Posted by admin on Jan 31, 2018 in |
The purpose of present work was to improve the oral bioavailability of embelin by formulating this hydrophobic phytoconstituent as self emulsifying drug delivery system (SEDDS). SEDDS improves the oral bioavailability by overcoming the dissolution step, enzymatic degradation and hepatic first pass metabolism of phytoconstituent. On the basis of solubility study of embelin, ethyl oleate, tween 80 and span 20 were selected as oil, surfactant and co surfactant respectively for the formulation. Phase diagram study revealed the ratio of surfactant and co surfactant (Smix) for effective self emulsification. Prepared batches of formulation were evaluated for various physicochemical parameters which includes thermodynamic stability study, emulsification time, morphology study of droplets, determination of zeta potential, viscosity determination, percentage drug content, long term stability study. Pharmacokinetic study of plain drug, marketed tablet and SEDDS of embelin was also done which includes in vitro drug release study which revealed that the pattern of drug release is almost uniform and constant moreover embelin SEDDS have highest percentage cumulative drug release (93%) as compared to the...
Read More
Posted by admin on Jan 31, 2018 in |
Introduction: To assess the level of knowledge, attitude, and practices of women of reproductive age of 20 – 70 years on their understanding of the risk factors of cervical cancer, its early detection, and prevention. Methods: A cross-sectional study was conducted in rural areas of Komarapalayam from August 2016 to January 2017 by using pre-tested modified questionnaire. The purpose of the questionnaire was to assess the demographic data, exposure to risk factors, knowledge, attitude, and practice of the participants toward cervical cancer. Results: A total of 500 married women have participated in this study. Majority 26.6% had the secondary education; most common 34% age group being 31 – 40 years. 39% had first childbirth and 34.6% were married between 19 to 22 years; 27.8% had abnormal uterine bleeding were the most common prevalence of risk factors. Mostly 98.4% and 96.6% were heard about cervical cancer and its screening. Many women 82.2% had never undergone cervical cancer screening and 98.9% of the women had never been vaccinated. The main sources...
Read More
Posted by admin on Jan 31, 2018 in |
A novel Mannich base ligand 1-(2,5-dioxopyrrolidin-1-yl) (4-hydroxy phenyl)methyl)thiourea (PSTU) and its Manganese (II) and Cobalt (II) complexes have been synthesized and characterized by elemental analysis, molar conductance measurements, magnetic susceptibility measurements, FT-IR, 1H NMR and 13C NMR spectroscopic studies. The antimicrobial activities and antioxidant activities of the free ligand and its metal complexes were also performed. The results revealed enhanced antimicrobial activities against Bacillus subtilis (gram+ive) and Escherichia coli (gram–ive bacteria) and also significant antioxidant activity. In addition, DNA binding properties of the ligand and its metal complexes have been investigated by UV-Visible absorption spectroscopy, viscosity measurements, emission spectra and gel electrophoresis, which suggest that the metal complexes act as efficient DNA binders. The results shown that the nuclease activity Co(II) complex was effective which could induce scission of pBr322 super coiled DNA effectively to linear form in presence of H2O2 as oxidizing...
Read More
Posted by admin on Jan 31, 2018 in |
The Hypoxic Ventillatory Response (HVR) at high altitude was hypothesized to cause pharmacokinetics changes, especially metabolism of drugs due to the up regulation of CYP3A4 isoenzyme. These pharmacokinetic alteration could affect pharmacotherapy, probably lead to either sub therapeutic or supra therapeutic effects. This hypothesis was studied with a pop-PK study conducted in healthy male volunteers inhabited at geographically high altitude area. Firstly, the HVR was assessed by changes in partial pressures of blood gases, oxygen concentration in arterial blood, oxygen gradient in arterial-alveolar gas exchange and oxygen concentration of arterial blood. Secondly, the Probe drug Alprazolam 1mg oral single dose was given to find out the dysregulation of CYP3A4 and blood samples were analyzed for pharmacokinetic changes The pharmacokinetic variables such as Cmax, Tmax, AUC, Kel, MRT, t½ etc were significantly altered in study subjects. The Cmax and AUC values were significantly reduced in HVR group than non HVR group (21.26 ±1.40, p<0.05). The elimination and clearance were significantly increased in hypoxia group (p<0.05) whereas the Vd was not...
Read More
Posted by admin on Jan 31, 2018 in |
Orchidaceae, one of the largest families of plant kingdom, possesses high aesthetic, medicinal and economic value. However, medicinal properties of orchids has not been investigated to its fullest potential. Hence, an orchid of Arunachal Pradesh, Pleione maculata, which has not been studied from pharmacological and phytochemical view point so far, is selected for the study. The present study was conducted for antibacterial and antimycobacterial activity by bioassay guided fractionation. The whole plant material was divided into diethyl ether (Et2O), n-butanol (n-BuOH) and aqueous (Aq) fractions. The antibacterial activity was evaluated by agar well diffusion method against three Gram negative and two Gram positive multi drug resistant clinical isolates. The antimycobacterial activity was performed by Colorimetric redox indicator assay against H37Rv strain of Mycobacterium tuberculosis. The screening result identified Et2O fraction as the most active fraction showing significant zone of inhibition against E.coli and Staphylococcus aureus sp. and moderate antimycobacterial activity (MIC value 104.16 μg/mL) against H37Rv strain of Mycobacterium tuberculosis. Phytochemical screening of the fractions revealed the presence of...
Read More
