Posted by admin on Feb 28, 2018 in |
Antibiotics are most important commercially used secondary metabolites, produced by many soil microorganisms i.e., bacteria and fungi and employed in wide range. Most important antibiotics used today are of microbial origin. The emergence of the antibiotic resistance and need of broad spectrum antibiotics is in focus and in demand. In present study, soil samples from different areas were collected i.e., the sampling is classified based on its micro and macro environment (waste polluted soil) (normal street soil) and (agricultural soil), from a local soil and analyzed for the antibiotic production. After primary screening, bacterial isolates were identified as Micrococcus roseus, Brevibacterium sp., Bacillus subtilis, Bacillus anthracis and Bacillus cerus, through biochemical characterization, and fungal isolates were identified as Tricho-cladium opacum, Rhizocotania sp., Epicoccum nipponicum, Aspergillus niger and Cladosporium cladosporides through microscopic and macroscopic identification and checked for antibiotic activity against some common gram positive and negative bacteria namely, Staphylococcus aureus and Escherichia coli. The antibiotic test indicates that Bacillus subtilis, Bacillus anthracis, Epicoccum nipponicum, Aspergillus niger and Cladosporium cladosporides...
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Posted by admin on Feb 28, 2018 in |
Ganoderma is a miracle edible mushroom with unique composition and miraculous health benefits. The investigation aimed to elucidate interactions for the active compounds of Ganoderma lucidum namely Ganoderol A, Ganoderol B, Ganoderal A and Ganoderic Acid Y with Lanosterol 14 alpha demethylase enzyme using molecular docking software Arguslab. The effect of ganomycin1 and 2 as an inhibitor against HIV 1 protease and Tyrosinase were carried out using molecular docking software DOCK 6.3. The in-silico docking analysis of Ganoderma lucidum triterpenes was found to be effective as inhibitors of Lanosterol 14 alpha demethylase. The grid score and best scored conformations of Ganomycin 1 and 2 suggest that both have a significant inhibitory effect on these enzymes, comparable to the known inhibitors. The docking results showed significant inhibitory action of these compounds indicating the possibility that they can be used as potent drugs in the future. Thus these natural compounds have diverse structural and biological properties which can serve them to be utilized as a valuable lead molecule for the treatment...
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Posted by admin on Feb 28, 2018 in |
Lipid metabolism in animals has recently gained growing interest among researchers. Among organic compounds, it is only protein and nucleic acids that for a long time have remained in primary focus of research, though lipids are now known to be essential for the origin of life and development of organic forms. Metabolic processes are very active in the bodies of high-producing cows. Large amounts of energy and nutrients are utilized to synthesize milk; all body systems function intensely. Consequently, there are increased demands for adequate supply of energy and all nutrients including lipids. Insufficient or excessive intake of the latter leads to disruption of metabolism, lower productivity, and deterioration of animal health. At present, there is some controversy in the Russian and foreign literature in regard to on the optimal fat content in the dry matter of the rations of lactating cows, especially high-producing ones. Researchers recommend rather wide ranges of fat concentrations in the diets. The issue that remains insufficiently explored is the effect of various fat levels...
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Posted by admin on Feb 28, 2018 in |
The objective of the present study is to carry out the photochemical investigation and hepatoprotective activity study of different extracts of Scoparia dulcis. Scoparia dulcis Linn. belongs to the family Scrophulariaceae and have ruminate medicinal properties. The different extracts of this plant were prepared by successive extraction with petroleum ether, chloroform and ethanol. These extracts (PEESD, CESD and EESD) were then taken for preliminary phytochemical screening using standard methods. The hepatoprotective activity of the different extracts of Scoparia dulcis was evaluated by using carbon tetrachloride induced liver damage in experimental rats. In hepatotoxicity induced animals, an oral dose of 300 mg/kg, of the petroleum ether, ethanol and chloroform extracts of Scoparia dulcis exhibited a compelling decrease in marker enzyme levels and increased levels of antioxidant enzymes. In the above dose the plant extracts produced a significant decrease in the Lipid peroxidase levels in the tested animals against Carbon tetrachloride induced liver toxicity. The chloroform extract was found rich in phytochemical constituents and had the highest hepatoprotective activity. From the...
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Posted by admin on Feb 22, 2018 in |
The current treatment of inflammation includes NSAIDs, selective COX-2 inhibitors, glucocorticoids and anti-TNFα has peptic ulcer, renal and hematological toxic side effects as well as some of these drugs are more expensive. Hence it is necessary to evaluate the new cost effective as well as less toxic drug. Aloe vera showing a lots of promise and hence researcher all over are trying to harness the anti-inflammatory potential of Aloe vera. Objective: To study the anti-inflammatory and immunomodulatory activity of Aloe vera gel extract. Methodology: The anti-inflammatory activity was evaluated by using carrageenan induced rat paw edema model for acute inflammation and cotton pellet granuloma for chronic inflammation and to evaluate the ulcer potential. The immunomodulatory activity was evaluated by phagocytic activity of Polymorphonuclear cells (PMNs) on Candida cells. Results: The Aloe vera gel extract in the doses of 100mg/kg, 200mg/kg, show significant anti-inflammatory activity while 400mg/kg show equal anti-inflammatory activity as that of Aspirin on acute model of inflammation. Aloe vera gel is not having ulcer potential as that...
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