Posted by admin on Jul 31, 2017 in |
Home based education among clients with diabetes mellitus (DM) by diabetes educator has been cost effective although some educational intervention have strong evidence on cost saving. This study aimed to assess the cost effectiveness of home based education programme on diabetic treatment among clients with diabetes mellitus. Community based randomized controlled trial with experimental design was used in this study. Diabetic subjects who had been undergoing treatment for 3 months in Marai Malai Nagar and 9 villages at Mamandoor were selected by simple random sampling technique. 200 Participants were assigned to the study group, 200 in control group. The study group subjects were engaged in video education program, weekly once 4 sessions and nutritional counseling every 15days 30 mins for 3 months. The overall post interventional result showed from the baseline compared to control group, there was an absolute mean change in direct medical cost (from Rs 2837.05 to 1674.65), cost of investigation (from Rs 319.50 to Rs 261.11), non medical cost (from Rs 369.87 to Rs 110.79), indirect...
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Posted by admin on Jul 31, 2017 in |
The objective of the present investigation is to extract and characterize the active constituent, Gymnemagenin in Gymnema sylvestre leaves using various Chemical and Analytical techniques. Extraction of Gymnema sylvestre leaves powder is done by Continuous soxhlet extraction with 80% ethanol. Identification was done by various chemical tests and TLC. Characterization and quantification by HPLC method, which is quick, reliable and sensitive. LC system Shimadzu with isocratic pumps LC 20-AT, and U.V Detector SPD 20-A was used. Reverse Phase Column C18 (250mm x 4.6mm, 5A° particle size) and a mobile phase consisting of 100 mM ammonium acetate buffer: acetonitrile (75: 25, v/v) were employed. Chemical tests and TLC revealed the presence of Gymnemic acid and its aglycone Gymnemagenin. TLC profile revealed Rf value of 0.41 for Gymnemagenin in the extract and a retention time of 7.366 minutes in HPLC method with a concentration of 3.62 µg/ml in the extract. Various validation Parameters were developed from the HPLC method for identification and quantification of...
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Posted by admin on Jul 31, 2017 in |
The drug Rivaroxaban which was poorly soluble was selected for formulation of SMEDDS due to its poor aqueous solubility and its oral bioavailability which is 66%. Self micro emulsifying drug delivery system was developed to improve its solubility and bioavailability. Solubility of Rivaroxaban is determined in various oils, Surfactants, and co-surfactants by UV-Spectrophotometric method. Rivaroxaban has been shown maximum solubility in oil Peceol oil, surfactant Tween 80 and co surfactant Transcutol HP. A series of Pseudo ternary phase diagrams are constructed to identify micro emulsion region. Various compositions of Oil and Smix are titrated with water to identify micro emulsion region. From pseudo-ternary phase diagrams systems consisting of Peceol oil as oily phase, Tween 80 surfactant, Transcutol HP as co surfactant are selected for formulation. SMEDDS are prepared by selecting oil: Smix ratio 1:9 ….9:1 and Smix ratio 1:3, 1:4. Two mixtures (PTWT-2(1:9), PTWT-2(2:8) are selected for formulation of SMEDDS by keeping amount of drug constant (20mg) in all formulations. Prepared formulations are evaluated for Self emulsification and visual...
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Posted by admin on Jul 31, 2017 in |
A hydrophilic polysaccharide become isolated from green fruit of Musa paradisiaca L. by the usage of wet milling procedure and distilled water extraction the use of sodium sulphite solution, accompanied by way of maceration, centrifugation and sedimentation. The isolated polysaccharides became characterised for physicochemical properties, viscosity and flow behaviour, scanning electron microscopy (SEM), differential scanning calorimetry (DSC) and XRD study. Diverse concentrations of isolated polysaccharide had been studied for binding property towards Maize starch in traditional tablet formulation. In vivo toxicity study detected secure nature of the isolated polysaccharide. Standard, the outcomes of the present study indicated that isolated polysaccharide is resembles to starch and not useful for frozen products. Polysaccharide is shows Pseudoplastic nature with thixotropic behaviour and smooth surface and elongated oval shape granules seen on SEM Micrograph. Form experiment the optimized concentration banana starch as a binder is 5 to 6 %w/w for solid dosage forms. The outcomes of the study suggested that isolated polysaccharides is promising binding agent as a pharmaceutical excipient in drug delivery...
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Posted by admin on Jul 31, 2017 in |
A sensitive and high throughput high performance liquid chromatography tandem mass spectrometry (HPLC-MS/MS) method has been developed for estimation of methotrexate in human plasma. The sample extraction of methotrexate and methotrexate-d3 (Internal standard) was done from 200 µL of human plasma using solid phase extraction procedure. The chromatographic retention was done on Thermo Butyl Hypersil, 50 x 4.6 mm, 5µm column under isocratic conditions using acetonitrile, 10 mM ammonium formate and 0.5% Formic aid (70:15:15, v/v) as the mobile phase. The mass transition for methotrexate (m/z 455.3 → 308.2), and IS (m/z 458.3 → 311.2) were monitored in the positive ionization mode. The method was validated over a wide-ranging concentration of 0.500-1000 ng/mL. The extraction recovery for the analyte and internal standard was 85.6 %. Stability of methotrexate in plasma was examined under different storage conditions like bench top, wet extract, freeze-thaw and long-term stability. The validated method was used to support a bioequivalence study of 15 mg methotrexate tablet in 16 adult patients. The method reproducibility was proved...
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