Posted by admin on Oct 31, 2017 in |
The present work deals with formulation of topical gels containing aqueous extract of P. acerifolium seeds and also evaluation of prepared gels against some Gram-positive and Gram-negative bacteria. The antibacterial activity of various P. acerifolium seed extracts was measured through determining minimum inhibitory concentration (MIC) by cube dilution method and zone of inhibition by disc diffusion method against E. coli, P. aeruginosa and S. aureus. Aqueous P. acerifolium seed extracts exhibited comparatively higher antibacterial potential in comparison with that of other extracts and employed to formulate topical herbal gels using 4.5% sodium carboxymethyl cellulose as gel base. Prepared herbal gels were tested for pH, viscosity, extrudability and spreadability. All the prepared gels were subjected to antibacterial study by determining zone of inhibition by disc diffusion method. Herbal gels containing 15% aqueous P. acerifolium seed extract exhibited better zones of inhibition against E. coli, P. aeruginosa and S. aureus in comparison with that of 7.5% and 10%. From the results of this study, it can be concluded that herbal gels...
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Posted by admin on Oct 31, 2017 in |
Betel leaves have been eaten traditionally as a quid in many South East Asian countries. Its taste and aroma is because of the presence of essential oils, namely eugenol. Eugenol is an important phytochemical with analgesic, anti inflammatory and anaesthetic properties. Eugenol further finds applications in cosmetics and food industry. Betel leaves being perishable, become a major concern for agro waste. Yellow dry leaves and the petioles also go waste. In this paper, HPTLC fingerprint and eugenol content were studied in three varieties of fresh betel leaves as also in yellow leaves and in their petioles. Seasonal variation in eugenol content was also studied. The fingerprint of the leaves showed 10 bands with one of the marker peaks at 0.35 which corresponded to eugenol. It was found that Benarasi betel leaf has maximum eugenol content (average 1.1104%). Nagarvelli and Kolkata leaves have comparable eugenol content (0.659% and 0.637% respectively). In all the three leaf varieties, eugenol content was found to be the highest in winter season, the least in...
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Posted by admin on Oct 31, 2017 in |
A thermostable bacterial 3-cyanopyridinase enzyme has been identified that catalyze 3-cyanopyridine to nicotinic acid and ammonia. Nicotinic acid is mainly used in food, pharmaceutical and biochemical industries. This study was undertaken to optimize the purification of 3-cyanopyridinase enzyme and characterization of its various kinetic properties. The partial purification of enzyme has been carried out in five steps that results in approximately 2.3 fold increased purification with 46.5 % of enzyme recovery. The partially purified enzyme was observed approximately 43 kDa of maximum activity at 50 °C temperature and pH 7.0. The partially purified enzyme showed the Km of 0.91 mM and maximum velocity (Vmax) of 0.59 U. The half-life (t1/2) of partially purified enzyme was about 60 min at optimal conditions. The enzyme has broad substrate specificity, hydrolyzing toxic compounds such as benzonitrile and propionitrile. Thus this novel enzyme has potential in bioremediation process....
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Posted by admin on Oct 31, 2017 in |
To investigate the content of polar auxin transport inhibitor flavonoid quercetin (Q) and topo-isomerase I-DNA inhibitor alkaloid camptothecin (CPT) from Gracilaria corticata (GC), red seaweed from Surathkal beach, Karnataka a comparative study was carried out. Methods: The content of Q and CPT was quantified in the ethanolic extract of GC using thin layer chromatography (TLC) and high performance liquid chromatography (HPLC). The HPLC analysis resulted in a sharp peak of authentic CPT and Q at the retention time of 2.445 min and 3.540 min respectively. The peaks of ethanolic extract of GC spiked with CPT was found at the retention time of 1.630 min, 1.690 min and 2.000 min and retention time in the ethanolic extract of GC spiked with Q was found to be 1.600 min and 1.713 min. The method developed for this study shall stand appropriate for the quantification of secondary metabolites and screening of flavonoid and alkaloid contents in the...
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Posted by admin on Oct 31, 2017 in |
In present study antiproliferative activity of Rutin was evaluated on HeLa cell line induced cervical cancer in rats. For this study, 30 rats were divided into 5 groups and each group containing 6 rats each. Group I- normal saline treatment for 45 days, Group II- cancer cells (1×106 cells in 0.1ml/rat), Group III– 5-Fluorouracil (20mg/kg + 1×106 cells in 0.1ml/rat), Group IV- Rutin (50mg/kg + 1×106 cells in 0.1ml/rat), Group V- Rutin (70mg/kg + 1×106 cells in 0.1ml/rat). After 24 h of tumour inoculation intraperitoneally, Rutin was administered daily for 45 days. After administration of last dose followed by 18 hrs fasting, rats were sacrificed for observation of antiproliferative activity. The change in body weight, body circumference of tumour bearing hosts and simultaneous alterations in haematological profile, serum (Triglycerides, Total protein, Total cholesterol, GGT, ALP and glucose) and liver biochemical parameters (lipid peroxidation, GSH and antioxidant enzymes-CAT, GPx) were estimated. The changes in tissue enzymes-Glucose-6 phosphate dehydrogenase, Hexokinase, Succinate dehydrogenase and CytochromeP450 levels were also estimated. Rutin maintained the...
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