Posted by admin on Jun 1, 2018 in |
By considering the current regulatory requirement for an analytical method development, a reversed phase high performance liquid chromatographic method for routine analysis of cobicistat has been developed using analytical Quality by design approach. The optimized method was achieved using unisol C-18 (3 μm, 4.6 ×150 mm) column with mobile phase consisting of mixture of water and methanol (70: 30 v/v) with a flow rate of 1ml/min at 240 nm. The optimized method was then validated according to the ICH guidelines. The developed method was found linear over the concentration range of 10-80 μg/ml and the detection and quantitation limit was found to be 0.39μg/ml and 1.2μg/ml. There are no interfering peaks under performed degradation conditions. Therefore, a sensitive, robust, accurate and stability indicating method was developed with high degree of practical...
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Posted by admin on Jun 1, 2018 in |
Renal stones disease is the most common disease of the urinary tract affecting about 10% of the global population. Renal stone disease is a common disorder that develops when the urine becomes overly saturated with certain microscopic substances that bind into hardened mineral deposits known as renal stones. The objective of our current study is to evaluate the total organic acids of urine of calcium oxalate stone formers and compare it with that of non stone formers, to investigate whether the difference between the property studied in two groups really exists or not. The study was conducted on 50 patients divided into two groups- Group I consist of 25 idiopathic patients having calcium oxalate renal stone i.e. stone formers (SF) and Group II consists of 25 controls i.e. non stone formers (NSF) having no clinical evidence of renal stones. Each patient and control collected a 24 h urine, which was kept unrefrigerated, using thymol as preservative. It was found from our studies that evaluation of Total organic acids can...
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Posted by admin on Jun 1, 2018 in |
The mineral Srotonjana (antimony sulfide) is being used by Indians since ancient times. In Ayurvedic texts, it has been directed that Srotonjana should be used only after shodhana. Shodhana is a process to remove the unwanted physical, chemical impurities and toxic material and makes it suitable for medicinal use. Metallography is an advanced scientific study which is used in the study of the microstructure and identification of the parent metal particles along with the nature of compound formed during the shodhana. The difficulty of low solubility and less dissolution rate of metal and minerals has received broad academic and industrial awareness. So the aim of present study was to generate metallographic and solubility in the different solvent study as a tool (fingerprint) for ashodhita and shodhita Srotonjana for quality assessment and standardization. In the present study, Srotonjana was shodhit by seven bhavana (levigation) with bhringraj swaras and with triphala kwath. The finding results revealed that after shodhana Srotonjana broke into smaller particles and became homogeneous in both shodhit the...
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Posted by admin on Jun 1, 2018 in |
Helminthiasis is also known as worm infection and a endoparasitic disease of humans and other animals in which a part of a body is infected with parasitic worms. Tinospora cordifolia is a well known medicinal plant and widely used in folk medicine ayurvedic system. The objective of this work is to carry out phytochemical screening and in vitro anthelmintic activity of methanolic, petroleum ether and ethylene glycol extracts of leaves of Tinospora cordifolia (Menispermaceae) against Indian earthworm, Pheretima posthuma. Various concentrations (2.5, 5, 10, 20 mg/ml) of the extracts were tested, which involved determination of time of paralysis and time of death of the worms using Albendazole (10 mg/ml) as standard drug and normal saline as control. The methanolic extract exhibited a maximum anthelmintic activity than the other two extracts. The extract showed anthelmintic activity in dose dependant manner giving shortest time of paralysis (P) and death (D) with 20mg/ml concentration and caused paralysis in 4 min and death in 10 min respectively. The Preliminary phytochemical analysis indicated the...
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Posted by admin on May 31, 2018 in |
Cefepime is a broad – spectrum new parenteral cephalosporin used to treat moderate to severe nosocomial pneumonia, empirical treatment of febrile neutropenia and infections of the skin and urinary tract. In this present study a simple, accurate and precise reverse phase high performance liquid chromatographic method was developed and validated for analysis of cefepime powder for injectable solutions along with its potency by using microbiological bioassay by four different microorganisms. A YMC C 18 (4.6 × 150 mm, 5.0 mm) column was used for cefepime separation, using isocratic elution with acetonitrile: water (70:30, v/v) and UV detection at 235 nm. Microbiological assay (bioassay) was performed using the agar diffusion method. The validation performed yielded good results in terms of linearity, precision, accuracy, and robustness. The retention time obtained for cefepime was 1.77 min and % potency of the marketed dosage form was found to be 147.9%, 100%, 83.17% and 125.8%...
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