Posted by admin on Aug 31, 2017 in |
This paper describes a new, simple, precise, and accurate HPTLC method for simultaneous estimation of Oxyclozanide and Levamisole hydrochloride as the bulk drug and in tablet dosage forms. Chromatographic separation of the drugs was performed on aluminium plates pre-coated with silica gel 60 F254 as the stationary phase and the solvent system consisted of Toluene: acetone: ammonia (5:5:0.04 v/v/v). Densitometric evaluation of the separated zones was performed at 225 nm. The two drugs were satisfactorily resolved with R F values 0.28± 0.007 and 0.49 ± 0.009 for Oxyclozanide and Levamisole hydrochloride, respectively. The accuracy and reliability of the method was assessed by evaluation of linearity (400-1200 ng/band for Oxyclozanide) and (200-600 ng/band for Levamisole hydrochloride), precision (intra-day % RSD was 1.20 and inter-day % RSD was 1.51 for Oxyclozanide, and intra-day % RSD was 1.22 and inter-day % RSD was 1.52 for Levamisole hydrochloride), accuracy (0.556 for Oxyclozanide and 1.31 for Levamisole hydrochloride), and specificity in accordance with ICH...
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Posted by admin on Aug 31, 2017 in |
Purpose: To formulate and characterize the bi-layer tablet of doxycycline immediate release and flurbiprofen sustained release. Method: Bi-layer tablets were formulated by wet granulation method. Ethyl cellulose and HPMC K 100 were used as polymer for sustained release layer. Five immediate release formulations were developed with varying excipients. Bi-layer tablets were evaluated by pre formulation and post formulation parameters as stated by USP. Dissolution was conducted in 0.1 N HCl and 6.8 phosphate buffer and resulting data were analyzed statistically by one way ANOVA and drug release kinetics was studied using various pharmacokinetic models. Results: Data from pre formulation confirmed the purity of doxycycline hyclate and flurbiprofen. FTIR confirmed the absence of incompatibility between drugs and excipients. Dissolution profile in 0.1 N HCl and 6.8 phosphate buffer was according to USP guidelines. Regression coefficient (R2) values from kinetic analysis showed that release followed Higuchi model indicating release mechanism followed diffusion transport. Results of one way ANOVA confirmed that there is no statistically significant difference (p > 0.05) between drug...
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Posted by admin on Aug 31, 2017 in |
Antibacterial proteins are evolutionarily ancient, effect or molecules of innate immune defense and are ubiquitously found in all kingdoms. The present study deals with the induction of antibacterial proteins in the haemolymph of Anthereae mylitta drury (Daba TV) by injecting non-pathogenic Pseudomonas DAS-01. Purified protein fraction obtained on ammonium sulphate precipitation followed by ion exchange chromatography had shown antibacterial activity against Pseudomonas aeruginosa AC-3 and Pseudomonas DAS – 01. Flow through obtained in ion exchange chromatography found effective in inhibiting the growth of both P. aeruginosa AC-3 and Pseudomonas DAS – 01. In induced larvae, purified antibacterial peptides obtained by ion exchange chromatography on SDS-PAGE had shown a molecular weight of 16kDa and 24kDa which have antibacterial activity and were not observed in...
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Posted by admin on Aug 31, 2017 in |
Sintering technique, a relatively new, convenient, economic process for making polymeric matrices for controlled release is described here. The objective of the present investigation was to prepare thermally sintered matrix tablets of Atenolol using Eudragit RS 100 as sintering polymer and to study the effect of thermal treatment on drug release; physiochemical properties of the prepared tablets. The tablets are prepared by direct compression method. The compressed fluffy matrices were subjected to thermal treatment at three different constant temperatures like 60 °C, 70°C and 80°C for two different periods like 1.5 hr and 3 hr in hot air oven. The sintering condition markedly affected the drug release characteristics of prepared matrix tablets. Drug release rate was found to be inversely related with the sintering temperature and sintering duration. Hardness of tablets was found to be increased with increasing sintering time and temperature; whereas friability was decreased with sintering. Both percent water uptake and percent erosion of sintered tablets decreased with increase in sintering condition. Based on the evaluation tests...
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Posted by admin on Aug 31, 2017 in |
In recent decades, advances in nanoscience have resulted the rapid development of many novel technological applications for nanoparticles (NPs) in various in Biomedical Sciences. Iron is essential for eukaryotic biochemistry and is an important element in several metabolic processes such as, carrier for oxygen transportation, cell respiration, synthesis of genetic material and protein synthesis. The nematodes Caenorhabditis elegans (C.elegans) represent an excellent organism to investigate the phenotypic effect of different type of nanomaterials on the molecular, cells and entire organism level. C.elegans is widely used in studies of homeostasis, neurolagenerative and metal toxicity. The size of iron oxide and other nanoparticles plays a major role in target cell uptake and elimination from the body. The excess iron damage nucleotides (DNA/RNA) and Protein due to catalyzes the generation of Reactive Oxygen Species (ROS) , while cellular iron deficiency causes cell cycle arrest and cellular death of an organism. Systematic storage and trafficking is required to regulate supply of iron while preventing it from catalyzing unwanted reactions. We have used 36...
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