without Satapushpa Shatavari Grita (SSG) enema is used for manage menstrual disturbances due to polycystic ovary syndrome (PCOS). Objective of Present study was to evaluate the efficacy of Satapushpa Shatavari Churna with Satapushpa Shatavari Grita Matra Vasti for the management of PCOS by an open label comparative clinical trial. Successful screening 60 PCOS participants were randomly divided into three groups (n = 20) viz Group A (Oral Administration of SSP 05g t. d. s. with 10 ml of cows ghee for 2 weeks), Group B (Rectal Administration of SSG as enema, 60 ml / day for 2 weeks with 1 week gap) and Group C (oral and rectal administration of SSP and SSG drugs respectively). After one month administration period participants in group A, B and C were assessed on PCOS appearance of the ovary, volume of the ovary, endometrial thickness by US scan. Further volume of menstrual flow, intermenstrual period and hirsutisum was assessed by standard scoring...
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Posted by admin on May 31, 2018 in |
The effect of four extracts of Solanum chrysotrichum on the proliferation of bacteria, yeasts, mouse spleen cells and human transformed cells was determined. Dry leaves grounded were consecutively macerated 24 h with hexane, chloroform, methanol and water. Dilutions of 1, 10 and 100 µg/mL / DMSO were used for the assays with normal mouse spleen cells, DU-145 and MCF-7 cells. The cellular proliferation was evaluated by the sulforhodamine method´s. Antibacterial and antifungal activities were determined by methods of diffusion and dilutions, using concentrations of 10, 15, 20 and 25 mg/mL. The bacteria used were Escherichia coli SOS, Proteus mirabilis NCTC2896, Staphylococcus aureus ATCC6538 and Bacillus subtilis. The yeasts were Candida albicans, Candida tropicalis and Cryptococcus neoformans. The bacteria were cultured in Agar-Mueller-Hinton and the yeasts in Sabouraud Agar and incubated at 37 and 28 ºC, respectively 24 h. Methanol and watery extracts at dosage assayed caused a cellular concentration reduction average of 56 and 59%, on cells DU-145, respectively. The chloroform extract at doses of 100 µg/mL increased to...
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