Posted by admin on Jul 31, 2024 in |
Secondary metabolites found in various plants play an important role in curing various diseases and are used as important raw materials for the production of traditional and modern medicine. One of these plants, Paspalum conjugatum, a member of the Poaceae family, has been used for different purposes since prehistoric times. In this research work, we extracted the powdered leaves of P. conjugatum by the sequential extraction method using four different solvents (n-hexane, chloroform, ethanol, and water). About eight phytochemical analyses were carried out, out of which four phytochemicals (alkaloid, tannin, flavonoid, and glycoside) were significantly identified in both n-hexane and ethanol extracts. The proximate analysis was carried out for the leaves powder. The moisture content, total ash, acid-insoluble ash, and water-insoluble ash were found to be 3.56, 9.76, 3.22, and 27.08%, respectively. Reducing power assay, total antioxidant activity, total phenolic content, total flavonoids, and reduction of ferric ions were also investigated as a part of the antioxidant test. All extracts showed a significant presence of antioxidants. The ethanol extract showed...
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Posted by admin on Jul 31, 2024 in |
Vernal keratoconjunctivitis (VKC) is a chronic inflammatory condition affecting the cornea and conjunctiva of young children. Health-related quality of life (HRQoL) is impaired in children with VKC. Mast cell stabilizers are often offered as the first-line therapy for VKC. In this study, the effects of two types of mast stabilizers on HRQoL were compared.This was a prospective, open-label, randomized, and comparative clinical study. Patients were randomized to receive one of two mast cell stabilizers-bepotastine or olopatadine. The HRQoL was assessed using the Quality of Life in Children with Vernal Keratoconjunctivitis (QUICK) questionnaire at baseline and after 3 weeks. The change in the QUCIK score was recorded to see the impact of treatment on HRQoL. The mean age of the cohort was 9.6 ± 2 years. Most patients presented in the months of April and May. A statistically significant (p <0.1) change of over 60% in QUICK scores was observed in both groups, but there was no statistically significant difference (p 0.07) in the QUCIK questionnaire score at 3 weeks...
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Posted by admin on Jul 31, 2024 in |
Introduction: Aceclofenac (ACE) and Curcumin (CUR) are anti-inflammatory drugs utilized for osteoarthritis and rheumatoid arthritis. ACE, As NSAID inhibits prostaglandins, while CUR, a natural polyphenol, exhibits antioxidant and anticancer properties. Combining them may offer synergistic benefits for inflammatory diseases. Objective: To develop a UV spectrophotometric method for simultaneous determination of ACE and CUR from a developed dosage form, focusing on simplicity, Accuracy and cost-effectiveness. The method employs absorbance correction to calculate drug concentrations based on their absorbance difference at two wavelengths. Method: Using a solvent of phosphate buffer pH 5.5: ethanol (1:1), standard solutions of ACE (5-30 µg/mL) and CUR (1-7 µg/mL) were prepared. UV spectrophotometer measured absorbance at 276 nm and 430 nm for both standard and sample solutions. Absorbance correction equations were used for concentration determination. Result: Method validation followed ICH guidelines, demonstrating good linearity (correlation coefficients: 0.999 for ACE, 0.998 for CUR), accuracy (recovery values: 92.72% for ACE, For cur) and precision (RSD < 2% for intra-day and inter-day variations). Specificity was confirmed, and method robustness...
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Posted by admin on Jul 31, 2024 in |
Shilajit, a herbo-mineral blackish brown resin, extracted from humic rocks, is attributed to several therapeutic claims in Ayurveda like antiaging, health-promoter, immune- booster, urogenital problems and bio-enhancer. The lack of strong parameters for its quality control and standardization; and the knowledge gap to explain its mechanism of action are the important factors behind its restricted use. Here, for the first time, we have used a High-Resolution Advance Mass Spectrophotometer (HRAMS) to visualize the metabolites of shilajit and further identified novel metabolites for its characterization. Seven brands of shilajit, available in the market (5 resins and 2 capsules), were purchased and used to validate our findings in terms of molecular weight, abundance, retention time and structural characteristics. We found 14 novel metabolites and six of them were acidic, eight were non-acidic. Among them, only 4 metabolites were common in all seven samples, which were (a) NP-020214, (b) 3-tert- Butyladipic acid, (c) Dodecanedioic acid and (d) Azelaic acid. However when only resins were compared, then additional nine common metabolites were identified...
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Posted by admin on Jul 31, 2024 in |
Introduction: Self-medication is becoming more and more common in our daily lives but it’s an unhealthy and risky habit. It assumes that second year MBBS students holds special significance as they start to deepen their knowledge about diseases and medications, which forms a critical foundation for their medical education and future practice. This is the only study conducted in the northeastern region of India till date. The objective of the study is to assess the knowledge, attitudes, and practices regarding self-medication among second-year MBBS students at Silchar Medical College and Hospital. A cross-sectional survey was conducted using a pre-formed questionnaire including both open and close ended questions among the second year MBBS students over a period of one month after taking written inform consent from the participants. Data was collected by using google form and result expressed as counts and percentage. Result: Out of 120 participants, 72(60%) were males and 48(40%) were females. Out of them total 116 students took self-medication and among them 90 students provided reasons in...
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