Posted by admin on Apr 30, 2024 in |
The increasing resistance of microorganisms to conventional antibiotics has prompted scientists to explore alternative sources for antimicrobial compounds. This study focuses on Curcuma amada, a perennial herb, and its rhizome extracts for antibacterial activity against major bacterial strains identified in burn wounds: Escherichia coli, Staphylococcus aureus, Klebsiella pneumoniae, and Pseudomonas aeruginosa. Various extraction methods like Cold extraction, Microwave Assisted Extraction and Soxhlet Extraction were employed to obtain ethanolic and aqueous extracts and their effectiveness was evaluated through Minimal Inhibitory Concentration (MIC) testing. MIC, crucial in determining antimicrobial potency, revealed that the extract inhibited visible bacterial growth at a concentration of 100μg/ml for all tested organisms. This concentration was identified by a colour change from violet to pink, signifying the absence of observable microbial growth. Diverse medicinal properties of Curcuma amada, including anti-inflammatory, digestive, and febrifuge effects, present a promising avenue for developing natural antimicrobial agents. The findings suggest that these plant extracts possess potential as safe alternatives or complementary treatments in managing burn wounds. This research highlights the importance...
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Posted by admin on Apr 30, 2024 in |
Background & Aims: Laparoscopic surgeries produces significant hemodynamic changes leading to increased opioid consumption. This study aims to assess the efficacy of a unique opioid free multimodal analgesia in the prevention of adverse hemodynamic changes during intubation and pneumoperitoneum in a patient undergoing laparoscopic surgery in comparison with standard opioid based anaesthesia. Material & Methods: Sixty patients undergoing elective laparoscopic surgery were randomly assigned into two groups Group OFA (Opioid Free Anaesthesia) received tablet pregabalin 150mg 1hour prior to induction. Intravenous ketorolac 30mg, magnesium sulphate 30 mg/kg, preservative free lidocaine 1.5mg/kg, dexamethasone 8mg was administered as an analgesic mixture in 100ml normal saline half an hour prior to induction. Dexmedetomidine 1mcg/kg diluted in 100ml saline was given over 10minutes prior to induction. Group OBA (Opioid Based Anaesthesia) received placebo tablet 1hour prior to induction,100ml normal saline half an hour prior to induction and 100 ml saline was given over 10minutes prior to induction. The primary outcome was to compare the efficacy of hemodynamic suppression to intubation and pneumoperitoneum. Other...
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Posted by admin on Apr 30, 2024 in |
Acetaminophen (APAP) is the most consumed potentially toxic drug in the world, and its cardiovascular injury has been emphasized. We evaluated the role of inflammatory mediators in the impaired vascular relaxation after APAP treatment. Rats were treated with APAP for 2 weeks (400 mg/Kg/day/p.o.) and after euthanasia, blood was collected for biochemical analysis (hepatic transaminases, lipid peroxidation and glutathione), and aortas were isolated for vascular reactivity, lipid peroxidation and biochemical analysis (glutathione, arachidonic acid, TBXA2, PGD2, 12-HETE and 15-HETE). Both blood and arteries presented increased levels of lipid peroxidation and decreased levels of glutathione. The vasodilation response to acetylcholine was impaired in the APAP group and restored after treatment with diclofenac (10 μM). In the arteries, levels of arachidonic acid were reduced while PGD2 was increased (TBXA2, 12- and 15-HETE remained significantly unchanged). Thus, oxidative stress and contractile prostanoids play a significant role in the impaired vascular relaxation caused by APAP...
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Posted by admin on Apr 30, 2024 in |
An easy, precise and accurate spectroscopic technique for the simultaneous estimation of nicotinamide and metronidazole in semi-solid dosage form and in bulk form has been developed. The present method involves the addition of 0.1M hydrochloric acid solution, followed by dilution with water and subjecting the resulting solution to UV spectroscopic assessment. Absorption maximum was found to lie at about 262 nm and 317 nm for nicotinamide and metronidazole respectively. The calibration curve showed linearity as per line equation y=0.0029x + 0.0001 with an R2value of0.9937 for nicotinamide and y = 0.0072 x + 0.0063 with an R2 value of 0.9933 for metronidazole. Validation was performed as per ICH guidelines for linearity, accuracy and precision. The assay result was in a good arrangement with the label claim. The mean Sandell’s sensitivity values for metronidazole and nicotinamide, respectively, were 0.354 g/cm2 and 0.134 g/cm2, showing that the approach is very sensitive and practical for measuring both...
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Posted by admin on Apr 30, 2024 in |
A simple, rapid, precise, sensitive and reproducible Reverse phase high performance liquid chromatography (RP-HPLC) method has been developed for the quantitative analysis of Tepotinib in presence of its impurities (1&2) in pharmaceutical dosage form. Chromatographic separation of Tepotinib and its known impurities were achieved on Waters Allianace-e 2695 by using Inertsil ODS C18 250×4.6mm 5µ column and the mobile phase containing (0.01M) KH2PO4 PH-2.5&Acetonitrile in the ratio of 50:50% v/v. The flow rate was 1.0ml/min; detection was carried at 263nm using a photodiode array detector at ambient temperature. The calibration range was 10-60µg/ml for Tepotinib, 2.5-15µg/ml for Imp-1& 3.75-22.5µg/ml for Imp-2. %Relative standard deviation of peak areas of all the measurements less than 2%. Recovery was obtained between 98-102%. Stability studies were also performed with known impurities the drug showed maximum degradation in alkaline stress condition. The proposed method was validated according to ICH guidelines. The results obtained were within the acceptable limits. The proposed method was found to be simple, economical, precise, accurate & robust for quantitative analysis...
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