Posted by admin on Apr 30, 2018 in |
Bimetallic nanoparticles have several useful opto-electronic and catalytic properties that are different from those of monometallic nanoparticles. The development of non-toxic and eco-friendly protocols for the synthesis of bimetallic nanoparticles is a still less explored area. In the present study, bimetallic silver-gold nanoparticles were synthesized using Trichoderma reesei (NCIM 992). An effective approach for co-reduction of metal salts was adopted wherein the fungal biomass was grown under non-limiting conditions, followed by the addition of metal salts. The bio fabricated bimetallic nanoparticles were characterized using UV-Vis Spectroscopy, ICP-MS, SEM equipped with an EDS, FTIR and XRD. The fungal biomass color changed from colorless to dark purple within 2 days of incubation period. UV-Visible spectrum of the intracellularly produced bimetallic nanoparticles showed characteristic peak between 420 nm and 540 nm. ICP-MS of the residual salt concentration depicted the percentage of bioconversion of metal salts to metal nanoparticles. SEM-EDS studies confirmed the presence of intracellular silver-gold bimetallic nanoparticles. FT-IR analysis revealed the existence of peaks in the amide regions, a characteristic of...
Read More
Posted by admin on Apr 25, 2018 in |
Hydnora africana Thumb (Hydronaceae) is a parasitic flowering plant with significant global ethnomedicinal value. This study evaluated the toxicological effects of the acute and sub-acute dosing treatments of the aqueous root extract of Ha in male and female wistar rats using OECD guidelines 423 and 407. For the acute study, the extract was administered to the animals at single oral graded doses of 1000, 2000 and 5000 mg/kg body weight (b.w.) and subsequently observed for 14 days, while it was given at 250, 500, and 1000 mg/kg b.w. once daily for 28 days in the sub-acute testing. Clinical toxicity signs, behavioral changes, hematological and biochemical parameters were then monitored and evaluated. The result revealed that, at a limit dose of 5000 mg/kg b.w., the extract neither had treatment – mediated symptoms of toxicity nor mortality over the 14 days observation period. In the sub-acute study and particularly for the male rats, the extract significantly increased the serum levels of the erythrocytes and haemoglobin, while the platelet counts increased dose-dependently...
Read More
Posted by admin on Apr 21, 2018 in |
This is a simple, economic, sensitive stability indicating RP-HPLC method for the simultaneous estimation of metformin and teneligliptin in bulk and pharmaceutical formulation. The method was carried out on octa-decyl C18 column (5 μm, 25 cm × 4.6 mm, i.d) using methanol: water in the ratio of 70:30 and pH of the mobile phase up to 3 was adjusted with OPA at a flow rate of 1.0 ml/min. The wavelength for metformin and teneligliptin at 235 nm was found to be appropriate. The linearity range was obtained in the concentration range of 25 – 150 μg/ml for MET, while 5 – 30 μg/ml for TNG respectively. The retention time of metformin and teneligliptin was found to be 2.45 and 6.68 min, respectively. The regression equation for MET and TNG were found to be as y = 8.288 x + 0.026 and y = 27.26 x + 38.28 with correlation coefficient (R2) 0.999 and 0.999, respectively. The developed method was found to be robust, accurate and sensitive which can be...
Read More
Posted by admin on Apr 9, 2018 in |
Pharmacological activities have been reported for the seed extracts of Lepidium sativum L.; however, the plant has not been widely studied for its effects on the thyroid gland. Objective: The present study was designed to investigate to possible potential pharmacological activity of ethanolic extract of Lepidium sativum L. seeds on regulation of thyroid hormone concentrations in male rats. Methodology: Each experiment was performed on Male Wistar rats, which were randomly divided into 4 groups. Group I received normal saline for 21 days and served as normal control. Group II received extract seeds of L. sativum at a dose of 200 mg/kg i.p. for 21 days. Groups III was treated with a 25 μg/100 g intraperitoneal (i.p.) T3 injection for 21 days and; Group IV was treated iopanoic acid (IOP; 100 mg/kg) for 21 days. Results: The levels of the rats’ serum Triiodothyronin (T3), Thyroxin (T4) and Thyroid-stimulating hormone (TSH) were analyzed. Thyroid gland specimens were processed for microscopic examination. Group II treated with extract seeds of L. sativum showed significant decline T3...
Read More
Posted by admin on Apr 5, 2018 in |
Oral films are a novel approach for the development of orally disintegrating dosage forms. They are thin, elegant in appearance and can be made into various sizes and shapes like rectangular or circular. The strips may be flexible or brittle, opaque or transparent. Oral films are made up of various hydrophilic and hydrophobic polymers to provide rapid disintegration on the tongue without the need for water. The aim of the present study is to develop fast dissolving films of tramadol Hydrochloride by enhancing drug dissolution in the oral cavity. The development of the films decreases the analgesic effect in less time and increases the patient’s compliance. Tramadol HCl films were prepared using Hydroxy propyl methyl cellulose-E5, Hydroxy propyl methyl cellulose-E6, Hydroxy propyl methyl cellulose-E15 and sodium alginate as polymers. Therefore the prepared films were evaluated for various parameters like physical appearance and surface texture of the film, thickness of the film, folding endurance, moisture uptake, uniformity of weight and drug content, swelling index, in vitro disintegration studies, and in...
Read More
