Posted by admin on Jan 31, 2018 in |
Mesua ferrea Linn. (M. ferrea) is an endemic medicinal plant used for the treatment of inflammation, rheumatism, bronchitis, fever, headache, asthma and skin diseases. The aim of this study was to investigate the anti-inflammatory activities of 80% ethanol extract from stem bark of Mesua ferrea Linn. using animal models. Female Wistar albino rats weighing (150-200g) were used for the study. Anti-inflammatory activity test was done on rats at two different doses (100 and 200mg/kg) by using carrageenan induced paw edema, cotton pellet induced granuloma and formalin induced paw edema. The results showed that the 80% ethanol extract of stem bark (SBE) 200mg/kg and indomethacin (10mg/kg) exhibited significant reduction of edema in carrageenan induced rat paw edema 39.3 (p < 0.05) and 38.8% (p < 0.01) respectively at the end of 4 h. In cotton pellet granuloma test, SBE (200mg/kg) and indomethacin significantly reduced the wet (42.5 and 40.6% (p < 0.01)) and dry (46.7 and 42.8% (p < 0.01)) weight of the cotton pellet respectively, while in formalin induced...
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Posted by admin on Jan 31, 2018 in |
A simple stability indicating reversed-phase HPLC method was developed, validated and subsequently alkaline degradation kinetics are also determined for the estimation of Flavoxate Hydrocloride (FVH) present in pharmaceutical dosage forms. The proposed RP-HPLC method utilizes a LiChroCART – Lichrosphere 100, C18 RP column Hibar® (250 × 4 mm, 5 μm) in an isocratic separation mode with mobile phase consisting of methanol and water in the proportion of 50:50% (v/v), at a flow rate of 0.8 ml / min and the effluent was monitored at 315 nm. The retention time of FVH was found to be 2.92 min. Stability of FVH was investigated as per ICH – prescribed stress conditions including acidic, alkaline, thermal, oxidative and photolytic conditions. Significant degradation of FVH was observed under all studied stress conditions. A kinetic study was conducted to investigate the alkaline degradation of FVH at different temperatures; reaction rate constants, half-life times and activation energy were calculated. The described method was linear over a range of 1 – 300 µg/ml. The percentage recovery...
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Posted by admin on Jan 31, 2018 in |
The purpose of present work was to improve the oral bioavailability of embelin by formulating this hydrophobic phytoconstituent as self emulsifying drug delivery system (SEDDS). SEDDS improves the oral bioavailability by overcoming the dissolution step, enzymatic degradation and hepatic first pass metabolism of phytoconstituent. On the basis of solubility study of embelin, ethyl oleate, tween 80 and span 20 were selected as oil, surfactant and co surfactant respectively for the formulation. Phase diagram study revealed the ratio of surfactant and co surfactant (Smix) for effective self emulsification. Prepared batches of formulation were evaluated for various physicochemical parameters which includes thermodynamic stability study, emulsification time, morphology study of droplets, determination of zeta potential, viscosity determination, percentage drug content, long term stability study. Pharmacokinetic study of plain drug, marketed tablet and SEDDS of embelin was also done which includes in vitro drug release study which revealed that the pattern of drug release is almost uniform and constant moreover embelin SEDDS have highest percentage cumulative drug release (93%) as compared to the...
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Posted by admin on Jan 31, 2018 in |
Introduction: To assess the level of knowledge, attitude, and practices of women of reproductive age of 20 – 70 years on their understanding of the risk factors of cervical cancer, its early detection, and prevention. Methods: A cross-sectional study was conducted in rural areas of Komarapalayam from August 2016 to January 2017 by using pre-tested modified questionnaire. The purpose of the questionnaire was to assess the demographic data, exposure to risk factors, knowledge, attitude, and practice of the participants toward cervical cancer. Results: A total of 500 married women have participated in this study. Majority 26.6% had the secondary education; most common 34% age group being 31 – 40 years. 39% had first childbirth and 34.6% were married between 19 to 22 years; 27.8% had abnormal uterine bleeding were the most common prevalence of risk factors. Mostly 98.4% and 96.6% were heard about cervical cancer and its screening. Many women 82.2% had never undergone cervical cancer screening and 98.9% of the women had never been vaccinated. The main sources...
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Posted by admin on Jan 31, 2018 in |
A novel Mannich base ligand 1-(2,5-dioxopyrrolidin-1-yl) (4-hydroxy phenyl)methyl)thiourea (PSTU) and its Manganese (II) and Cobalt (II) complexes have been synthesized and characterized by elemental analysis, molar conductance measurements, magnetic susceptibility measurements, FT-IR, 1H NMR and 13C NMR spectroscopic studies. The antimicrobial activities and antioxidant activities of the free ligand and its metal complexes were also performed. The results revealed enhanced antimicrobial activities against Bacillus subtilis (gram+ive) and Escherichia coli (gram–ive bacteria) and also significant antioxidant activity. In addition, DNA binding properties of the ligand and its metal complexes have been investigated by UV-Visible absorption spectroscopy, viscosity measurements, emission spectra and gel electrophoresis, which suggest that the metal complexes act as efficient DNA binders. The results shown that the nuclease activity Co(II) complex was effective which could induce scission of pBr322 super coiled DNA effectively to linear form in presence of H2O2 as oxidizing...
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