Posted by admin on May 31, 2018 in |
The Pharma market is getting flooded with fixed dose combination drugs due to better patient compliance, has but created a challenging situation for analytical chemists. The objective of study was developing an analytical method for simultaneously estimating aliskiren and amlodipine in combination with their forced degradation products obtained by treating the sample under tress conditions like acid and base hydrolysis (0.01N HCl, and 0.01 NaOH, oxidation(3% H2O2), photolytic and thermal degradation(80oC). The linearity ranging was 2.5-25μg/ml and 10-100μg/ml of Amlodipine and Aliskiren and regression coefficient was found to be 0.998 and 0.996 respectively. The chromatographic separation of analyte was carried out using JASCO HPLC system with HiQ Sil C8 (4.6mmØ × 250mm, 5 ?) column. The mobile phase consists of acetronitrile and pH was adjusted to 4 with orthophosphoric acid solution. The analyte were detected at 210nm using UV detector with flow rate of...
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Posted by admin on May 31, 2018 in |
Dipyrone (sodium noramidopyrine methanesulfate), a member of the pyrazolone group, is a drug that possesses analgesic, antipyretic, anti-inflammatory, and spasmolytic properties. Traditionally, iodimetric methods have been recommended for the quantitative determination of dipyrone in all its pharmaceutical forms. This work describes the development of zero order and fourth order (∆λ = 10) derivative spectrophotometric methods for the determination of dipyrone in different pharmaceutical formulations, using water as solvent. The analytical curves presented correlation coefficients greater than 0.999, limits of detection varying from 0.23 to 0.49 mg L-1, and limits of quantification varying from 0.78 to 1.63 mg L-1. The average recoveries ranged from 98 to 103 % for the zero order method and from 96 to 104 % for third and fourth order derivative methods. Intra and inter-day precisions, expressed as relative standard deviations, were below 4.2 %. The excipients present in dipyrone tablets and solutions did not interfere in the analyses. The method was shown to be linear, reproducible, specific, sensitive and...
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Posted by admin on May 31, 2018 in |
Hyperlipidemia is a major risk factor for CHD, atherosclerosis and diabetes. Present study has been conducted to evaluate the antioxidant and anti hyperlipidemic activity of Fragaria nubicola. All in-vitro antioxidant activities were concentration dependent, which were compared with standard antioxidants BHT and Vitamin C. At concentration of 700 µg/ml the DPPH radical scavenging activity of methanol extract was more than that of aqueous and ethyl actetate extracts. The highest phenolic content was observed in methanolic extract followed by aqueous and ethyl acetate. Superoxide and hydroxyl radical scavenging activity of extracts increased in dose dependent manner with highest in methanolic extract. To evaluate hyperlipidemic activity, 35 rats weighing 80-100gm were divided into 7 groups with five in each group and were fed with high fat diet for one month. Group I contained normal receiving water only, group II Hyper- lipidemic receiving high fat diet, group III Atorvastatin (10 mg/kg), group IV and group V receiving methanolic extract of 50 and 100 mg/kg, group VI and VII receiving aqueous extract of...
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Posted by admin on May 31, 2018 in |
The aim and objective of the present work is to design and develop immediate release tablets of pioglitazone hydrochloride to improve the patient compliance and desired bioavailability by selected delivery system by following immediate release mechanism. In the present investigation, super disintegrants used in the combination of 1:1 ratio were modified using co-processed technology with the solvents like acetone and methanol using solvent evaporation technique. The dissolution rate of the drug was significantly increased which reaches closer to the dissolution profile of marketed product. This was due to an increase in surface area of drug available for dissolution. The physical modification of super-disintegrants using co processing technique and selection of processed material with particle size specificity made the drugs to possess required characteristics as fast dispersible. The pre compression and post compression properties are determined as per the procedure prescribed. The drug release studies and kinetics indicate that the prepared tablets with co-processed superdisintegrants were best at its release in the entire tract of GIT due to their change...
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Posted by admin on May 31, 2018 in |
Background: Brachial plexus block provides minimal systemic impairment and excellent localized postoperative analgesia for upper extremity surgery. LA’s such as lignocaine, bupivacaine and ropivacaine are widely used along with adjuvant to improve the quality, onset and duration of block and to decrease postoperative analgesic requirement and systemic side effects. Adjuvant like α-2 agonist (Clonidine), opioids etc. are been added to LA’s. Clonidine is an imidazoline derivative with α-2 adrenergic agonistic activity. There has been seen a potentially clear synergism between clonidine and ropivacaine in various regional block anaesthesia. Material and Methods: In this randomized doubled blind controlled study, a total of 60 ASA grade I or II patients were taken and randomly allocated into 2 groups comprising 30 patients in each group. Group R includes patients which were given 30ml of 0.5% ropivacaine + 2ml normal saline and in Group RC 30 ml of 0.5% ropivacaine with 2 µg/kg clonidine in 2 ml NS. Sensory function was tested using pinprick and motor with concomitant inability to move the wrist...
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