Posted by admin on Nov 30, 2017 in |
In recent years, in silico approaches have been predicting novel drug targets. The objective of this study was to analyze the inhibitory action of canthin-6-one alkaloid by computational docking studies. For this study, natural metabolite canthin-6-one isolated from Simarouba glauca was used as ligand for molecular interaction. The crystallographic structure of molecular target Epidermal Growth Factor Receptor (EGFR) tyrosine kinase was obtained from Protein Data Bank (PDB) database (PDB ID: 1M17). Gemcitabine, Cisplatin and Thiotepa were taken as the standard for comparative analysis. Computational docking analysis was performed by using online program PATCHDOCK. The canthin-6-one showed optimum binding affinity with a molecular target (EGFR – tyrosine kinase) with the binding energy of (-248.25) as compared to the standard drugs Gemcitabine (-188.48), Cisplatin (-45.26) and Thiotepa (-190.89). These results indicated that canthin-6-one could be one of the potential ligand to treat cancer. These potential drug candidates can further be validated in wet lab studies for its proper...
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Posted by admin on Nov 30, 2017 in |
Background: A cross sectional study, to determine the endemicity, by systematic investigations in institutional settings to assess the prevalence of diseases. Methods: The study was conducted by observational method over a period of seven months regarding the prevalence of particular disease and disease with co-morbidities. Among the several diseases of distinct clinical spectrum, various risk factors may presumably be contributed. This is an epidemiological study conducted prospectively at tertiary care teaching hospital which is a 350 bedded super speciality hospital. This study was conducted for a period of seven months from August 2016 – February 2017 in order to evaluate the cases for endemicity11. Patients’ demographic and diagnostic data between 20 to 80 years of age were collected from the patient’s case survey. Objectives: The main objectives of the our study was to provide awareness about diseased non communicable endemic prevalence of conditions like Diabetes, Hypertension and habitats like alcohol consumption and smoking. The intention of this manuscript would help the clinician to intervening patient’s relevant clinical and therapeutic...
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Posted by admin on Nov 30, 2017 in |
The studies in the present article aims at developing an optimal oral polyherbal nanoemulsions from Carissa carandas (CC) and Carissa spinarum (CS) which are noticed in ethnobotanical herbarium for their pharmacological relevance. Nanoemulsions were developed in different proportions of oil and surfactant systems in order to enhance its oral bioavailability. Solubility studies were carried out for the preparation of the formulation. Pseudoternary phase diagrams were constructed by aqueous titration technique and different polyherbal nanoemulsions were prepared. Formulations selected from o/w nanoemulsion region were subjected to various thermodynamic stability tests. Optimized formulations were evaluated for physicochemical tests. In vitro anti‐inflammatory activity was carried out by inhibiting heat induced albumin denaturation, proteinase activity, red blood cells membrane stabilization and broth dilution assay. Phase diagram with 1:2 S/Smixhas shown largest area of nanoemulsion. In vitro dissolution studies revealed that release of drug from nanoemulsion 2 (96.88±1.29%) was faster than the nanoemulsion1 (85.36±2.12%) in 120 min. The formulation showed satisfactory results for in vitro anti‐inflammatory activity by inhibiting the heat induced albumin denaturation...
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Posted by admin on Nov 30, 2017 in |
There are some herbal contraceptives that have the property to avoid the embryo implantation. Trigonella foenum-graecum Linn. is used in folk medicine as alcoholic and aqueous preparations for prevention of pregnancy. Pregnane- progesterone is one of the important steroid has direct connection with contraceptive activity. Pregnane is a C21 steroid and indirectly, a parent of progesterone. The present work was carried out to find out the responsible phytochemical for contraceptive activity. The objective of this study was to provide an alternative contraception and thus evaluate the pregnane progesterone. The present work was carried out in Trigonella foenum-graecum Linn. with the presence of compound “Pregnane” and its importance in contraceptive activity. The present study showed the presence of [5 Beta]-Pregnane-3,20,beta-diol, [4 alpha] 8- alpha- [4-methyl-3-oxa]oxa-4-ozabutane-1,4 diyl-diacetate with retention time 36.23, peak area 5.335% (C28H43NO6 Mol wt- 489). This pregnane was found first time in this plant. Pregnane progestin contraception is effective and well tolerated, thus providing an excellent contraceptive alternative to the currently used methods. Pregnane can be isolated from Trigonella foenum-graecum Linn. for the...
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Posted by admin on Nov 30, 2017 in |
Quorum-sensing is a phenomenon in which bacteria exhibit several density dependent phenotypes. Quorum-sensing is achieved through production of auto – inducer which on acquiring a threshold concentration, activates the genes, bringing into effect the concerned phenotypes. Ayurvedic medicinal plant extracts are being used to treat a plethora of infectious disease and are known to be rich in phytochemical compounds. This work aims at screening Rosemarinus officinalis and Valeriana officinalis for their anti- quorum-sensing activity using microbial biosensor strains which includes Pseudomonas chlororaphis (aureofaciens) 30-84, S. aureus agr P3::blaZ pRN8826 and C. albicans 10231. Initially, the medicinal plant extracts are made and minimum inhibitory concentration (MIC) of these extracts is determined. Qualitative phytochemical tests are performed with the extracts in order to find out the phytochemical present in these medicinal plant extracts. This is further followed by reporter assays for the detection of anti-quorum sensing activity involving the microbial biosensor strains and then quantification of the activity. The Rosemary leaves and the Valeriana roots containing alkaloid, catecholic tannin and flavonoid...
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