Posted by admin on Dec 31, 2017 in |
An herbal formulation composed of Allium cepa (Onion), Zingiber officinale (Ginger), Allium sativum (Garlic), Aloe vera, Andrographis paniculata (King of bitters), Allium ascalonicum (Shallot), reportedly used as anti–inflammatory agent was investigated. This study evaluated the physicochemical, phytochemical properties and in vivo anti–inflammatory effect of the herbal formulation. The herbal formulation (Hp-01) was subjected to physicochemical analysis; visual and organoleptic test, thin layer chromatography (TLC), pH, trace elements, phytochemical screening, antioxidant activity using DPPH inhibition and total phenolic acid (TPA) content determination. The biological effect at three doses (half, normal and double) on acute inflammation using carrageenan-induced paw oedema, weight, haematological parameters and liver functions were investigated in Wistar rats. The herbal formulation (Hp-01) was an acidic (pH 3.18), bitter preparation with pungent odour and sediments. The TLC analysis showed a maximum of three spots. Trace elements content of Pb, Cd, Fe, and Zn were within acceptable limits at 0.053, 0.003, 1.190 and 4.190 mg/l respectively. Phytochemical screening showed the presence of anthraquinones, tannins, terpenoids, saponins (2.12%w/w), alkaloids (2.45%w/w) and...
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Posted by admin on Dec 31, 2017 in |
The present study was Rifampicin-resistant (Rif r) mutants of Pseudomonas fluorescens pf2T2 and pf2m1 were isolated from different localities of onion plant rhizosphere soil in an around the Tamil Nadu. However, the mutants of P. fluorescens were of two general categories. Group 1 showed fast growing in terms of growth rate, competitive fitness, and membrane protein composition. Group 2 showed a slower growth rate in both minimal and enriched media and an altered membrane protein profile. These mutants also demonstrated decreased competitive fitness compared with the wild-type strain based on the study of colony morphology, soil characters, different temperature, pH, NaCl (salt tolerant), and gram staining has been done. Although, the Rifampicin – resistant (Rif r) mutants of Pseudomonas fluorescens pf2T2 and pf2m1 were distinctly in the...
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Posted by admin on Dec 31, 2017 in |
Pyrazoline are reduction product of pyrazole. They are well known nitrogen containg 5-memberd heterocyclic compound with broad spectrum of biological activities such as antimicrobial, anti-inflammatory, anticancer, analgesic, anticonvulsant, anthelmintic, antioxidant and herbicidal. Pyrazoline derivatives have been synthesized from intermediate chalcone. Pyrazoles have been the recent target of numerous methodologies, mostly due to their prevalence as scaffolds in synthesis of bioactive compounds and reactions in different media. Fish are the widely used vertebrate organism in risk assessment and regulation. Out of them adult zebrafish and zebrafish embryo has a special characteristic which permits its use as a model organism in toxicological and pharmacological studies. Zebra fish known as a Danio rerio have risen as effective and powerful models for studying developmental processes and human disorders. Recently the zebrafish based assays have been developed for testing toxicity of drug candidates, including acute toxicity (LC50), organ specific toxicity and developmental toxicity. In present article the toxicity of newly synthesized pyrazoline derivatives has been studied on adult zebrafish and zebrafish embryos according to...
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Posted by admin on Dec 31, 2017 in |
In the present study anti-inflammatory activity of ethanol extract of Beloperone plumbaginifolia whole plant were investigated. The anti-inflammatory activity of ethanol extract of whole plant of B.plumbaginifolia were evaluated by carageenan induced rat paw edema to determine its effect on acute phase of inflammation models in rats. The phytochemical screening of ethanol extract of whole plant of B. plumbaginifolia revealed the presence of alkaloid, catechin, coumarin, flavonoid, phenol, tannin, saponin, steroid, glycoside and terpenoid. Maximum inhibition (78.63%) was obtained at the dose of 400 mg/kg of B.plumbaginifolia whole plant after 3h of treatment in carrageenen induced paw edema, whereas indomethacin produced 77.34% of inhibition. The present study suggested that B.plumbaginifolia whole plant extract possess strong anti-inflammatory...
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Posted by admin on Dec 31, 2017 in |
A study was conducted to investigate the effect of different pH, on the growth and biopigment accumulation of a green alga Dunaliella salina isolates from Sambhar salt lake, Rajasthan (India). In order to monitor the impact of pH an algal samples of D. salina was exposed to different pH regimes (6 -10 pH) along with control (8.5 pH) over a period of 30 days. Set of experiments was conducted to study and evaluate the optimum growth and biopigment content for D.salina. The results of this experiment showed that optimum growth of D.salina was found at pH 8. Total chlorophyll content of the algal species decreases as the pH value was increased. While total carotenoids content of the algal species increases as the increases pH values and it was found that the green alga species changed its appearance from green to deep orange. The results indicate that algal species showed diverse response to pH...
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