Posted by admin on Mar 31, 2024 in |
Cancer is the uncontrolled growth of abnormal cells in the body. It is the second leading cause of death globally, accounting for an estimated 9.6 million deaths in 2018. Topoisomerases are important cellular targets especially in the treatment of human cancers. They are of two types mainly topoisomerase II alpha and beta. Some of the most powerful anticancer drugs used clinically such as etoposide, teniposide, doxorubicin, daunorubicin, mitoxanrone, Amascarine etc act by causing DNA disorders. Topoisomerase II alpha is the target of action selected in this present study. Doxorubicin, one of the potent anticancer drugs that can be used to treat many cancers by acting on topoisomerase II alpha. Benzimidazole and pyrazole is an organic compound that is heterocyclic in nature. These are important pharmacophores and privileged structures in medicinal chemistry. It possess pharmacological activities such as antimicrobial, antiviral, anticancer, antiinflammatory, analgesic, antifungal, antitubercular, anti-convulsant, ACE-inhibitory etc. This study evaluates the anticancer activity of benzimidazole and pyrazole hybrid derivatives on 4FM9 using docking and molecular dynamic...
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Posted by admin on Mar 31, 2024 in |
A simple, precise, and rapid ultra-performance liquid chromatography coupled with tandem mass spectrometry (UPLC-MS/MS method for determination of nitisinone in 20 µl human plasma was developed and validated. Nitisinone 6C13 was used as an internal standard (IS). Chromatographic analysis was performed on an Atlantis dC18 column (2.1 x 100 mm, 3 µm) using a mobile phase consisting of methanol and 10 mM ammonium-acetate (90:10, v;v) that was delivered at a flow rate of 0.25 ml/min. The eluents were monitored using electrospray ionization in the positive ion mode set at transition set of mass-to-charge (m/z): 330 → 217.92 and 336 → 217.91 for nitisinone and nitisinone-13C6 (internal standard, IS), respectively. The retention time of nitisinone and IS both were about 0.88 minutes, respectively. Relationship between nitisinone concentration and peak area ratio of nitisinone to the IS was linear (R2 ≥ 0.9991) in the range of 2–100 µg/ml and the intra- and inter-day coefficient of variations were 1.9% to 4.5% and 3.2% to 6.2%, respectively. Extraction recoveries for nitisinone and the...
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Posted by admin on Mar 31, 2024 in |
Introduction: Distal femoral fractures are among challenging orthopaedic injury constituting 0.4% of all fractures. Despite recent evaluation in operating techniques and surgical implants, debate continues around the choice of implant for various type of distal femur fractures. Method and Material: In prospective randomized study from 2020 to 2022, analysis of 40 patients was done out of which 20 cases were treated in each group with follow-up at 2,6,12,24 weeks and 6 months. Results: Clinical and radiographic evaluation according to KOOS functional score demonstrated that in RL group, 3 case were excellent, 10 cases were good, 07 cases were fair, while in LP group: 01 case was excellent, 07 cases were Good and 11 cases were fair and 01 case was poor. Over 90% of fractures under RN and LP healed within 6 months with average time being 21.15 weeks (range 12 to 28 weeks) in RN and 23.30 weeks (range 12-32 weeks) in LP group. Out of 20 cases of RN group, 2 went for delayed union requiring dynamisation...
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Posted by admin on Feb 29, 2024 in |
Background: Bauhinia purpurea L. is a flowering plant belongs to family Leguminosae, also known as purple orchid plant. All parts of this plant including leaves, stem bark, flower and pods are edible and used for various medicinal purposes. Detailed pharmacognostic profiling of various parts has been performed except plant Pod, thus pod was selected for this study. Objective: The purpose of this work was to enrich the research on B. purpurea L. Pod in respect of pharmacognostical, physicochemical, qualitative identification of secondary metabolites by HPTLC and ethno-botanical profiling. Material and Methods: Entire pod and powder were subject to macro and microscopic study, different physico-chemical parameters including ash, extractive, moisture and pH determined through standard methods with analytical grade chemical and standard equipments. Quantitative estimation of phenolic content was performed by well-known Folin-Ciocalteu method and HPTLC developed through Vision cat software. Result: Fresh pod is green, elongated with sub-orbicular seeds. TS show different parenchymatous arrangement with yellow brown tannin content and Ca-oxalate crystals. Alkaloids, tannin, phenolic and flavonoids were reported...
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Posted by admin on Feb 29, 2024 in |
The quantitative measurement of Axitinib and Avelumab has been created using a simple, quick, precise, sensitive, and reproducible reverse-phasehigh-performance liquid chromatography (RP-HPLC) method. It is more difficult to analyse varying amounts of pharmaceutical active medicinal ingredients in dose forms without any interferences. Therefore, the objective of the current work is to estimate Axitinib and Avelumab simultaneously by adopting an Analytical Quality by Design (AQbD) arotatable central composite-based technique using RP-HPLC-based method development and validation. Axitinib and Avelumab were separated by chromatography using a Hyperclone 5µ BDS C18 130A (150 x 4.6 mm, 5μ) column and a mobile phase made up of Acetonitrile: 0.1% TEA pH-2.5/OPA in a ratio of 45:55 v/v. The flow rate was 1.2 ml/min, and a Photodiode Array Detector operating at room temperature was used to detect absorption at 219 nm. ICH criteria have been used to validate the offered techniques’ linearity, accuracy, precision, and other attributes. The degradation study’s findings showed that the medications deteriorated in high-stress situations. The chemical and pharmaceutical sectors might easily...
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