Posted by admin on Jun 30, 2024 in |
Celiac disease is a multisystem immune based disorder that is triggered by the ingestion of gluten in genetically susceptible individuals. Our study was limited to a survey of the prevalence of Celiac disease in adult in two Syrian governorates (Homs and Tartous), Statistics were collected from hospitals and clinics of gastroenterologists and nutritionists, during the period of 2014-2022. 4000 cases of Celiac disease were recorded in these two Syrian governorates with a population of about 5 million people with ages ranging from 20-33, that is 0.08% in these cases. The presence of HLA class II antigens was observed in all people with Celiac disease as HLA DQ2 was found in 82% of celiac disease patients, and HLA DQ8 was found in the rest. It is very important that follow-up showed that the results of antibody tests (Transglutaminase IgA) in patients adhering to the gluten-free diet decreased from 200 to 100 “during a year of treatment” and to 20 “during two years of treatment” in all patients. The decrease in...
Read More
Posted by admin on May 31, 2024 in |
The study was carried out to isolate and characterize pigment producing bacteria from rhizosphere soil. In our study, isolation and characterization of bacteria that produced pigments were performed using serial dilution, spread plating method followed by gram staining and various other biochemical tests (Oxidase, Catalase and IMViC tests). Our study concluded that bacteria isolated from rhizosphere soil are capable of producing pigment which is flexirubin (light orange in colour) and the bacterium belongs to the genus Chryseobacterium. After extracting the pigment, antimicrobial activity was performed, and it showed that the pigment has antimicrobial activity against both bacteria (E. coli and B. sphaericus) and fungi (Candida albicans). At 100µg/ml concentration, the pigment showed highest antioxidant activity with an absorbance of 0.116AU. The anti-inflammatory activity of the pigment was found to be highest at 1200µg/ml with an absorbance of 0.128AU. It is found that the pigment has the ability to increase SPF property of commercially available sunscreen by 0.713%. Thus different potentials of the pigment can be applicable in various industrial...
Read More
Posted by admin on May 31, 2024 in |
Background: NCp7 is a nucleocapsid protein of HIV-1, a retrovirus causing AIDS in human beings. It is a small protein of 72 amino acids that plays an important role in being a chaperone protein involved in viral development. HIV has shown resistance to drugs engineered to treat its infections. Objectives: In this study, a Structure-Activity Relationship analysis of two distinct derivatives based on point pharmacophore was carried out to obtain an NCp7 inhibitor of better performance. Materials and Methods: A Pharmacophore and 3D-QSAR modeling of Pyridinioalkanoyl thioesters and Benzamide-based thiolcarbamates derivatives was performed. Results: A four-point hypothesis of HHRR pharmacophoric feature was determined. A 3D-QSAR model was built which was validated through regression analysis. Molecular docking often lead compounds showed that the compound ZINC65398698 possesses a higher affinity towards NCp7 protein and binds well whereas other leads possessed only moderate binding affinity. The ADME/T prediction of all the ten lead compounds showed accepted values of biophysical properties. As ZINC65398698 showed higher binding affinity, a molecular dynamics simulation was performed...
Read More
Posted by admin on May 31, 2024 in |
Nano composites of reduced graphene oxide (rGO) and silver nanoparticles (AgNps) were prepared in this work, adopting green reaction conditions. Aloe vera plant extract was used as the stabilizing agent in the AgNps system and graphite precursor has been chosen for rGO preparation adopting mild hydrothermal and sonication methods. AgNps and AgNp–rGO Nano composites are characterized using UV-VIS SPR; powder XRD, HR-TEM and FE-SEM measurements. The catalytic ability of AgNp–rGO nano composites in the as-synthesized form has been confirmed by following the oxidative degradation of the two therapeutically potential drugs such as sodium diclofenac (NaDF) and ranitidine hydrochloride (RHCl) under visible light irradiation in aqueous medium at 25°C. Reaction kinetic parameters and the oxidant peroxo monosulfate (Na2S2O5) effect in presence of AgNp–rGO nano-composites as the catalyst under visible light irradiation exhibited remarkable catalytic behavior in presence of visible light for the degradation of the two drug both in presence and absence of oxidant. However presence of oxidant enhanced the speed of drug degradations with AgNp–rGO nano composites and visible...
Read More
Posted by admin on May 31, 2024 in |
Microbial chitosanase has received greater attention for producing chitooligosaccharides. In this study, chitooligosaccharide hydrolysate was developed using the chitosanase enzyme, and its bioactivity was evaluated. Marine muddy samples proved to be a source of isolating novel chitosan-degrading bacteria when compared to the other samples collected. A chitosan-degrading marine bacterium was isolated, and the characterization of its extracellular enzyme, chitosanase, was studied. The molecular characterization and 16S rDNA sequence evolutionary relatedness of the isolate were carried out. The organism was identified as Brevidomonas diminuta, and its sequence was deposited. The production of chitosanase enzyme was significantly induced by the chitosan substrate, while it reached its peak after 72 hours. pH and temperature have been verified to be ideal for enzyme synthesis at 6.5 and 30 °C, respectively. Utilizing a culture medium containing xylose as the substrate at a level ranging from 1.0 to 1.5% significantly enhanced the enzyme production. The molecular weight of the enzyme, 43 KDa, was ascertained through SDS-PAGE. The pathogen’s growth was suppressed by the chitooligosaccharide hydrolysate...
Read More
