Posted by admin on Jul 31, 2017 in |
Cyclic adenosine monophosphate (cAMP) dependent protein kinase plays a major role in cell signaling pathway. Over expression of extracellular cAMP dependent protein kinase catalytic subunit caused tumorgenesis in prostate. This protein may provide a base for the design of clinically applicable therapeutic strategies. In order to develop potential inhibitor of cAMP dependent protein kinase catalytic subunit, a high throughput virtual screening of zinc natural compounds database was conducted. Furthermore, QikProp, ADMET predictor and MM-GBSA was performed for ADME (Absorption, Distribution, Metabolism and Elimination), toxicity and binding energy prediction for ligands, respectively. Finally, molecular dynamics simulation was performed to get potential inhibitor. The crystal structure of cAMP dependent protein kinase was used for current study. The entire library of natural compounds was screened using HTVS for the primary level of screening. The binding of the compounds was studied using standard precision followed by Extra Precision algorithm of Glide Docking. On the basis of docking scores top ten hit compounds were selected for further analysis. The Binding affinity was further calculated...
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Posted by admin on Jul 31, 2017 in |
Prunus cornuta (family Rosaceae) commonly known as ‘Himalayan Bird Cherry’, is a deciduous tree growing to 15 m (49ft 3in) at elevations of 2100-3500 meters. It is hardy to zone (Uttarakhand) 5. It is in flower in May, and the seeds ripen from July to October. The flowers are hermaphrodite and are pollinated by insects and birds. It can grow in semi-shade (light woodland) or no shade. It prefers moist soil. Plant extract (sample named PC-1) of Prunus cornuta bark was prepared in methanol and the prepared extract was subjected to Phytochemical screening which shows the presence of steroids, terpenoids, alkaloids, tannin, saponin, carbohydrate, protein etc. The methanolic extract ( sample named PC-1) of powdered bark of Prunus cornuta were examined for their antimicrobial activity using agar well diffusion method against different pathogenic bacterial stains like Staphylococcus aureus, Pseudomonas aeruginosa, Aspergillus niger and Candida albicans. Thus minimum inhibitory concentration(MIC) of resulting extract were also checked. The sensitivity of the pathogens was also checked with standard antibiotics erythromycin, Fluconazole, and...
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Posted by admin on Jul 31, 2017 in |
Members of the tribe Anthemideae (Asteraceae) attract considerable medicinal attention worldwide and are used for the treatment of many infectious and degenerative diseases in humans due to their biologically active compounds. The aim of this study was to evaluate the antioxidant property of total phenolic compounds (TPC) and flavonoids (TF) in the capitula of 41 representatives and endemic taxa belonging to seven genera (Achillea, Anthemis, Artemisia, Glebionis, Matricaria, Tanacetum and Tripleurospermum) native to Turkey. The results of the experiments showed that the phenolics of the taxa were significantly correlated and associated with their antioxidant capacity values. Our findings will be useful to researchers and others who are interested in the antioxidant potentials of the herbs as dietary supplements. This is the first report to describe the phenolic compound and antioxidant capacity values of capitula only in selected taxa of...
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Posted by admin on Jul 31, 2017 in |
A simple, precise and accurate RP-HPLC method was developed for the determination of Plerixafor in bulk and pharmaceutical dosage forms. The estimation was carried out on Xterra RP 18 (4.6 x 250mm, 5µm) column using a mixture of Methanol: Water (50:50% v/v) as the mobile phase at a flow rate of 0.8ml/min, the detection was carried out at 215nm. The method was validated for linearity, accuracy, precision, specificity, limit of detection and limit of quantification and robustness as per ICH norms. The retention time of the Plerixafor was 5.481 min. The method produce linear responses in the concentration range of 10-50mg/ml with correlation coefficient (r2) of 0.999. The proposed method is useful for the estimation of plerixafor in its pure and injection dosage...
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Posted by admin on Jul 31, 2017 in |
Transition metal doped zinc oxide particles were synthesized in aqueous phase adopting a low cost, easy and environmental friendly route. The precursors used in the preparation of Co doped ZnO (Zn0.95Co0.05O) and ZnO (Zn0.9oCo0.1O) by wet process are zinc acetate dehydrates cobalt chloride, sodium hydroxide, di-ethylene glycol and double distilled water. The doped ZnO particles were characterized by using scanning electron microscope (SEM), energy dispersive X-ray (EDX), Fourier transforms infrared spectroscopy (FTIR). The study constitutes the basis for developing versatile applications of transition metal doped ZnO...
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