Posted by admin on Feb 28, 2018 in |
The aim of the present study is to evaluate the antifungal effect of Anvillea radiata and to test the storage efficiency of soft wheat by coating. Phytochemical screening conducted on the Anvillea radiata extracts revealed the presence of the elements has known medical and physiological activities. Our results show a correlation between the plant part, extract concentrations and mold species. In general, all the sensitivity curves show an increasing trend with more or less steep slopes depending on the mold strains and concentration. This action makes it possible to use the method of storage soft wheat by coating grains for increases the storage time and reduces the risk of alteration by...
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Posted by admin on Feb 28, 2018 in |
Thymoquinone (TQ) is the major active component of black seed essential oil which possess encouraging effects against cancer cells. The antitumor activities of TQ were verified in breast carcinoma in-vitro and in-vivo. Most of the times, the cellular responses to apoptosis, after TQ treatment, are evaluate by flow cytometry using annexin V-propidium iodide (PI) staining method. Although flow cytometry has become an important tool to study characteristics of individual cells but induction of auto fluorescence (AF) can decreases the technique sensitivity. In order to investigate the effect of TQ on AF induction, we assessed the fluorescence after TQ treatment. We present evidences suggesting that the change in AF is depend on cell type and TQ concentration. We show here, MDA-MB231 breast cancer cell line treated with different concentration of TQ exhibits elevated level of auto fluorescence (AF) when analyzed by flow cytometry. Consistent with this view, MCF-7 cells did not show any AF upon TQ treatment, in contrast to MDA-MB231. Finally, we presents data demonstrating that changes in AF...
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Posted by admin on Feb 28, 2018 in |
Aim / Background: Aim of this study is to provide scientific evidences that supports the traditional use of Begonia picta; helps to prepare Begonia picta Ayurvedic and / or allopathic formulation for immediate use of herb. Methods: In present study biological screenings were done by following Cytotoxicity pre-screening by Brine shrimp lethality bioassay, anti-hyperglycemic activity evaluation by oral glucose tolerance test and anti-inflammatory effect evaluation by hind paw edema method. Results: The brine shrimp lethality bioassay showed that the hexane and methanolic extracts of Begonia picta was mildly toxic to Artemia salina naupalii having LC50 values 162.0690 µg/ml and 237.263 µg/ml respectively. The highest percentage of blood glucose lowering effect was found in 180 minutes for standard drug (32.52%), Begonia picta extract 200 mg/kg (39.62%), and 400 mg/kg (43.17%). Anti-inflammatory activity test result showed that maximum inhibition activity observed during 5 hour: 31.74% of standard, 28.08% of test (200 mg/kg) and 29.78% of test (400 mg/kg). Conclusion: The brine shrimp lethality bioassay confirmed that the hexane and methanolic extracts...
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Posted by admin on Feb 28, 2018 in |
Chlorpheniramine maleate and Dextromethorphan are used in treatment of cough caused by allergies and the common cold by decreasing intra ocular pressure. The present study was designed to develop and validate a simple, sensitive, precise and accurate reverse phase high performance liquid chromatographic (HPLC) method for simultaneous determination of Chlorpheniramine maleate and Dextromethorphan in tablet dosage form. Chromatographic separation of these two drugs was achieved on Discovery C18 column (250 x 4.6 mm, 5 μm) as stationary phase with a mobile phase of Water (pH 2 adjusted with OPA): Acetonitrile (60:40v/v) at a flow rate of 1 ml/min and PDA detection at 218 nm. The retention times of Chlorpheniramine maleate and Dextromethorphan were found to be 3.0 min and 3.6 min respectively. The proposed method was validated for system suitability, linearity, accuracy, precision, LOD, LOQ and robustness. The calibration curves were linear in the concentration range of 50% to 150% of the working concentration (r2 = 0.999) for both the drugs in binary mixture. The LOD was found to...
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Posted by admin on Feb 28, 2018 in |
The objective of this study was to evaluate and optimize Eudragit concentration and coating level on metronidazole release from granules for colon delivery using 32 full factorial design. Independent variables selected were Eudragit conc. (10, 15 and 20%) and coating level (10, 15 and 20%), the evaluated responses were percent drug release at 5 h, 24 h and time to release 50% of the drug. The dissolution was carried out at different pH media of 1.2, 7.4 and 6.8. The dissolution data revealed that polymer conc. and coating level were having significant role in achieving optimum formulation. Using surface response, contour plot and statistical equations, the optimum formulation consisting of 15% of polymer conc. and 20% coating level was predicted. Experimental data showed that the granular formulations releases no drug at pH 1.2 (simulating stomach) and only 12.85 ± 1.63% at pH 6.8 (simulating small intestine) and controlled at pH 7.4. The result of this study showed that the factorial design is a suitable tool for optimization of coating...
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