Posted by admin on Oct 31, 2017 in |
Multiple causes of liver diseases that will affect the health related quality of life and are dispersed globally. It is difficult to diagnose the different stages of liver disease. Scores like Child Pugh Score(CPS), Model For End Stage Liver Disease(MELD), Monoethylglycinexylidine(MEGX), Von willibrand factor (vWF) etc are used to detect the progression of liver disease like hepatitis, cirrhosis, fibrosis etc. Child Pugh Score is important and traditional component for evaluation of liver disease. It is easy to perform at bed site. MELD is for predicting short term mortality and is more objective but not superior to CPS due to some reasons. These scores contain subjective as well as objective parameters for better prediction of liver related disease. MEGX and von villibrand factor are better simplest markers for the prognosis of liver disease. MEGX is competent to various scores and useful marker for impaired metabolic activity and blood flow in cirrhotic patient. vWF is new non invasive method for survival rate of patient with compensated and decompensated liver disease. By...
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Posted by admin on Oct 31, 2017 in |
Hepatic cancer is one of the most causes for cancer death in the world. The aim of this work was to compare and survey the cytotoxic activity of a group of differently classed flavonoids and three plant extracts known for their high antioxidant activities against Hep-G2 human cancer cell line. Also to isolate some flavonoids from the plant extracts using easier, modified methods of higher yield and lower time of extraction than conventional ones. Different flavonoidal classes were tested against Hep-G2 human cancer cell line using SBR assay. Doxorubicin was used as a positive control. Dihydroquercetin was found to be more potent even than Doxorubicin. It showed the higher activity with IC50 = 4.43±0.08 μg/ ml followed by quercetin and rutin with IC50 = 7.88±0.89 and 11.2 ±0.97μg/ ml respectively. Among the tested extracts, rosemary alcoholic extract was the potent followed by green tea alcoholic extract and orange peel alcoholic extract was the less potent. Their IC50 values ranged from 11.5±1.02μg/ml to 48.2 ±1.8μg/ ml. Diosmin and catechins showed...
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Posted by admin on Oct 31, 2017 in |
Over time, ethnomedicinal plants have been praised hugely for the presence of diverse pharmacologically active phytochemicals and have been used traditionally in primary medication to cure various human diseases or disorders. In the present study, different phytochemical constituents were analysed and free radical scavenging activities of Dregea volubilis stem bark (DvSB) extracts were assayed. Different in vitro scavenging tests such as DPPH· scavenging assay, O2·– scavenging assay, OH· scavenging assay and H2O2 scavenging assay were employed to estimate the free radical scavenging potentiality. Results revealed that methanolic extract had superior scavenging activity over the hot water extract. Qualitative analysis of phytochemicals showed the presence of reducing sugars, phenolic compounds, flavonoids, resins, glycosides, cardiac glycosides and steroids; and quantitative phytochemical analysis for polyphenols, flavonoids, flavonols, proanthocyanidins and alkaloids were made by standard procedures. Hence the study reveals that DvSB has significant scavenging properties, thus validating the ethnic usage for treating stress-related complications and restoration of good...
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Posted by admin on Oct 31, 2017 in |
The objective of the present study is to identification of the oxidative degradation impurity in the Granisetron hydrochloride active ingredient and also the novel method developed based on the forced degradation studies performed on Granisetron Hydrochloride to well resolve the impurities from the API. Forced degradation studies were conducted as per ICH technical requirements prescribed stress conditions using acid, base, oxidative, thermal stress, and photolytic degradation to show the stability indicating power of the method. Significant degradation was observed during basic hydrolysis and in oxidative studies and no degradation was observed in other stress conditions. Characterization of oxidative degradation impurity was conceded with a combination of analytical and preparative LC isolation followed by LC-MS/MS, IR and 1H and 13C NMR and 2D NMR spectral data. The HPLC chromatographic conditions were chosen to achieve satisfactory resolution between the impurities and API with shortest analysis time. Granisetron hydrochloride and its impurities were separated on Xbridge phenyl column (150mm×4.6 mm, 3.5 microns particle size). The mobile phase A composed of 10mM of...
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Posted by admin on Oct 31, 2017 in |
Background: Lenalidomide plus Dexamethasone (Len-Dex) and VAD (Vincristine, Doxorubicin and Dexamethasone) regimen are the two common drug therapies involved in the treatment of Multiple myeloma. These two groups of drugs act by different mechanisms and their safety profile also varies. Objectives: To compare the safety of Len-Dex versus VAD regimen based on World Health Organization toxicity criteria by grade as well as the performance status of the patients of both the regimen by using Karnofsky performance status scale definitions rating. Materials and Methods: Eighty patients (forty in each arm) of newly diagnosed cases of multiple myeloma, who were willing to give the informed consent, were included in the study. Their baseline investigations and follow up investigations were collected at regular intervals, based on these values, the adverse effect profile as well as the performance status were evaluated and the results were compared and analyzed. Results: In Len-Dex regimen, constipation, leucopenia, thrombocytopenia, slow wound healing, sedation, renal toxicity and hepatotoxicity were high. VAD regimen produce higher incidence of nausea, vomiting,...
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