Posted by admin on Nov 30, 2017 in |
Human adipose tissue-derived stem cells (hADSCs) are promising for cellular therapy as they are multipotent, which consistently express high CD90, CD105, CD73 and low hematopoietic surface markers. Flow cytometry is a routine technology that is used in most laboratories and can be used to study hADSC characterization, but the instrument needs strict calibration to assure the quality of the results. However, for rarely used fluorophores, calibration is often neglected. This study aimed to compare calibrated and non-calibrated fluorescence (FL) in flow cytometry performances. hADSCs were isolated from lipoaspirate and cultured in human platelet lysate supplemented medium. Passaged cells were used for characterization. Cells stained with positive (CD90-FITC, CD105-PerCP Cy5.5, CD73-APC) and negative (CD34, CD45, CD11b, CD19, HLA-DR)-PE marker cocktails were subjected to flow cytometry analysis. In the instrument, FITC, PE, PerCP- Cy5.5, and APC were detected by FL1, FL2, FL3, and FL4 respectively. Routine calibration of FL1, FL2, and FL3 was done, except for FL4. Therefore, data from calibrated and non-calibrated FL4 were analyzed. hADSCs strongly and consistently expressed...
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Posted by admin on Nov 30, 2017 in |
Anti-inflammatory activity of the SC-EA extract of the marine Streptomyces carpaticus was studied in Wistar rats using acute and chronic inflammatory models like carrageenan induced paw edema and cotton pellet induced granuloma models respectively. The acute toxicity of SC-EA extract was tested on adult Wistar rats. The maximum dose of SC-EA extract at 2000mg/kg does not show any sign of visual changes in physical behaviour, protein level and enzyme activities of AST, ALT, and ALP after 14 days of administration in rats. The SC-EA extract of 50mg/kg and100 mg/kg, p.o. demonstrated the inhibition of paw edema to 42.18% and 47.08% respectively and standard diclofenac showed 46.78% inhibition after 4 h of carrageenan treatment. In cotton pellet induced granuloma, maximum inhibition was accounted with SC-EA extract 58.3% at a dose of 100mg/kg and the suppression of inflammation was comparable to Diclofenac (30mg/kg), which reduced the weight of cotton pellet granuloma by 56.5%. The hydroxyproline which is a major component of the protein collagen was also estimated in SC-EA extract treated...
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Posted by admin on Nov 30, 2017 in |
Justicia wynaadensis, an endemic medicinal plant found in the regions of Western Ghats is a herb, belonging to the family Acanthaceae. The following study was carried out to evaluate the in vitro cytotoxicity of the leaf extract of Justicia wynaadensis. Four different extracts were prepared by using water and methanol [aqueous fresh leaves extract, methanolic extract of dry leaves powder, hot (boiled) and cold (macerated) aqueous extracts of dry leaves powder]. Preliminary screening of extracts showed the presence of flavonoids, phenols and terpenoids and its quantitative estimation was done using HPLC. Antioxidant activity was evaluated by DPPH and ABTS assay. The cytotoxic effect of all the four extracts were tested by MTT assay on two cancer cell lines MCF7 (Breast cancer cell line) and HCT116 (Colorectal cancer cell line). The cold aqueous extract had the highest cytotoxic effect on both the cell lines at IC50 values of 20.76 µg/ml and 28.46 µg/ml...
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Posted by admin on Nov 30, 2017 in |
Malaria has been a major life threatening mosquito borne disease. Unavailability of any effective vaccine and recent emergence of multi drug resistant strains of malarial pathogen Plasmodium falciparum continues to cause persistent deaths in the tropical and sub-tropical region. As a result, there is a growing demand for new targets for more effective anti-malarial drugs. A novel microneme protein, named sporozoite microneme protein essential for cell traversal (SPECT), is produced by the liver-infective sporozoite of the malarial parasite. In the present study, the 3D conformation of Plasmodium falciparum SPECT (PfSPECT) protein was predicted using homology modelling and refined by 50 nanoseconds of MD simulation. The overall quality of the model was validated using PROCHECK and ERRAT. The PROCHECK results showed 91.4% of backbone angles were in the allowed region and ERRAT score of 85.9% suggested a high quality model. All docking calculations were performed using the “Extra Precision” (XP) mode of Glide docking. A database of 2,76,784 biogenic compounds was used for virtual screening, out of which 164 were identified as hits....
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Posted by admin on Nov 30, 2017 in |
Family Solanaceae is known for its tropane alkaloids which have many medicinal properties. In our study two Cestrum species namely Cestrum diurnum and Cestrum nocturnum which are also ornamental plants are studied as different parts of the plants possess anti-bacterial, anti-malarial, analgesic, anti-inflammatory, inhibitory effect on central nervous system and wound healing properties. The present study was undertaken to analyse the presence of various phytochemicals such as alkaloids, flavonoids, terpenoids, saponins, steroids through preliminary qualitative phytochemical screening of the leaf extract. HPLC(High Performance Liquid Chromatography) was used for the qualitative and quantitative active principle analysis which revealed the presence of nicotine and nornicotine as major alkaloids and comparative study of the two species showed the presence of these alkaloids in large quantity in Cestrum diurnum. The detailed anatomical studies of the part(s) used help in the correct easy identification of the commercial drug in its purest...
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