Posted by admin on Jan 31, 2018 in |
As science encourage most of the medicinal plants as supreme source of unlimited chemical compounds which are of great interest in pharmacological preparations. The present investigation was aimed to study Nigella sativa seeds and Cassia angustifolia leaf solvent extract (petroleum ether, methanol and aqueous solutions) for phytochemical screening, GC-MS characterization, Antimicrobial and Antioxidant assay. Qualitative phytochemicals in both Nigella sativa seeds and Cassia angustifolia leaf contains high quantity (+++) of alkaloids, Carboxylic acid, Coumarins, Phenol, Resin, Saponin, and Steroid. Antimicrobial activity was determined with two gram positive (Bacillus subtilis and staphylococcus aureus) bacteria. Methanol extract of Nigella sativa seeds shown highest zone of inhibition (19.66 ± 9.29 mm) against staphylococcus aureus, Escherichia coli and klebsiella pneumonia. Petroleum ether extracts of both Nigella sativa seeds and Cassia angustifolia leaf extracts shown highest zone of inhibition against klebsiella pneumonia (20.00 ± 5.03mm and 21.66 ± 4.16 mm, respectively). From the analysis of antioxidant potential of Nigella sativa and Cassia angustifolia extracts through DPPH free radical scavenging at three different concentration (5,...
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Posted by admin on Jan 31, 2018 in |
Eleven new compounds belonging to series 7-[5-(substituted) phenyl – 4, 5-dihydro-1H-pyrazol-3-yl]-2,3-diphenyl-5H, 10H – benzo[g] quinoxaline – 5, 10 – dione (Compound 7a-k) were synthesized by multistep synthetic scheme. The newly synthesized compounds were screened for their in-vitro antimycobacterial activity against Mycobacterium tuberculosis H37Rv by L.J. Slope (Conventional) Method. Compound 7j having 4-CH3 at phenyl ring attached to 5-position of pyrazoline ring and compound 7i having 3,4-(OCH3)2 group on the above mentioned position have been observed as most and least active antimycobacterial compounds...
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Posted by admin on Jan 31, 2018 in |
Controlled release systems for protein and peptide vaccines based polymers have been considered for many applications in medical therapies. Chitosan is non-toxic and easily absorbed biopolymers and is often used in many researches to sustained release drugs and proteic vaccines. This study investigates the kinetics of release in vitro of bovine serum albumin (BSA) from chitosan microparticles and the effect of the preparation process of the microparticles in the release rate. Chitosan microparticles were design by a coacervation / precipitation method under different time’s sonication and different protein / chitosan ratio were used (0, 5 to 2 g. g-1). To the in vitro study of the protein release, the microparticles were resuspended in PBS pH 7.4 at 37 °C under stirring and the samples in different intervals were aliquoted to dosage of BSA release. The morphology of the chitosan microparticles was examined with Electron Microscopy, which showed particle size between 10 – 30 µm and porous structures. Studies were conducted to investigate the BSA stability during the process of...
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Posted by admin on Jan 31, 2018 in |
The concentration levels and human health risk of iron, cadmium, zinc, nickel, copper and lead were determined in 25 popularly consumed types of commercially available herbal drugs in Southern Nigeria. In each type, three brands were selected and analysed. The study area was divided into five sampling zones (A – E) and the herbal drugs were categorized into five clusters based on their advertised usage. The samples were digested using HNO3: HClO4 mixture in the ratio of (4:1) and metals analysis were carried out using Atomic Absorption spectrophotometer. The results showed the concentration of the metals in the ranges: Fe = 4.524 ± 0.011 to 70. 121 ± 0.002 µg/g; Cd = 0.012 ± to 0.620 ± 0.002 µg/g; Zn = 0.03 ± 0.01 to 42.010 ± 3.40 µg/g; Cu = 0.160 ± 0.000 to 66.21 ± 2.11 µg/g and Pb = BDL to 22.011 ± 0.850 µg/g. The provisional weekly intake (PWI) of the metals did not exceed the recommended provisional weekly tolerable intake (PTWI) except nickel in...
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Posted by admin on Jan 31, 2018 in |
Bisphenol A (BPA) is primarily used in the manufacture of plastics and resins. There are many scientific reports that point to the adverse effects of this chemical to different animal models. There has been an increase in use of plastic during the last couple of decades and this has increased human exposure to plasticizers like Bisphenol A. But since little is known on how these endocrine disruptors especially BPA promote cancer progression, the study investigated the effect of BPA on hepatocellular carcinoma cell line Hep-G2 by evaluating the expression of molecules involved in the processes of inflammation, cancer and angiogenesis. Cell viability studies using MTT assay, RT-PCR studies and Western blotting studies were performed to check effect of Bisphenol A on liver cancer cells. Cell viability studies, RT-PCR studies and Western blotting studies showed a dose dependent increase in the expression of factors involved in cancer progression which decreased when the cells were treated with Meloxicam, a COX-2 inhibitor. The results indicate that BPA up-regulates the expression of factors...
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