Posted by admin on Sep 30, 2017 in |
An Accurate, sensitive, precise and stability indicating high performance thin layer chromatographic (HPTLC) method has been developed and validated for the estimation of SITA-P. The method was developed using TLC aluminium plates precoated with silica gel 60F254 as the stationary phase using ethyl acetate: methanol: formic acid (8.5:1:0.5 v/v/v) as mobile phase. Densitometric analysis of SITA-P was carried out in the absorbance mode at 265 nm. The retention factor for SITA-P was found to be 0.50 ± 0.04. Linearity was found to be 500 – 2500 ng/band for SITA-P. The method was found to be accurate, precise, and robust according to acceptance criteria. The limit of detection (LOD) and (LOQ) was found to be 124.36 ng/band and 376.87 ng/band for SITA-P respectively. The drug was subjected to stress condition. The method was validated for different parameters as per the International Conference for Harmonization guidelines (ICH). This HPTLC method can be used for the determination for the stability indicating assay methods for bulk drug and its...
Read More
Posted by admin on Sep 30, 2017 in |
The study was to evaluate the hepatoprotective activity of aqueous extract of Pyrus pashia Buch.-Ham. ex D. Don against carbon tetrachloride-induced hepatotoxicity in mice. Hepatotoxicity was induced by CCl4 30% in olive oil (1ml/kg i.p.). Mice were treated with aqueous extract of P. pashia at doses of 250 and 500 mg/kg b.wt. orally for 14 days. There were two group pre-treatment (once daily for 14 days before CCl4 intoxication) and post-treatment (2, 6, 24 and 48 hr after CCl4 intoxication) groups. The observed effects were compared with a known hepatoprotective agent, silymarin. Pre-treatment and post-treatment groups of aqueous extract of P. pashia significantly reduced elevated serum levels of serum transaminases, alkaline phosphatase, and bilirubin and increased the level of total protein as compared to CCl4 treated group. The histopathological study also confirms the hepatoprotection. Preliminary qualitative phytochemical analysis of the plant revealed the presence of carbohydrates, phenolic compounds, tannins, and saponins. The data are expressed as ± S.E.M. one-way analysis of variance (ANOVA) followed by multiple comparisons with the...
Read More
Posted by admin on Sep 30, 2017 in |
Shigellosis becomes a major health problem of developing countries. It is caused by Shigella spp. Shigella dysenteriae causes the most severe; prolonged and fatal Shigellosis by its ability in producing Shigatoxin (Stx). Unfortunately, there has not been any effective vaccine available in worldwide and using antibiotic is no longer effective. Adhesion molecule of bacteria can be used as component of vaccine as its facilitates attachment into host in which the adhesion molecule located in Outer Membrane Protein (OMP). This research aims to confirm the molecular weight (MW) of OMP’s sub unit protein that acts as an adhesion molecule of S.dysenteriae. OMP was isolated using NOG 0.05% and profiled using SDS PAGE. Dominant sub unit protein was purificated using electroelution method. According to Hemagglutinin Assay (HA), sub unit protein with MW 35.47 kDa has the ability to agglutinate mice eritrocyte in the highest titer compared to another sub unit protein. Various concentrations of 35.47 kDa protein (100µg, 50µg, 25µg, 12.5µg dan 6.75µg and 0µg) were used in the assay to...
Read More
Posted by admin on Sep 30, 2017 in |
This investigation was aimed to study the effect of few selected antioxidants, Vitamins and minerals for prevention of gastric erosion in indomethacin induced gastric mucosal damage in rats. Male Wistar rats, weighing between 200 -250 g were divided into 9 groups of 6 animals each (n = 6). The groups were treated respectively as follows Group I normal control and Group II disease control received normal saline, Group III was treated with standard drug Omeprazole, Group IV to IX received test substances for 7 days. Ulceration was induced by a single oral administration of indomethacin (30 mg/kg). Various parameters like, the volume, pH of gastric juice, total acidity, ulcer index, percentage protection, mucin content, pepsin activity and antioxidant enzymes were estimated. Histopathology of stomach epithelium was observed. The ulcer index and total acidity were significantly reduced (p < 0.05). Increase in pH was observed in ulcer induced rats pre-treated with test substances. Mucin content was found to be restored significantly in all rats pre-treated with test substances, and pepsin...
Read More
Posted by admin on Sep 30, 2017 in |
Obesity refers to abnormal or excessive fat accumulation in the human body which results in health risk. The aim and objective of the present study is to perform in-silico docking analysis of the phytoconstituents identified in two medicinal plants namely Cinnamomum verum (cinnamaldehyde, eugenol, cinnamyl acetate, caryophyllene and cinnamic acid) and Coriandrum sativum (nerolidol, geranyl acetate, decanol and linalool) on HMG CoA reductase an eznynme target for antihyperlipidemic activity. The phytoconstituents of the medicinal plants were retrieved from pubchem chemical database. The target for docking study is selected as 3-hydroxy-3-methylglutaryl-coenzyme A (HMG CoA reductase). The 3D protein structure of the enzyme HMG CoA Reductase is obtained from Protein Data Bank [PDB ID: 3CCT]. In-silico docking analysis was performed by using Molegro virtual docker (MVD). The parameter used for docking analysis are MolDock score, Rerank score and hydrogen bond interactions. The docking score and the binding pattern of the phytoconstituents are compared against the standard drugs. The MolDock score of standard drugs atorvastatin, pitavastatin and simvastatin was found to be...
Read More
