Posted by admin on Jan 31, 2018 in |
T-cell activation, with the low expression of CD62L (L-selectin) marker in the spleen, will increase B cell activity to produce lupus antibodies. Therefore, lupus patients need drugs which could increase the L- selectin expression in the spleen to reduce the auto-reactive T cell. The tested drug to increase the L-selectin in this research was the ethyl acetate fraction of Kalanchoe pinnata (Lmk) Pers leaves (EF-KP), based on its immunosuppressant and anti-inflammatory activities. The research was performed by means of 2, 6, 10, 14 tetramethylpentadecane (TMPD) induced lupus-like mice. The experimental groups consisted of negative control (NC), EF-KP, and positive control (PC) groups. After 21 day treatment, the blood leukocyte was checked. Then, the spleen was prepared for the flow cytometry method to measure CD4+CD62L+ and CD8+CD62L+ T cells. The relative percentage of CD4+CD62L+ T cells in EF-KP groups increased insignificantly (p>0.05) (NC=7.91 ± 4.98%, EF-KP = 8.63 ± 3.07%, PC = 12.62 ± 8.09%). The relative percentage of CD8+CD62L+ T cells increased significantly (p < 0.05) (NC = 6.20...
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Posted by admin on Jan 31, 2018 in |
Elaeocarpus ganitrus Roxb. is reported to exhibit multifarious pharmacological activities. However no scientific study has been conducted to evaluate antiatherosclerotic /lipid lowering activity of E. ganitrus Roxb. The present study was designed to investigate the antihyperlipidaemic and antioxidant activity of 70% ethanolic extract of E. ganitrus seed (EGEE) in cholesterol fed hyperlipidaemic rabbits. The EGEE was administered at a dose level of 250 and 500 mg/kg/day (p.o.) for 60 days to hyperlipidaemic rabbits. Lipid profile in serum and antioxidant parameters in tissues (Liver, Heart and Aorta) were determined. The statistical analysis was carried out using one way ANOVA followed by Tukey’s multiple comparison tests. EGEE showed a decrease in the levels of serum total cholesterol, triglycerides, phospholipids, LDL, VLDL (P ≤ 0.01, ≤ 0.001) in a dose dependant manner in treated animals. HDL ratio improved profoundly as well as marked decline was noticed in atherogenic index after administration with EGEE. A considerable decrease in lipid per oxidation and a significant elevation in Glutathione, Catalase and SOD levels (P ≤...
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Posted by admin on Jan 31, 2018 in |
The research investigated the modification of nano-emulsion by using transesterfied soyabean oil, to enhanced solubility and diffusion of voriconazole. Ternary phase diagrams of soyabean oil, acid / base esters of soyabean oil, Tween 80: PEG 400 (S/CoS) and water were developed. Ester value of soyabean oil increased from 214.04 to 261.5mg KOH/1g for acid ester and 293.58mg KOH/1g for base ester confirmed transesterification. The modified nanoemulsion batches E4, E5, EA4, EA5, EB2 and EB3 showed thermodynamic stability. Nanoemulsion of Soyabean oil (E3 and E4), acid ester (EA4 and EA5), and base ester (EB2 and EB3) were selected for in-vitro drug diffusion studies. The globule size of batch E4, EA4 and EB2 nanoemulsion was found to be 185.6, 162.8 and 115.4 nm, respectively. Zeta potential of batch E4, EA4, and EB2 was observed -35, -27 and -9mV respectively. The in vitro release release profiles from the E4, EA4 and EB2 batches of nanoemulsion gel were fitted into Peppas kinetic model. Moreover, ‘n’ value of batch E4, EA4, and EB2 was...
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Posted by admin on Jan 31, 2018 in |
The objective of the present study is to develop and validate a novel stability indicating UV spectrophotometric method for estimation of Benzydamine Hydrochloride (BNZ) in pharmaceutical dosage form as not a single UV spectrophotometric method has been reported for the estimation of Benzydamine Hydrochloride, which makes the present work novel. In the present research work, UV spectrophotometric determination was carried out at an absorption maximum of 305.6 nm in pH 6.8 phosphate buffer. The purposed method was validated statistically for linearity, accuracy, precision, repeatability, ruggedness, sensitivity as per International Conference on Harmonization guidelines. The drug substance was exposed to acid hydrolysis, alkaline hydrolysis, oxidative degradation, photolytic degradation, and thermal degradation and the stressed samples were analyzed by the proposed method to demonstrate the specificity of the method. The application of developed method was established by performing the assay of pharmaceutical formulation. The proposed method obeyed beer lambert’s law in the concentration range of 5 – 50 µg ml-1 with correlation coefficient (r2) of 0.999. Percentage relative standard deviation associated...
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Posted by admin on Jan 31, 2018 in |
Reverse Phase – High performance liquid chromatographic (RP – HPLC) method was described for determination of Pseudoephedrine Sulphate (PSE) and Loratadine Hydrochloride (LOR). The chromatographic separation was achieved using mobile phase mixture of Acetonitrile: 0.05 M Potassium Dihydrogen Phosphate Buffer: (pH 2.8 adjusted with Ortho Phosphoric Acid Solution): Methanol in the ratio of 30:35:35 (%v/v/v) with column Zorbax Eclipse XDB C18, (150 x 4.6 mm i.d), Particle size 5 mm at 1.2 ml/min flow rate. 10 µL of standard preparation containing 150 µg/ml PSE and 6.25 µg/ml LOR was injected into the column and the component was separated by carrying out elution for a run time of 10 minutes and detected at 254 nm wavelength. The described method shows excellent linearity over a range of 120 to 180 μg/ml and 5 to 7.5 μg/ml for PSE and LOR, respectively. PSE and LOR were subjected to stress degradation conditions of hydrolysis (acid and base), oxidation, Heat and UV light degradation. Stressed samples were analysed by the developed method. The proposed...
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