Posted by admin on Nov 30, 2017 in |
Objectives: The aim of this study was to assess the drug utilization pattern and teratogenicity risk among pregnant women attending a tertiary care hospital, Andhra Pradesh. Materials and Methods: A cross-sectional observational study was carried out on antenatal women (total=1163) more than 18 years of age attending the antenatal clinic of Obstetrics and Gynaecology (OBG) Department. Demographic and pregnancy-related details were collected in case-record forms. Each prescription prescribed was assessed as per USFDA (United States Food and Drug Administration) categorization of drugs for use in pregnancy and WHO (World Health Organisation) core prescribing indicators. The resultant data was analyzed using MS Excel 2007 and the Statistical Package for Social Sciences (SPSS) statistical software, version 21. Qualitative variables were expressed as percentages and Inter-group comparison was done using Chi-square test. For all statistical analysis, p<0.05 was considered statistically significant. Results: Of the total number of drugs used, Category A drugs were the most common (75.5 %) followed by Category B (17.5 %), Category C (5.28 %), Category D (1.55 %)...
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Posted by admin on Nov 30, 2017 in |
Biochemical characterization of enzyme is the most fundamental aspects of enzymology from industrial point, as it is desirable to choose an enzyme which will have the fastest reaction rate per unit amount of enzyme which indicates the maximum effect for the minimum amount of added catalyst. In this study, Protease enzyme was extracted from Z. officinale and purified by using various down streaming techniques up to apparent homogeneity from which a model has been generated based on mathematical modelling. Neural networking approach has been applied to optimize the incubation time, pH stability, temperature stability, initial substrate concentration and initial enzyme concentration on casein hydrolysis. The purified protease from Z. officinale is thermo stable and appears to be a promising candidate in applications on various industrial...
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Posted by admin on Nov 30, 2017 in |
Aeromonas hydrophila is a gram negative bacterium which is primary or secondary cause of ulcers and hemorrhagic septicaemia in fish. The treatments for this infection are only restricted to some antibiotics. So, novel materials are being searched for combating with bacterial infections and the resulting consequences. In this presence study, the green synthesized silver nanoparticles using Hibiscus rosa sinensis extract act as reducing agent. The silver nanoparticles have shown bactericidal effects against A. hydrophila isolated from infected fish Catla catla. The silver nanoparticles were characterized by using UV–Visible, fourier transform infra-red spectroscopy (FT-IR) and scanning electron microscopy (SEM) techniques. SEM results showed that the particles were in the size range of 5µm. FT-IR prediction indicated the presence of C–H stretch, C–H bend, N–H bend, –C≡C– stretch, H–C=O: C–H stretch, C–H stretch respectively on the AgNPs. The maximum zone of inhibition 10 mm observed by normal cured against A. hydrophila is. The synthesized silver nanoparticles exhibited maximum zone of inhibition 11 mm against A. hydrophila. As a conclusion of the...
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Posted by admin on Nov 30, 2017 in |
Globally at present, there are an estimated 366 million people affected with diabetes mellitus. The Indian figure is around 61.3 million diabetics, which is projected to cross 100 million by the year 2030. Owing to rise of diabetes mellitus cases, the economic burden due to diabetes in India ranks the highest in the world. With the increasing prevalence of diabetes, it has resulted in an increase in its complications which has resulted in increasing morbidity, mortality and health expenditure due to the requirement of specialized care. Diabetes Mellitus is one of the most prevalent chronic disorders in the modern world. CRP is a plasma protein which is not normally present in the body but its advent is marked by its elevated levels in the blood during certain specific condition, especially marked during the inflammatory events occurred as a result of any kind of injury. Due to its involvement in inflammation, which is also seen as one of the mediators of progression of type II diabetes mellitus and its various...
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Posted by admin on Nov 30, 2017 in |
The present study aims to synthesize highly stable and dispersed cytotoxic silver nanoparticles (AgNPs) from Tabernaemontana divaricata (T. divaricata) leaves extract, by using noble green approach at room temperature. Phytocomponents were investigated using general methods and found good agreement with reported literature. Green synthesized AgNPs were characterized using UV-Vis, FT-IR, XRD, TGA, FEG-SEM, EDX, TEM, SAED and AFM spectroscopy techniques. XRD and SEAD confirmed polycrystalline nature. TEM study shows spherical morphology with size range 6-20 nm. Suforhodamine B (SRB) assay revealed potent cytotoxicity of AgNPs on A549 cells with IC50 value 32.3µg/ml. As per SRB assay in case of AgNPs, GI50 = 37.1µg/ml, TGI = 68.9µg/ml, LC50 = > 80µg/ml, while T. divaricata exhibits GI50, TGI, LC50 values = > 80µg/ml, indicating greater biocompatibility of AgNPs in comparison to T. divaricata leaves extract. This investigation reveals that T. divaricata mediated AgNPs has a great potential for the development of an efficient anticancer nano drug with minimal side effects to combat against life-threatening lung...
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