Posted by admin on Feb 29, 2024 in |
Moringa oleifera is one plant that is multi-purposefully used for the prevention and treatment of numerous diseases. This study analyzed the phytochemical and elemental contents of M. oleifera leaf extract, and evaluated its effect on fibroblast proliferation, which is crucial for wound healing. M. oleifera leaf samples were collected, identified, and extracted using solvents of different polarities. High-performance thin-layer chromatography (HPTLC) and ultra-high-performance liquid chromatography (UPLC) were employed to analyze the phytochemical composition, while inductively coupled plasma-optical emission spectrometry (ICP-OES) was used to determine the elemental content. The effect of the hydroethanolic extract was investigated on proliferation of Hs27 fibroblast skin cells by the MTT assay. Water extraction yielded the highest extract of 13.0±3% w/w, while petroleum ether extraction yielded the least of 3.03±0.8% w/w. HPTLC fingerprinting of the 50% hydroethanolic extract identified bioactive compounds including flavonoids, phenol carboxylic acids fatty acids, and sterols. UPLC analysis confirmed the presence of rutin and kaempferol in the extract. Elemental analysis showed a high concentration of Ca, K, Br, Mg, and P...
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Posted by admin on Feb 29, 2024 in |
Introduction: Topical corticosteroids are mainstay of treatment for many skin conditions but extensive prescription of them may lead to increase in adverse reactions. So, periodic audit of prescriptions will help in enhancing the therapeutic effectiveness and reduce the adverse effects. Objective: To study sociodemographic data and drug prescription pattern in patients with skin diseases. Methodology: Prospective observational drug evaluation type of study conducted at tertiary care teaching hospital during January to December 2018. Secondary data analysis was conducted of 500 prescriptions of dermatologists which were selected randomly in semi structured proforma. Data was analysed as counts and percentage. Results: Total 1551 drugs were prescribed to 500 patients visiting dermatology Outpatient department. Most frequently in 180 prescriptions, 3 drugs were prescribed. Antiallergic are most frequently prescribed drugs (24.3%) followed by Steroids (15.7%). In almost all prescriptions Generic name of drugs, Strength and Duration of treatment were mentioned. Among prescribed steroids topical corticosteroids were prescribed in 69.1% of prescriptions and in topical corticosteroids Clobetasol propionate forms more than half part (53.3%)...
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Posted by admin on Feb 29, 2024 in |
Normally and often skin infections present therapeutic challenges to practitioners and researchers due to the growing concern regarding multi-drug resistant bacterial, Viral, and Fungi Strains. The Antibacterial properties of zinc sulphate and copper sulfate are well known and have been investigated for many previous years. However, the synergistic effect between both metal ions as antibacterial ingredients has not been evaluated in topical formulations like gels and creams. The aim of the present study to formulate cream and gels containing zinc and copper individually or in combination and evaluate the in-vitro antibacterial activity of these metal ion in the formulation. Zinc sulfate and copper sulfate had a strong synergistic antibacterial activity in cream and gels. By evaluating the various parameters such as pH, Spreadability, viscosity, and antibacterial properties it is confirm that the metal gel and cream enhance the antibacterial activity. This study evaluated and confirmed the synergistic in-vitro antibacterial effect of copper sulfate and zinc sulfate in cream and...
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Posted by admin on Feb 29, 2024 in |
Nowadays, many clinical studies have provided reliable results on the effects of several herbal medicines on many diseases such as kidney problems. The objective of this study is to evaluate in-vitro the antiurolithiatic activity of polyphenols, flavonoids, and alkaloids fractions of the leaves of the species Ziziphus lotus. The quantification of the polyphenol and flavonoid fractions was carried out by spectrophotometric methods; on the other hand, the alkaloid fraction is quantified gravimetrically. The antiurolithiatic activity of the fractions is studied by the use of the turbidimetric and gravimetric techniques. The polyphenol content varies between 1198,696 ± 99,848 and 15270,751 ± 290,419 (μg GAE)/g). While flavonoids range from 97,340 ± 8,437 to 1745,864 ± 8,690 (μgQE/g). On the other hand, the alkaloid fraction represents 1. 26%. The polyphenols fraction showed a higher inhibition 89.80 ± 0.69 than the two other fractions which represent the values 84.17 ± 1.11 and 78.90 ± 1.40, respectively. The polyphenols fraction (AF: 86.66 ± 1.43) and flavonoids (DMF: 80.85 ± 2.03) show an increasing evolution...
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Posted by admin on Feb 29, 2024 in |
Production of nanoparticles using plants is a new area of interest. Plum fruit extract was used for the biosynthesis of zinc oxide nanoparticles. Green synthesis of zinc oxide nanoparticles are considered as a eco-friendly methodusing materials from plants and other natural sources without using anyharmful chemicals. Plum fruit has many medicinal values such as antioxidant activity, cardiovascular activity, anti-inflammatory and antimicrobial effect. Present paper discuss about the green synthesis of zinc oxide nanoparticles using Prunus domestica (plum) fruit extract, Its characterization by analytical studies, antimicrobial and antioxidant studies. Plum fruit extract also subjected to phytochemical screening. The active constituents present in Plum were identified as alkaloids, glycosides, phenolics, flavonoids, proteins and carbohydrates. Nano particles were characterized by UV-Visible Spectroscopy, FT-IR and SEM studies. In the UV visible spectrometer absorption peak was observed at 362 nm, which is specific for zinc oxide nanoparticles. Synthesized zinc oxide nanoparticles were subjected to FT-IR analysis to detect the various characteristic functional group associated with the synthesized nanoparticles. The peaks indicate the characteristics functional group...
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